上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

体外授精-胚胎移植患者血清和卵泡液中双酚A水平与妊娠结局的关系

刘斌， 姚宁， 高玉平   

  1. 上海交通大学  医学院附属仁济医院生殖医学中心，上海市辅助生殖与优生重点实验室， 上海 200135
  • 出版日期:2016-10-28 发布日期:2016-11-29
  • 通讯作者: 高玉平， 电子信箱： ginagao53@yahoo.com。
  • 作者简介:刘斌（1985—）， 男， 研究实习员， 硕士； 电子信箱： bingo816@163.com。
  • 基金资助:

    上海市科学技术委员会基金（134119a9502）；国家自然科学基金青年科学基金项目（81401260）

Association of serum and follicular fluid bisphenol A levels with pregnancy outcomes in patients undergoing in vitro fertilization-embryo transfer

LIU Bin， YAO Ning， GAO Yu-ping   

  1. Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200135, China
  • Online:2016-10-28 Published:2016-11-29
  • Supported by:

    Foundation of Shanghai Municipal Science and Technology Committee，134119a9502; Young Scientists Project of National Natural Science Foundation of China, 81401260

PDF (PC)

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摘要：

目的·研究双酚A （BPA）在体外授精-胚胎移植（IVF-ET）患者血清及卵泡液中的水平及其与妊娠结局的相关性。方法·收集2014年1月—2015年12月在上海交通大学医学院附属仁济医院行IVF-ET助孕的138例患者取卵日的血清和卵泡液，用酶联免疫吸附试验分别测定血清及卵泡液中BPA水平，分析两者之间的相关性及其与妊娠结局的关系。结果·138例患者中，血清和卵泡液中同时检测出BPA的60例，血清中BPA平均质量浓度为14.8 ng/mL，卵泡液中平均质量浓度为5.2 ng/mL，差异有统计学意义（P=0.000），且两者呈正相关（r=0.51，P=0.000）。在138例患者中，进行临床移植59例，其中妊娠31例，妊娠率52.5%。在获得妊娠的31例患者中检出BPA者7例，未检出者24例；未检出BPA者妊娠率（77.4%）明显高于检出者（22.6%）；妊娠患者卵泡液中BPA质量浓度（4.6 ng/mL）低于未妊娠患者（10.7 ng/mL），差异有统计学意义（P=0.015）；妊娠患者血清中BPA质量浓度（5.9 ng/mL）小于未妊娠患者（16.7 ng/mL），差异有统计学意义（P=0.005）。血清或卵泡液BPA检出者与未检出者在不孕年限方面比较，差异无统计学意义；BPA检出者年龄大于未检出者，但差异无统计学意义。结论·部分IVF-ET患者血清和卵泡液中可检测出BPA，检出率有随年龄增大而增高的趋势；血清中的BPA水平可预测卵泡液中的BPA水平，血液中BPA水平过高可能影响胚胎着床。

关键词: 双酚A；体外授精-胚胎移植；血清；卵泡液, 妊娠率

Abstract:

Objective·To investigate the bisphenol A (BPA) level in serum and follicular fluid from patients undergoing in vitro fertilization-embryo transfer (IVF-ET) and its association with pregnancy outcomes. Methods·Serum and follicular fluid from 138 patients undergoing IVF-ET in Renji Hospital affiliated to the Shanghai Jiao Tong University School of Medicine between January 2014 and December 2015 were collected on ovum harvest day. BPA levels in serum and follicular fluid were measured using enzyme linked immunosorbent assay method. The association between serum and follicular fluid BPA levels, and BPA levels and pregnancy outcomes were analyzed. Results·Of 138 patients, BPA was detected in serum and follicular fluid from 60 patients with average serum and follicular fluid BPA levels of 14.8 ng/ml and 5.2 ng/mL. The difference was statistically significant (P=0.000). BPA levels in serum and follicular fluid were positively correlated (r=0.51, P=0.000). Of 138 patients, 59 underwent clinical transplantation and 31 (52.5%) of them were pregnant. Among these 31 patients, BPA was detected in 7 patients and was not detected in 24 cases. Patients without detection of BPA had a significantly higher pregnancy rate (77.4%) than patients with detection of BPA (22.6%). Pregnant patients had a lower follicular fluid BPA level (4.6 ng/mL) than patients failed to pregnancy (10.7 ng/mL) and the difference was statistically significant (P=0.015). Pregnant patients had a lower serum BPA level (5.9 ng/mL) than patients failed to pregnancy (16.7 ng/mL) and the difference was statistically significant (P=0.005). There was no significant difference in infertility age between patients with and without detection of BPA in serum or follicular fluid. The average age of patients with detection of BPA was older than that of patients without detection of BPA, but the difference was not statistically significant. Conclusion·BPA can be detected in serum and follicular fluid of some patients undergoing IVF-ET. The detection rate increases with the age. The serum BPA level can predict the follicular fluid BPA level. High serum BPA level may affect embryo implantation.

Key words: bisphenol A, in vitro fertilization-embryo transfer, serum, follicular fluid, pregnancy rate