上海交通大学学报(医学版)

• 论著(基础研究) • 上一篇    下一篇

黄麦合剂治疗阳虚所致少弱精症的药效学研究

金冠钦1,2,孙黎2,夏玲红2,林厚文2,张健1,沈金芳2   

  1. 上海交通大学 1.医学院病理生理学教研室 教育部细胞分化与凋亡教育部重点实验室, 上海 200025; 2.医学院附属仁济医院药剂科, 上海 200127
  • 出版日期:2016-04-28 发布日期:2016-05-26
  • 通讯作者: 张健, 电子信箱: mdk0xhi4j@hotmail.com。
  • 作者简介:金冠钦(1986—), 男, 药师, 硕士生; 电子信箱: jinguanqin@outlook.com
  • 基金资助:

    上海市科委科技支撑项目(12401900502)

Pharmacodynamic study on the treatment of yang deficiency-caused asthenospermia or oligospermia with Huangmai mixture

JIN Guan-qin1,2, SUN Li2, XIA Ling-hong2, LIN Hou-wen2, ZHANG Jian1, SHEN Jin-fang2   

  1. 1.Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2.Department of Pharmacy, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Online:2016-04-28 Published:2016-05-26
  • Supported by:

    Technology Support Project of Science and Technology Commission of Shanghai Municipality, 12401900502

摘要:

目的 评价黄麦合剂对阳虚动物模型的保护作用及对雄性大鼠、小鼠生殖功能的影响。方法 建立氢化可的松造成的小鼠阳虚模型、雄性大鼠去势造成的肾阳虚模型,观察黄麦合剂给药后的大鼠血清睾酮含量,生殖系统相关器官及腺体包括前列腺、精液囊、提肛肌等脏器系数,和精子活动能力,以及给药后的雄性小鼠使雌性小鼠受孕的比例。结果 对氢化可的松造成的阳虚模型小鼠以及对大鼠去势肾阳虚模型,黄麦合剂各剂量应用后均能减轻阳虚程度,能够显著增加雄性大鼠精子数及精子生成量,不造成雄性大鼠生殖系统相关器官及腺体的增生,对大鼠的睾酮、精子畸形率及精子活力亦有明显改善作用。结论 黄麦合剂能明显改善氢化可的松造成的阳虚模型小鼠症状;对大鼠去势肾阳虚模型,黄麦合剂各剂量应用后均能减轻肾阳虚程度;黄麦合剂具有温和的生精作用,能显著提升雄性小鼠的生殖能力。

关键词: 黄麦合剂, 药效学研究, 少弱精症, 阳虚模型

Abstract:

Objective To assess the effects of Huangmai mixture on the reproductive function of male rodents and protection of the model of yang deficiency. Methods The hydrocortisone-induced mouse model of yang deficiency and emasculation-caused rat model of kidney-yang deficiency were constructed. The rat serum testosterone level, organ coefficients of reproductive organs and glands such as prostate, sperm bladders, and levator ani muscle, sperm motility, and pregnancy rate of female mice were observed after administration of Huangmai mixture. Results For the hydrocortisone-induced mouse model of yang deficiency and emasculation-caused rat model of kidney-yang deficiency, different doses of Huangmai mixture could reduce the level of yang deficiency, significantly increase sperm counts and sperm production without causing the hyperplasia of reproductive organs and glands of male rats, significantly improve the testosterone level and sperm motility, and decrease the sperm abnormality rate of male rats. Conclusion Huangmai mixture can significantly alleviate the symptoms of hydrocortisone-induced mouse model of yang deficiency. For emasculation-caused rat model of kidney-yang deficiency, different doses of Huangmai mixture can reduce the level of kidney-yang deficiency. Huangmai mixture has a mild spermatogenic effect, which can significantly improve the reproductive capacity of male mice.

Key words: Huangmai mixture, pharmacodynamics study, asthenospermia or oligospermia, model of yang deficiency