目的 以鼠脑胶质瘤细胞C6和鼠脑内皮细胞bEnd.3为模型，研究载多烯紫杉醇(DOC)的转铁蛋白（TF）修饰脂质体（DOC-TF-LP）的体外抗肿瘤作用。方法 采用薄膜分散法制备普通脂质体（LP），后插入法制备TF修饰LP（TF-LP）。荧光定量实验观察C6细胞和bEnd.3细胞对TF-LP和LP的摄取。MTT实验检测不同LP对肿瘤细胞的增殖抑制作用。构建C6细胞肿瘤球模型，观察不同LP对肿瘤球的穿透能力以及对肿瘤球体积的增长抑制能力。结果 脑胶质瘤C6细胞对TF-LP的摄取效率是LP的3.2倍（P<0.05）；脑内皮细胞bEnd.3对TF-LP的摄取效率是LP的2.6倍（P<0.05）。DOC-TF-LP对肿瘤细胞的增殖抑制作用显著大于DOC-LP和DOC游离溶液（P<0.05）。载香豆素-6的TF-LP与肿瘤球共孵育4 h后，TF-LP的荧光强度强于LP。DOC游离溶液、DOC-LP和DOC-TF-LP分别使肿瘤体积减小到原体积的80%、65%和38%。结论 与DOC和DOC-LP相比，DOC-TF-LP能够更加有效地被脑内皮细胞和肿瘤细胞摄取，抑制肿瘤细胞增殖及肿瘤球生长，具有更强的抗肿瘤作用。

Objective To determine the anti-tumor effect of the novel docetaxel (DOC) loaded transferrin modified liposome (DOC-TF-LP) on rat glioma cell line C6. Methods LP

was prepared by film-ultrasonic method. Cellular uptake by brain capillary endothelial cells (bEnd.3) and C6 glioma cells was observed by fluorescence quantitative experiment. The anti-proliferation efficiency of DOC-TF-LP was evaluated by MTT assay. Tumor spheroids were used to evaluate the targeting efficiency and anti-tumor ability of DOC-TF-LP. Results Efficiency of TF-LP cellular uptake by C6 glioma cells was 3.2 times of LP (P<0.05). Efficiency of TF-LP cellular uptake by bEnd.3 was 2.6 times of LP (P<0.05). The anti-proliferative activity of DOC-TF-LP were higher than those of DOC-LP and DOC (P<0.05). Fluorescence intensity of TF-LP was higher than that of LP after incubation with C6 tumor spheroids. Gross tumor volumes decreased to 80%, 65%, and 38% after incubation with DOC, DOC-LP, and DOC-TF-LP, respectively. Conclusion Compared to DOC and DOC-LP, DOC-TF-LP could more persistently inhibit the proliferation of C6 cells and C6 tumor spheroids. Thus, DOC-TF-LP, as a new nanometer drug, has a special application value for the therapy of glioma.