上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (5): 493-.doi: 10.3969/j.issn.1674-8115.2018.05.002

• 论著·基础研究 • 上一篇    下一篇

碗状纳米粒的制备、表征及载药功能的评价

陈中坚，陆琴，方超   

  1. 上海交通大学基础医学院药理学与化学生物学系，上海 200025
  • 出版日期:2018-05-28 发布日期:2018-05-28
  • 通讯作者: 方超，电子信箱：fangchao32@sjtu.edu.cn。
  • 作者简介:陈中坚（1990—），男，硕士生；电子信箱： zhongjianjustin@163.com。
  • 基金资助:
    国家自然科学基金（81773274）

Preparation, characterization and evaluation of drug-loaded function of nanobowls

CHEN Zhong-jian, LU Qin, FANG Chao   

  1. Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China
  • Online:2018-05-28 Published:2018-05-28
  • Supported by:
    National Natural Science Foundation of China, 81773274

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摘要： 目的 ·制备具有碗状形态的纳米颗粒，建立纳米粒表征方法，测试纳米粒载药及释药效率。方法 ·通过乳液聚合法合成聚苯乙烯纳米粒（ polystyrene nanoparticles，PSNPs），利用该纳米粒的溶胀和 3-(三甲氧基甲硅基 )甲基丙烯酸丙酯与正硅酸乙酯选择性交联的特性，合成花生状纳米粒（ peanuts nanoparticles，PNPs），最后利用聚苯乙烯的溶解性制得碗状纳米粒。动态光散射激光粒度仪分析测定各步骤纳米粒的粒径。透射电子显微镜下观察纳米粒形貌。通过振荡培养箱振荡法制备包载有模型药物盐酸阿霉素的碗状纳米粒，并对纳米粒的载药量和释药速率进行测定。结果 ·分别成功合成了 PSNPs、包覆改性后聚苯乙烯纳米粒、 PNPs、二氧化硅改性后花生状纳米粒及碗状纳米粒。最终制备所得碗状纳米粒粒径为（ 126.7±4.9）nm，Zeta表面电势为（ -30.2±1.1）mV；该纳米粒具有显著的碗状结构，载药方法简单，包封率 51.1%，载药量 9.3%，具有预期的缓释效果。结论 ·制备所得的碗状纳米粒可成功装载药物，实现药物缓释并减少药物残留，可应用于药物缓释递送。

关键词: 溶胀反应, 水解缩合反应, 碗状纳米粒, 缓释

Abstract:

Objective · To prepare nanobowls, establish the method for characterizing nanoparticles, and test drug loading efficiency and release efficiency of nanobowls. Methods · The polystyrene nanoparticles (PSNPs) were prepared by the means of the normal emulsion polymerization. The peanuts nanoparticles (PNPs) were synthesized by the swelling process of PSNPs and the selective crosslinking between 3-(trimethoxysilyl) propyl methacrylate and tetraethylorthosilicate. Finally, the polystyrene was dissolved to obtain nanobowls. Dynamic light scattering (DLS) was used to analyze the size of each kind of nanoparticles. Transmission electron microscope (TEM) was used to observe the morphology of nanoparticles. Nanobowls loaded doxorubicin hydrochloride as a model drug though continuous shaking were used to measure drug loading capacity and release efficiency. Results · PSNPs, coated polystyrene nanoparticles (CPSNPs), PNPs, silica peanuts nanoparticles (Si-PNPs) and nanobowls were synthesized successfully. The size of nanobowls was (126.7±4.9) nm and the Zeta potential was (-30.2±1.1) mV. The final nanoparitcles could be used to load drug easily. The drug loading efficiency and loading capacity reached 51.1% and 9.3%, respectively. Moreover, the nanobowls had the expected sustained release effect. Conclusion · The prepared nanobowls are loaded with drugs successfully, which can release drug slowly and almost completely. The nanobowls can be used for drug release delivery.

Key words: swelling reaction, hydrolytic condensation reaction, nanobowl, sustained release

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