上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

非酒精性脂肪肝大鼠腓肠肌脂肪酸氧化能力的同位素标记实验

仓桢，朱超霞，加孜热亚·再依拿提，浦晓琪，李琴，夏芳珍，陆颖理   

  1. 上海交通大学 医学院附属第九人民医院内分泌科， 上海 200011
  • 出版日期:2015-06-28 发布日期:2015-07-30
  • 通讯作者: 陆颖理, 电子信箱: luyingli2008@126.com。
  • 作者简介:仓桢（1989—）， 女， 硕士生; 电子信箱: cangzhenfly@yeah.net。
  • 基金资助:

    国家重点基础研究发展计划项目（“973”计划）（2012CB524906）

Isotope labeling experiment of fatty acid oxidation in gastrocnemius of rats with nonalcoholic fatty liver

CANG Zhen, ZHU Chao-xia, Jiazireya·Zaiyinati,  PU Xiao-qi,  LI Qin,  XIA Fang-zhen, LU Ying-li   

  1. Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011， China
  • Online:2015-06-28 Published:2015-07-30
  • Supported by:

    National Key Basic Research Program of China, “973”Program，2012CB524906

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摘要：

目的 采用同位素标记法研究非酒精性脂肪肝病（NAFLD）大鼠腓肠肌脂肪酸氧化能力的变化。方法 18只SD雄性大鼠随机分为普食组（n=6）及高脂组（n=12），高脂组大鼠经脂肪供能比为42%的饲料喂养12周成功诱导建立NAFLD模型后，再随机分为NAFLD组和小檗碱干预组（给予盐酸小檗碱灌胃干预16周），每组6只。观察各组大鼠体质量及血脂水平的变化。于干预后16周时，取各组大鼠腓肠肌组织放入含9,10-3H-棕榈酸的培养液中培养，经液体闪烁计数仪检测培养液中已生成氚水（3H2O）的比活度，计算脂肪酸氧化率。结果 干预后16周，NAFLD组大鼠的体质量及血脂水平明显高于普食组和小檗碱干预组（P<0.05）；普食组、NAFLD组和小檗碱干预组腓肠肌脂肪酸氧化率分别为（3.96±1.16）%、（2.13±1.00）%和（3.20±1.47）%，统计学分析结果显示：与普食组比较，NAFLD组腓肠肌脂肪酸氧化率显著降低（P<0.05），小檗碱干预组大鼠腓肠肌脂肪酸氧化率高于NAFLD组，低于普食组，但差异均无统计学意义（P>0.05）。结论 骨骼肌如腓肠肌对脂肪酸的氧化利用减少是非酒精脂肪肝大鼠脂代谢紊乱的一个重要原因，也是治疗非酒精性脂肪肝的靶点。

关键词: 非酒精性脂肪肝病, 同位素标记, 小檗碱

Abstract:

Objective To investigate the changes of fatty acid oxidation in gastrocnemius of rats with nonalcoholic fatty liver disease (NAFLD) by the isotope labeling. Methods A total of 18 male SD rats were randomly divided into the normal group (n=6) and high-fat feed group (n=12). The NAFLD model was successfully established by feeding rats of the high-fat feed group with lipid rich food (the ratio of fat energies is 42%) for 12 weeks. Then the high-fat feed group was randomly divided into the NAFLD group (n=6) and berberine intervention group (n=6, intragastric administration of berberine hydrochloride for 16 weeks). The changes of body weight and blood lipid level of each group were observed. After 16 weeks of intervention, rat gastrocnemius tissues were collected and cultured in 1 μCi 9,10-3H-palmitic acid solution. The oxidation rate of fatty acid was calculated according to the specific activity of 3H2O detected by liquid scintillation counter. Results After 16 weeks of intervention, body weights and blood lipid levels of the NAFLD group were significantly higher than those of the normal group and berberine intervention group (P<0.05). The oxidation rates of fatty acid of the normal group, NAFLD group, and berberine intervention group were (3.96±1.16)%, (2.13±1.00)%, and (3.20±1.47)%, respectively. Results of statistical analysis revealed that compared with the normal group, the oxidation rate of fatty acid of gastrocnemius of the NAFLD group decreased significantly (P<0.05). The oxidation rate of fatty acid of gastrocnemius of the berberine intervention group was higher than that of the NAFLD group and lower than that of the normal group, but the differences were not statistically significant (P>0.05). Conclusion The decrease of oxidation and usage of fatty acid in muscle tissues such as gastrocnemius is an important cause of lipid metabolism disorder of rats with nonalcoholic fatty liver disease. It is a target for the treatment of nonalcoholic fatty liver disease.

Key words: nonalcoholic fatty liver disease, isotope labeling, berberine