上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

七氟醚预处理对肾缺血再灌注损伤中HIF-2α表达的影响

张燕1,2，詹琼慧1,2，陈珏1,2，郑蓓洁1,2，徐欢1,2，何振洲1,2   

  1. 上海交通大学 医学院 1.附属仁济医院南院麻醉科， 上海 201112； 2.附属仁济医院麻醉科， 上海 200127
  • 出版日期:2015-12-28 发布日期:2016-01-21
  • 通讯作者: 何振洲, 电子信箱: sandyhezz@126.com。
  • 作者简介:张燕（1982—）， 女， 住院医师， 学士; 电子信箱: zhangyan19820912@126.com。
  • 基金资助:

    上海市卫生局科研课题（20124059）。

Effects of sevoflurane pretreatment on expression of HIF-2α for renal ischemia reperfusion injury

ZHANG Yan1,2, ZHAN Qiong-hui1,2, CHEN Jue1,2, ZHEN Bei-jie1,2, XU Huan1,2, HE Zhen-zhou1,2   

  1. 1.Department of Anesthesia, Renji Hospital South Campus, Shanghai Jiao Tong University School of Medicine, Shanghai 201112, China； 2.Department of Anesthesia, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Online:2015-12-28 Published:2016-01-21
  • Supported by:

    Scientific Research Project of Shanghai Municipal Health Bureau, 20124059

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摘要：

目的  探讨七氟醚（Sev）预处理减轻肾缺血再灌注损伤的作用机制以及与缺氧诱导因子-2α（HIF-2α）表达的关系。方法  将野生型小鼠和HIF-2α基因敲除（HIF-2α-/-）小鼠分别随机分为假手术组（对照组）、缺血再灌注组（I/R组）和Sev预处理+缺血再灌注组（Sev+I/R组）。术后取血检测血尿素氮（BUN）和血清肌酐（SCr）的水平，取肾组织检测肾组织的病理变化，Western blotting检测肾组织HIF-2α蛋白的表达水平。结果  与对照组相比，I/R组野生型小鼠和HIF-2α-/-小鼠的BUN和SCr水平显著升高（P<0.001）。Sev+I/R组中，野生型小鼠的BUN和SCr水平显著低于HIF-2α-/-小鼠（P<0.001）。所有小鼠的I/R组的病理检查结果显示存在较严重的急性肾损伤病理特征，但Sev+I/R组野生型小鼠呈现较轻微的病理损伤，并且该组的肾HIF-2α表达水平明显高于对照组和I/R组。而Sev+I/R组HIF-2α-/-小鼠的肾病理损伤相对于I/R组并未减轻。结论  Sev预处理可以保护肾缺血再灌注下的肾功能，其机制可能与上调肾组织HIF-2α的表达有关。

关键词: 七氟醚；肾缺血再灌注；缺氧诱导因子-2&alpha

Abstract:

Objective  To investigate the mechanism of alleviating renal ischemia/reperfusion injury by pretreatment with sevoflurane and the correlation with the expression of hypoxia-inducible factor-2α (HIF-2α). Methods  Wild type mice and HIF-2α knockout (HIF-2α-/-) mice were randomly divided into sham operation group (control group), renal ischemia/reperfusion group (I/R group), and sevoflurane pretreatment+renal ischemia/reperfusion group (Sev+I/R group). Levels of blood urea nitrogen (BUN) and serum creatinine (SCr) were detected in blood samples after operation. Renal tissues were harvested to detect the pathological changes and the protein expression of HIF-2α in renal tissues was detected by Western blotting. Results  Compared with the control group, BUN and SCr levels of wild type mice and HIF-2α-/- mice of I/R group were significantly higher (P<0.001). For Sev+I/R group, BUN and SCr levels of wild type mice were significantly lower than those of HIF-2α-/- mice (P<0.001). Results of pathological examination indicated that all mice of I/R group showed pathological characteristics of severe acute renal injury. However, for Sev+I/R group of wild type mice, only mild pathological injury was found and the expression of HIF-2α  was significantly higher than that of control group and I/R group. But for Sev+I/R group of HIF-2α-/- mice, the pathological injury of kidneys was not alleviated compared with that of I/R group. Conclusion  Sevoflurane pretreatment can protect the renal function for renal ischemia/reperfusion injury, which may be relevant to up-regulating the expression of HIF-2α in renal tissues.

Key words: sevoflurane; , renal ischemia/reperfusion injury; , hypoxia-inducible factor-2α