上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

游离脂肪酸对大鼠肺微血管内皮细胞AQP1的表达调节及其作用机制

章怡苇，田鲲，汪燕，张蓉，王爱忠   

  1. 上海交通大学附属第六人民医院麻醉科，上海 200233
  • 出版日期:2016-03-28 发布日期:2017-06-02
  • 通讯作者: 王爱忠, 电子信箱: w19680420@sohu.com。
  • 作者简介:章怡苇（1990—）， 女， 硕士生; 电子信箱: 1030218497@sjtu.edu.cn。
  • 基金资助:

    国家自然科学基金(81272147)

Regulation of aquaporin 1 expression in rat pulmonary microvascular endothelial cells by free fatty acid mixture and action mechanism

ZHANG Yi-wei, TIAN Kun, WANG Yan, ZHANG Rong, WANG Ai-zhong   

  1. Department of Anesthesiology, Shanghai Sixth Peoples Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • Online:2016-03-28 Published:2017-06-02
  • Supported by:

    National Natural Science Foundation of China,81272147

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摘要：

目的 研究混合游离脂肪酸(FFA)对大鼠肺微血管内皮细胞(PMVECs)中水通道蛋白1（AQP1）的表达调节及其作用机制。方法 Western blotting检测FFA处理不同时间后大鼠PMVECs内AQP1蛋白表达变化；Western blotting和real-time PCR检测不同浓度的FFA处理后大鼠PMVECs内AQP1蛋白和mRNA表达变化；用SB203580抑制p38 MAPK通路，Western blotting检测FFA处理对AQP1表达的影响。结果 FFA处理6、12 h后大鼠PMVECs内AQP1蛋白表达量显著高于空白组（P=0.000,P=0.003）。FFA（200 μmol/L）组和FFA（500 μmol/L）组AQP1蛋白表达量显著高于空白对照组(P=0.007,P=0.002)。FFA（100 μmol/L）组、FFA（200 μmol/L）组和FFA（500 μmol/L）组AQP1 mRNA表达量显著高于空白对照组(P=0.000)。SB203580预处理可抑制FFA诱导的大鼠PMVECs 内AQP1蛋白表达量的增加（P=0.016）。结论 FFA可以上调AQP1的表达，对脂肪栓塞中AQP1介导的肺内水转运过程有一定程度的影响，其调控机制可能与p38 MAPK信号通路活化有关。

关键词: 脂肪栓塞, 肺水肿, 游离脂肪酸, 水通道蛋白1, 大鼠肺微血管内皮细胞；p38 MAPK信号通路

Abstract:

Objective To investigate the regulation of aquaporin 1 (AQP1) expression in rat pulmonary microvascular endothelial cells (PMVECs) by free fatty acid (FFA) mixture and action mechanism. Methods Western blotting was used to detect changes in AQP1 protein expression in rat PMVECs after FFA treatment for different periods. Western blotting and real-time PCR were used to detect changes in AQP1 protein and mRNA expressions in rat PMVECs after being treated with FFA at different concentrations. The effect of FFA treatment on AQP1 expression was detected by Western blotting after p38 MAPK pathway had been suppressed by SB203580. Results AQP1 protein expressions in RAT PMVECs after being treated with FFA for 6 and 12 h were significantly higher than those in the blank control group (P=0.000,P=0.003). AQP1 protein expressions in 200 μmol/L and 500μmol/L FFA groups were significantly higher than those in the blank control group (P=0.007, P=0.002). AQP1 mRNA expressions in 100 μmol/L, 200 μmol/L and 500μmol/L FFA groups were significantly higher than those in the blank control group (P=0.000). SB203580 pretreatment could suppress FFA-induced increase in AQP1 protein expression in rat PMVECs (P=0.016). Conclusion FFA can up-regulate AQP1 expression and affect AQP1-mediated intrapulmonary water transport progress in fat embolisms to a certain extent. The regulation mechanism may be associated with activation of p38 MAPK signaling pathway.

Key words: fat embolism syndrome, lung edema, free fatty acid, aquaporin 1, rat pulmonary microvascular endothelial cells；p38 MAPK signaling pathway