上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

多通道在体记录技术在杏仁核电点燃癫痫小鼠中的应用

李佳佳1，李永华2，龚海庆2，梁培基2，张溥明2，陆钦池1   

  1. 1.上海交通大学 医学院附属仁济医院神经内科， 上海 200127；  2.上海交通大学 生物医学工程学院， 上海 200240
  • 出版日期:2015-08-28 发布日期:2015-09-30
  • 作者简介:李佳佳（1989—）， 女， 硕士生; 电子信箱: jiajialy@sjtu.edu.cn。
  • 基金资助:

    上海市科委重点基础研究项目（13DJ1400303）；上海市自然科学基金（12ZR1413800）；上海交通大学医工交叉研究基金（YG2012ZD08）；上海交通大学医学院附属仁济医院种子基金（RJZZ13-005）

Application of multi-channel in vivo recording techniques to amygdala-kindling epilepsy mice

LI Jia-jia1, LI Yong-hua2, GONG Hai-qing2, LIANG Pei-ji2 , ZHANG Pu-ming2, LU Qin-chi1   

  1. 1.Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China； 2.School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
  • Online:2015-08-28 Published:2015-09-30
  • Supported by:

    Key Basic Research Project of Shanghai Science and Technology Commission (13DJ1400303)；Natural Science Foundation of Shanghai (12ZR1413800)；Shanghai Jiao Tong University Fund for Interdisciplinary Research for Medical Applications (YG2012ZD08)；Seed Fund of Renji Hospital，Shanghai Jiao Tong University School of Medicine (RJZZ13-005)

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摘要：

目的  将多通道在体记录技术与癫痫模型的构建相结合，为研究癫痫网络机制提供一种新的实验方法。方法  采用多通道在体记录技术同时记录杏仁核电点燃小鼠点燃过程中背外侧杏仁核、海马CA1、初级体感皮层和丘脑背内侧核的电活动。在记录电信号的同时，观察小鼠的行为学状态。结果  在1～5级癫痫发作时，4个记录区域均可记录到后放电，且后放电的持续时间随着癫痫发作级别的增大而增加。癫痫发生和发展过程中伴随频域特异性的电信号动态变化。结论  该实验方法有望成为探索癫痫发作和形成过程中网络机制的一种有价值的研究方法。

关键词: 多通道在体记录技术, 颞叶癫痫, 杏仁核电点燃小鼠

Abstract:

Objective  To provide a new experimental method for studying the mechanisms of epileptic network by integrating the multi-channel in vivo recording techniques and the establishment of epilepsy model. Methods  Multi-channel in vivo recording techniques were adopted to simultaneously record neural activities of the basolateral amygdala, cornu ammonis 1 of hippocampus, primary somatosensory cortex, and mediodorsal thalamus of amygdala-kindling mice. Meanwhile, behaviors of mice were also observed and recorded. Results  The afterdischarge was recorded in four recording regions for epilepsy from grade 1 to 5 and the duration of afterdischarge increased with the grade of epilepsy. The incidence and development of epilepsy accompanied dynamic changes of frequency-specific electrical signals. Conclusion  This experimental method may be a valuable method for study the network mechanism of the ictogenesis and epileptogenesis.

Key words: multi-channel in vivo recording techniques, temporal lobe epilepsy, amygdala-kindling mice