现场快速评估法辅助超声引导下甲状腺细针穿刺的研究

上海交通大学学报（医学版）

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现场快速评估法辅助超声引导下甲状腺细针穿刺的研究

贺玉洁¹(), 樊琳蕊², 张翘楚¹, 张至立¹, 丁珺¹, 施超³, 姚勇强¹, 任宏政¹()

^1.上海市浦东新区公利医院病理科，上海 200135
^2.上海市浦东新区公利医院防保科，上海 200135
^3.上海市浦东新区公利医院内分泌科，上海 200135

收稿日期:2023-06-19 接受日期:2023-07-17 出版日期:2023-08-24
通讯作者: 任宏政 E-mail:13636566182@163.com;13683789899@163.com
作者简介:贺玉洁（1972—），女，主管技师，本科；电子信箱: 13636566182@163.com。
基金资助:
上海市浦东新区卫生系统重要薄弱学科建设项目(PWZbr2022-18);2022年浦东新区卫健委重点专科建设项目(PWZzk2022-05)

Study on ultrasound-guided thyroid fine needle puncture assisted by rapid on-side evaluation

HE Yujie¹(), FAN Linrui², ZHANG Qiaochu¹, ZHANG Zhili¹, DING Jun¹, SHI Chao³, YAO Yongqiang¹, REN Hongzheng¹()

^1.Department of Pathology, Gongli Hospital of Shanghai Pudong New Area, Shanghai 200135, China
^2.Department of Preventive Care, Gongli Hospital of Shanghai Pudong New Area, Shanghai 200135, China
^3.Department of Endocrinology, Gongli Hospital of Shanghai Pudong New Area, Shanghai 200135, China

Received:2023-06-19 Accepted:2023-07-17 Online:2023-08-24
Contact: REN Hongzheng E-mail:13636566182@163.com;13683789899@163.com
Supported by:
Shanghai Pudong New Area Health System Key Weak Discipline Construction Project(PWZbr2022-18);Key Specialty Construction Project of Pudong New Area Health Commission in 2022(PWZzk2022-05)

摘要/Abstract

摘要：

目的·探索现场快速评估法（rapid on-side evaluation，ROSE）辅助超声引导下甲状腺细针穿刺（ultrasound-guided fine needle aspiration cytology，US-FNAC）的临床效果。方法·回顾性分析2019年1月—2022年12月于上海市浦东新区公利医院确诊的甲状腺结节患者资料（n=874）。根据细胞学检测方法分为ROSE+液基薄层细胞学检测（thinprep cytologic test，TCT）组（n=469）和现场细胞涂片（cell smear，CS）+TCT组（n=405）。ROSE+TCT组中，ROSE的组织细胞采样采用迪夫快速染色检测，穿刺直至标本满意；CS+TCT组采用苏木精-伊红染色（hematoxylin-eosin staining，H-E染色）+巴氏液基细胞学染色检测。根据Bethesda报告系统（The Bethesda System for Reporting Thyroid Cytopathology，TBSRTC）细胞学分类标准进行细胞学诊断，比较2种方法的样本细胞不满意度和临床效果。结果·ROSE+TCT组与CS+TCT组的标本不满意度分别为2.4%和14.1%，差异具有统计学意义（P=0.000）。ROSE+TCT组的涂片细胞集中、结构清楚、易于观察。将ROSE+TCT组细胞学诊断结果为Ⅲ级及以上的样本制作为细胞蜡块，以提高后续诊断效率。CS+TCT组细胞涂片因细胞数少未能制作为细胞蜡块。ROSE+TCT组的穿刺次数与CS+TCT组的差异有统计学意义（P=0.011）。结论·现场快速评估法辅助超声引导下甲状腺细针穿刺可现场评估标本的有效细胞数量，现场反馈穿刺医师，可满足病理医师实现准确诊断的细胞采集数量要求，减少穿刺次数和治疗时间，为临床医师的诊断和后续检查起到较好的辅助作用。

关键词: 甲状腺结节, 现场快速评估法, 超声引导, 细针穿刺, 细胞量

Abstract:

Objective ·To explore the clinical effect of ultrasound-guided fine needle aspiration cytology assisted by rapid on-side evaluation (ROSE). Methods ·The data of patients with thyroid nodules diagnosed in Gongli Hospital of Shanghai Pudong New Area from January 2019 to December 2022 were retrospectively analyzed (n=874). According to cytological detection methods, they were divided into ROSE+thinprep cytologic test (TCT) group (n=469) and cell smear (CS)+TCT group (n=405). In the ROSE+TCT group, the tissue and cell samples of ROSE were detected by Diff-Quik staining and continue puncturing until the specimen was satisfied. In the CS+TCT group, the tissue and cell samples were detected by hematoxylin-eosin staining (H-E staining) + Pap staining. Cytologic diagnosis was made according to The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) cytologic classification criteria, and the cell dissatisfaction rates and clinical outcomes of the 2 methods were compared. Results ·The dissatisfaction rates of the ROSE+TCT group and CS+TCT group were 2.4% and 14.1%, respectively, with statistical significance (P=0.000). The smear cells of the ROSE+TCT group were concentrated, and the structure was clear and easy to observe. The samples with a cytologic diagnosis of grade Ⅲ and above were prepared as cell wax blocks to improve the efficiency of subsequent diagnosis. The cells of the CS+TCT group could not produce wax blocks due to the small numbers of cells. The puncture times of the ROSE+TCT group were significantly different from that of the CS+TCT group (P=0.011). Conclusion ·The ultrasound-guided thyroid fine needle aspiration assisted by rapid on-site assessment method can assess the effective number of cells in the specimen on the spot, give feedback to the puncturing doctors on the spot, meet the diagnostic accuracy requirements of pathologists by collecting a sufficient number of cells, reduce the number of punctures and treatment time, and play a good auxiliary role in the diagnosis and follow-up examination of clinicians.

Key words: thyroid nodule, rapid on-side evaluation (ROSE), ultrasonic guidance, fine needle aspiration, cell mass

中图分类号:

581.3

贺玉洁, 樊琳蕊, 张翘楚, 张至立, 丁珺, 施超, 姚勇强, 任宏政. 现场快速评估法辅助超声引导下甲状腺细针穿刺的研究[J]. 上海交通大学学报（医学版）.

HE Yujie, FAN Linrui, ZHANG Qiaochu, ZHANG Zhili, DING Jun, SHI Chao, YAO Yongqiang, REN Hongzheng. Study on ultrasound-guided thyroid fine needle puncture assisted by rapid on-side evaluation[J]. Journal of Shanghai Jiao Tong University (Medical Science).

图/表 4

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Item	ROSE+TCT (n=469)	CS+TCT (n=405)	P value
Gender/[n(%)]			0.892
Male	126 (26.9)	113 (27.9)
Female	343 (73.1)	292 (72.1)
Age/year	42.1±4.0	43.2±3.4	0.358
Thyroid puncture site/[n(%)]			0.781
Left thyroid lobe	276 (58.9)	225 (55.6)
Right thyroid lobe	193 (41.1)	180 (44.4)

Item	ROSE+TCT (n=469)	CS+TCT (n=405)	P value
Gender/[n(%)]			0.892
Male	126 (26.9)	113 (27.9)
Female	343 (73.1)	292 (72.1)
Age/year	42.1±4.0	43.2±3.4	0.358
Thyroid puncture site/[n(%)]			0.781
Left thyroid lobe	276 (58.9)	225 (55.6)
Right thyroid lobe	193 (41.1)	180 (44.4)

TBSRTC classification	ROSE+TCT (n=469)	CS+TCT (n=405)
Ⅰ	8 (1.7)	94 (23.2)
Ⅱ	254 (54.2)	199 (49.1)
Ⅲ	129 (27.5)	72 (17.8)
Ⅳ	68 (14.5)	39 (9.6)
Ⅴ	9 (1.9)	1 (0.2)
Ⅵ	1 (0.2)	0 (0.0)

TBSRTC classification	ROSE+TCT (n=469)	CS+TCT (n=405)
Ⅰ	8 (1.7)	94 (23.2)
Ⅱ	254 (54.2)	199 (49.1)
Ⅲ	129 (27.5)	72 (17.8)
Ⅳ	68 (14.5)	39 (9.6)
Ⅴ	9 (1.9)	1 (0.2)
Ⅵ	1 (0.2)	0 (0.0)

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现场快速评估法辅助超声引导下甲状腺细针穿刺的研究

Study on ultrasound-guided thyroid fine needle puncture assisted by rapid on-side evaluation

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