上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

胃肠安及四藤方对人结肠癌细胞株干细胞CD133+的影响

祝利民，沈克平，周浩，潘传芳，姚琼   

  1. 上海中医药大学附属龙华医院肿瘤科， 上海 200032
  • 出版日期:2016-02-28 发布日期:2016-03-29
  • 作者简介:祝利民(1977—)， 女， 副主任医师， 硕士; 电子信箱: zhulimin2000@sina.com。
  • 基金资助:

    国家自然科学基金青年基金项目(81503430)

Effect of Wei Chang An and Si Teng Fang on stem cells CD133+ of human colon cancer strains

ZHU Li-min, SHEN Ke-ping, ZHOU Hao, PAN Chuan-fang, YAO Qiong   

  1. Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Online:2016-02-28 Published:2016-03-29
  • Supported by:

    National Natural Science Foundation of China, 81503430

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摘要：

目的 观察胃肠安和四藤方对结肠癌细胞株HCT-116、SW480、HT-29及其干细胞CD133+的抑制作用。方法 人结肠癌细胞株（HCT-116、SW480、HT-29）随机分为对照组和不同浓度胃肠安（500 mg/L 和1 000 mg/L）、四藤方（100 mg/L 和200 mg/L）干预组，干预24 h后，CCK-8法测定细胞活力，倒置显微镜观察细胞形态；流式细胞仪分析胃肠安和四藤方对HCT-116、SW480、HT-29中CD133+表达的影响。结果 CCK-8法检测结果显示，胃肠安及四藤方干预HCT-116、SW480、HT-29细胞后，细胞活力均下降且呈剂量依赖性；倒置显微镜观察发现：胃肠安及四藤方干预24 h，HCT-116、SW480、HT-29的细胞数量减少，细胞体积缩小，遮光性差，细胞悬浮、脱落而死亡。流式细胞术分析结果显示：对照组HCT-116、SW480、HT-29中CD133+细胞的比例分别为12.52%、10.98%、2.33%；胃肠安方1 000 mg/L干预24 h后，CD133+细胞比例分别下降为7.49%、5.36%、0.11%；四藤方组200 mg/L干预24 h后，CD133+细胞比例分别下降为7.83%、6.45%、0.18%。结论 不同结肠癌细胞株中CD133+细胞比例有差异。胃肠安及四藤方均具有抑制结肠癌细胞株HCT-116、SW480、HT-29增殖的作用；但在同样的干预时间内，较高浓度胃肠安（1 000 mg/L）及四藤方(200 mg/L)才具有抑制3种细胞株干细胞CD133+表达的作用。

关键词: 胃肠安, 四藤方, 结肠癌干细胞, CD133+

Abstract:

Objective To observe the inhibitory effect of Wei Chang An and Si Teng Fang on colon cancer cell strains HCT-116, SW480, and HT-29 and colon cancer stem cells CD133+. Methods Human colon cancer cell strains HCT-116, SW480, and HT-29 were randomly divided into the control group, WCA group (500 mg/L and 1 000 mg/L), and STF group (100 mg/L and 200 mg/L). After 24 h of intervention, cell viability was detected by CCK-8 assay and cell morphology was observed by the inverted microscope. The effects of WCA and STF on the expression of CD133+ in HCT-116, SW480, and HT-29 were analyzed by flow cytometry. Results The results of CCK-8 assay showed that after HCT-116, SW480, and HT-29 cells were intervened by WCA and STF, their viability decreased in a dose-dependent manner. The observation results of the inverted microscope indicated that after 24 h of intervention by WCA and STF, the number of SW480, HT-29, and HCT-116 cells decreased, the cell volume reduced, light shading weakened, and cells suspended, detached, and died. The results of flow cytometry analysis showed that for the control group, proportions of CD133+ cells in cell strains HCT-116, SW480, and HT-29 were 12.52%, 10.98%, and 2.33%, respectively. After 24h of intervention by WCA of 1 000 mg/L, proportions of CD133+ cells dropped to 7.49%, 5.36%, and 0.11%. After 24 h of intervention by STF of 200 mg/L, proportions of CD133+ cells dropped to 7.83% and 6.45%, and 0.18%. Conclusion Proportions of CD133+ cells in different colon cancer cell strains were different. Both WCA and STF can inhibit the proliferation of colon cancer cell strains HCT-116, HT-29, and SW480. But within the same intervention time, only higher concentration of WCA (1 000 mg/L) and STF (200 mg/L) can inhibit the expression of stem cells CD133+ of the three cell strains.

Key words: Wei Chang An, Si Teng Fang, colon cancer stem cells, CD133+