上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

调节性B细胞在天疱疮患者外周血中的水平及临床意义

许人超1，潘 萌2   

  1. 1. 上海市嘉定区中心医院皮肤科，上海 201800； 2. 上海交通大学 医学院附属瑞金医院皮肤科，上海 200025
  • 出版日期:2016-08-29 发布日期:2016-08-31
  • 通讯作者: 潘 萌，电子信箱：panmeng@medmail.com.cn。
  • 作者简介:许人超（1986—），女，主治医师，硕士；电子信箱：xurenchao0731@126.com。
  • 基金资助:

    国家自然科学基金（81472875）；国家自然青年科学基金（81402598）；上海市卫生局青年科研项目（20134Y204）；上海市教育委员会高峰高原学科建设计划（20152203）

Number of regulatory B cells in peripheral blood in pemphigus patients and its clinical significance

XU Ren-chao1, PAN Meng2   

  1. 1. Department of Dermatology, Jiading Central Hospital, Shanghai 201800, China; 2. Department of Dermatology, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2016-08-29 Published:2016-08-31
  • Supported by:

    National Natural Science Foundation of China, 81472875; Yong Scientists Project of National Natural Science Foundation, 81402598; Project of Shanghai Health Bureau, 20134Y204；Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support，20152203

PDF (PC)

977

摘要：

目的·探讨天疱疮患者外周血调节性B细胞的水平及其在天疱疮发病中的意义。方法·选取天疱疮患者40例，正常对照组20例，抽取外周静脉血，流式细胞术检测外周血中CD19+ B细胞和刺激5 h、48 h后CD19+IL-10+ B细胞水平；酶联免疫吸附法检测血清中抗dsg1和抗dsg3抗体滴度。结果·天疱疮患者外周血CD19+ B细胞比例与正常对照组差异无统计学意义（P>0.05），CD19+ B细胞在急性发作期、慢性活动期及稳定期的天疱疮患者中差异亦无统计学意义（P>0.05）。体外刺激5 h后，天疱疮患者外周血CD19+IL-10+B细胞水平显著高于正常对照组（P=0.008），但在刺激48 h后，2组差异无统计学意义（P>0.05）。20例寻常型天疱疮患者中，急性发作期和慢性活动期患者的抗dsg3抗体滴度均显著高于稳定期患者（P<0.05）；20例落叶型天疱疮患者中，急性发作期和慢性活动期患者的抗dsg1抗体滴度均显著高于稳定期患者（P<0.05）。结论·天疱疮患者血清中致病性自身抗体滴度水平与B细胞数量无关，与疾病活动期有关；天疱疮患者外周血调节性B细胞功能可能存在缺陷。

关键词: 天疱疮, 调节性B细胞, 致病性B细胞, 抗桥粒芯糖蛋白抗体

Abstract:

Objective · To explore the number of regulatory B cells in peripheral blood in patients with pemphigus and its significance in the incidence of pemphigus. Methods · Peripheral venous blood specimens were collected from 40 patients with pemphigus and 20 healthy controls. Flow cytometry was conducted to measure the number of CD19+IL-10+ B cells after stimulation for 5 and 48 h and the number of CD19+ B cells in peripheral blood. The serum anti-dsg1 antibody and anti-dsg3 antibody titers were measured with ELISA. Results · The difference in the proportion of CD19+ B cells in peripheral blood between pemphigus patients and healthy controls was not statistically significant (P>0.05). There was no significant difference in CD19+ B cells in pemphigus patients at acute onset, chronic active and remittent stages (P>0.05). The number of CD19+IL-10+B cells was significantly higher in pemphigus patients than in healthy controls 5 h after in vitro stimulation (P=0.008). However, the difference between two groups was not statistically significant 48 h after stimulation (P<0.05). In 20 PV patients, the anti-dsg3 antibody titers were significantly higher in patients at acute onset and chronic active stages than in patients at remittent stage (P>0.05). In 20 PF patients, the anti-dsg1 antibody titers were significantly higher in patients at acute onset and chronic active stages than patients in remittent stage (P<0.05). Conclusion · The pathogenic antibody titers in pemphigus patients are not associated with the number of B cells but are associated with disease activity. The function of regulatory B cells in peripheral blood in pemphigus patients may have defects.

Key words: pemphigus, regulatory B cell, pathogenic B cell, anti-dsg antibody