上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (3): 336-.doi: 10.3969/j.issn.1674-8115.2012.03.021

• 论著（临床研究） • 上一篇    下一篇

甲状腺良恶性病变组织中钠碘同向转运蛋白mRNA的表达变化

何蕊娉1, 吴艺捷2   

  1. 1.上海市第七人民医院内分泌科, 上海 200137； 2.上海交通大学附属第一人民医院内分泌科, 上海 200080
  • 出版日期:2012-03-28 发布日期:2012-03-28
  • 作者简介:何蕊娉（1980—）, 女, 主治医师, 硕士；电子信箱: xiaopitou@hotmail.com。

Expression of sodium iodine symporter mRNA in benign or malignant thyroid diseases

HE Rui-ping1, WU Yi-jie2   

  1. 1.Department of Endocrinology, the Seventh People's Hospital, Shanghai 200137, China；2.Department of Endocrinology, the First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China
  • Online:2012-03-28 Published:2012-03-28

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摘要：

目的 探讨良恶性病变甲状腺组织中钠碘同向转运蛋白（NIS）mRNA的表达变化。方法 取甲状腺切除术中的新鲜组织标本，根据病理学检查结果分为结节性甲状腺肿组、甲状腺腺瘤组、甲状腺癌组和瘤周甲状腺组织对照组，采用Real-Time PCR技术测定各组甲状腺组织中NIS mRNA表达。结果 与对照组相比，结节性甲状腺肿组、甲状腺腺瘤组、甲状腺癌组甲状腺组织中NIS mRNA的相对表达量显著减少（P<0.01）。在不同病变组，甲状腺癌组NIS mRNA的相对表达量下降更为明显（P<001）；结节性甲状腺肿组与甲状腺腺瘤组NIS mRNA的相对表达量比较，差异无统计学意义（P>0.05）。结论 甲状腺良恶性病变均存在NIS mRNA表达的下调，且以恶性病变更为明显。提示甲状腺病变时甲状腺“碘泵”功能存在不同程度的损害。

关键词: 钠碘同向转运蛋白, 甲状腺病变, 实时PCR

Abstract:

Objective To investigate the expression of sodium iodine symporter (NIS) in thyroid tissues in benign or malignant thyroid diseases. Methods Fresh thyroid specimens were obtained from thyroid ablation operation, and were divided into nodular goiter group, thyroid adenoma group, thyroid carcinoma group and control group according to pathological findings. The expression of NIS mRNA in thyroid tissues of each group was detected by Real-Time PCR. Results The relative expression of NIS mRNA in nodular goiter group, thyroid adenoma group and thyroid carcinoma group was significantly lower than that in control group (P<0.01). The relative expression of NIS mRNA in thyroid carcinoma group was significantly lower than that in nodular goiter group and thyroid adenoma group (P<0.01). There was no significant difference in the relative expression of NIS mRNA between nodular goiter group and thyroid adenoma group (P>0.05). Conclusion The expression of NIS mRNA decreases both in benign thyroid diseases and in malignant diseases, especially for the latter, which indicates that “iodine pump” defects exist in different thyroid diseases.

Key words: sodium iodine symporter, thyroid disease, Real-Time PCR