上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

氟比洛芬酯对坐骨神经慢性缩窄性损伤模型大鼠肌肉中P物质及神经速激肽1受体表达的影响

毛豪丽，刘春芳，徐辉，姜虹，陈志峰   

  1. 上海交通大学 医学院附属第九人民医院麻醉科， 上海 200011
  • 出版日期:2015-07-28 发布日期:2015-08-27
  • 通讯作者: 陈志峰, 电子信箱: dzfc2013@163.com。
  • 作者简介:毛豪丽(1977—), 女， 主治医师， 硕士; 电子信箱: maohaoli@126.com。
  • 基金资助:

    上海交通大学医学院附属第九人民医院院内课题（JY2011A10）

Effects of flurbiprofen axetil on expressions of substance P and neurokinin-1 receptor in muscle of rats with chronic constriction injury of ischiadic nerve

MAO Hao-li, LIU Chun-fang, XU Hui, JIANG Hong, CHEN Zhi-feng   

  1. Department of Anesthesiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2015-07-28 Published:2015-08-27
  • Supported by:

    Project of Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine， JY2011A10

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摘要：

目的  观察氟比洛芬酯对坐骨神经慢性缩窄性损伤（CCI）模型大鼠疼痛阈值及肌肉中P物质（SP）及神经速激肽1受体（NK1-R）表达的影响。方法  24只雄性SD大鼠随机分为4组，每组6只。A组：氟比洛芬酯治疗CCI大鼠（CCI大鼠暴露并结扎右侧坐骨神经，观察记录术前及术后第3、6、9、12、15日大鼠行为学改变并测定其疼痛阈值，在术后疼痛高峰期给予氟比洛芬酯5 mg/kg尾静脉注射）；B组：未经治疗CCI大鼠；C组：氟比洛芬酯治疗假手术大鼠（假手术大鼠仅暴露但不结扎坐骨神经，术后给予氟比洛芬酯5 mg/kg尾静脉注射）；D组：假手术大鼠。采用免疫组织化学法检测大鼠肌肉中SP及NK1-R表达。结果  术后第15日，A、B组大鼠达到疼痛高峰；A组大鼠经氟比洛芬酯治疗后疼痛阈值显著升高（P<0.05）；C组大鼠给予氟比洛芬酯后疼痛阈值亦升高，但差异无统计学意义（P>0.05）;A组SP及NK1-R的表达呈弱阳性，B组表达为强阳性，两组差异有统计学意义（P<0.05）;C、D两组表达均为弱阳性，差异无统计学意义（P>0.05）。结论  氟比洛芬酯对坐骨神经CCI模型大鼠坐骨神经慢性疼痛有明显的镇痛效果，其机制可能与降低肌肉中SP及NK1-R的表达有关。

关键词: 氟比洛芬酯, 坐骨神经, 慢性缩窄性损伤, P物质, 神经速激肽1受体

Abstract:

Objective  To explore the effects of flurbiprofen axetil (FA) on pain threshold and expressions of substance P (SP) and neurokinin-1 receptor (NK1-R) in muscle of rats with chronic constriction injury (CCI) of ischiadic nerve. Methods  Twenty-four male SD rats were randomly divided into 4 groups and each group has 6 rats. For group A, rats with CCI of ischiadic nerve were treated by FA (The right ischiadic nerve of rats was exposed and ligated. Behavior changes of rats were observed and pain threshold was measured before operation and 3, 6, 9, 12, and 15 d after operation. Rats were injected with FA of 5 mg/kg through tail vein when pain threshold reached peak after operation). For group B, rats with CCI of ischiadic nerve were not treated. For group C, sham operation rats were treated by FA (The ischiadic nerve of rats was exposed but was not ligated. Rats were injected with FA of 5 mg/kg through tail vein after operation). Rats of group D were sham operation rats. Expressions of SP and NK1-R in rat muscle were detected by immunohistochemistry. Results  The pain threshold of groups A and B reached peak 15 d after operation. The pain threshold of group A significantly increased after being treated by FA (P<0.05) and the pain threshold of group C also increased after being treated by FA, but the difference was not statistically significant (P>0.05). Expressions of SP and NK1-R of group A were weakly positive and those of group B were strongly positive, the difference between two groups was statistically significant (P<0.05). Expressions of SP and NK1-R of group C and group D were weakly positive and the difference was not statistically significant (P>0.05). Conclusion  The analgesia effect of FA towards chronic pain of ischiadic nerve of rats with CCI of ischiadic nerve is obviously. The mechanism may be relevant to the decrease of expressions of SP and NK1-R in muscle.

Key words: flurbiprofen axetil, ischiadic nerve, chronic constriction injury, substance P, neurokinin-1 receptor