不同培养基对血液透析用水细菌菌落检测比较
网络出版日期: 2014-01-29
基金资助
上海市卫生局基金(2010120)
Comparison of different culture media on bacterial colony count in hemodialysis water
Online published: 2014-01-29
Supported by
Shanghai Municipal Health Bureau Foundation,2010120
目的 比较各种培养基在不同培养温度和时间条件下对血液透析用水的细菌检出率,探索临床上合适的透析用水细菌菌落检测方法。方法 2012年1—12月随机采集上海交通大学医学院附属仁济医院血液净化中心透析用水样本共152份。分别接种在血培养基、胰蛋白酶大豆琼脂(TSA)、Reasoner′2A琼脂(R2A)和胰蛋白胨葡萄糖浸膏琼脂(TGEA)的4种培养基,分别在20 ℃、30 ℃和35 ℃培养48~168 h。培养结束时对每个培养皿进行细菌菌落计数。结果 血培养基组透析用水细菌菌落计数和细菌检出率最低,与TGEA和R2A相比差异具有统计学意义(P<0.05和P<0.01)。R2A在20 ℃环境下培养168 h,细菌检出率较血培养35 ℃培养48 h显著增加(67.1% vs 43.4%, P<0.01)。Bland-Altman分析显示,R2A培养基20 ℃培养168 h与TGEA培养基30 ℃培养120 h两组细菌检出率具有较好的一致性。结论 使用R2A或TGEA培养基较血培养基显著提高透析用水细菌菌落计数和细菌检出率。临床上适合选用R2A培养基20 ℃、168 h或TGEA培养基30 ℃、120 h方法对血液透析用水进行细菌菌落检测。
蔡 宏 , 张伟明 , 杨海慧 , 等 . 不同培养基对血液透析用水细菌菌落检测比较[J]. 上海交通大学学报(医学版), 2014 , 34(1) : 56 . DOI: 10.3969/j.issn.1674-8115.2014.01.012
Objective To compare detection ratio of bacterial colony count in dialysis water with different media and different incubation conditions, and to search for suitable method to detect bacteria from dialysis water. Methods Between January and December of 2012, 152 samples of hemodialysis water from Hemodialysis Center of Renji Hospital were randomly collected. The samples were cultured in duplicate on spread plates with blood agar, tryptic soy agar (TSA), Reasoner′s 2A (R2A), and tryptone glucose extract agar (TGEA), respectively, at 20, 30, and 35 ℃ for 48-168 h. After incubation, the numbers of colonies were quantified. Results In blood agar, the bacterial colony counts of dialysis water were the lowest with significant differences compared to TGEA and R2A culture media (P<0.05, P<0.01). In R2A agar, the detection rate of bacteria at 20 ℃ for 168 h was significantly higher than that in blood agar at 35 ℃ for 48 h (67.1% vs 43.4%, P<0.01). Bland-Altman analysis showed that there was close consistence between the detection rates of bacteria in R2A agar at 20 ℃ for 168 h and in TGEA agar at 30 ℃ for 120 h. Conclusion The method of R2A or TGEA agar culture could improve the detection rate of bacterial colony count in dialysis water in comparing to the method of blood culture. The method of R2A at 20 ℃ for 168 h or TGEA at 30 ℃ for 120 h is the suitable method for bacteria detection in dialysis water.
Key words: bacterial culture; hemodialysis; culture medium; dialysis water
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