目的 探讨蛋白激酶MPK-1对脂质代谢的影响及可能的作用机制。方法 用秀丽线虫（C.elegans）作为模式生物，采用RNA干扰（RNAi）技术定向干扰mpk-1，sbp-1、daf-2基因表达，油红O和苏丹黑染色观察线虫脂质含量的变化，洗脱脂肪进行定量分析确定脂肪含量的增减。采用RNAi技术结合对线虫体内特定位置绿色荧光蛋白表达变化的观察，分析MPK-1影响脂肪累积的可能机制。结果 干扰mpk-1基因表达后，线虫体内脂肪含量明显增加。荧光显微镜观察发现：脂质合成酶fat-7∷gfp线虫干扰mpk-1基因表达后，荧光增强；表达调控fat-7的转录因子DAF-16荧光蛋白的线虫品系中干扰mpk-1基因表达，可促进DAF-16向核内转移。结论 蛋白激酶MPK-1可能通过DAF-16调控FAT-7的活性，抑制脂肪的合成代谢，减少脂质累积。

Objective To investigate the effect of protein kinase MPK-1 on lipid metabolism, and explore the underlying mechanism. Methods C.elegans was served as model organism, and the expression of mpk-1, sbp-1 and daf-2 genes was interfered with RNA interference (RNAi). The fat contents in C.elegans were observed by Oil-red O staining and Sudan black staining, and were quantified after washing off the dye. Then, the mechanism of effect of MPK-1 on fat accumulation was determined by the combination of RNAi knockdown technique and the green fluorescence change in a specified location. Results The fat content significantly increased after interference of mpk-1 gene expression. Fluorescence microscopy revealed that fluorescence enhancement was detected after interference of mpk-1 gene expression in C.elegans with expression of lipogenic enzymes fat-7∷gfp, and intranuclear transference of DAF-16 was promoted after interference of mpk-1 gene expression in C.elegans with expression of DAF-16 fluorescin regulating fat-7. Conclusion Protein kinase MPK-1 may inhibit FAT-7 activity through DAF-16, and reduce the fat accumulation.