目的 探讨正常大鼠肝脏祖细胞的分离和体外培养方法。方法 选择正常Wistar大鼠，以CD90.1为标志物，通过免疫磁珠法分离正常肝脏内的祖细胞。对祖细胞进行体外培养，并诱导分化。结果 CD90.1阳性细胞占肝脏非实质细胞的（0.32±0.03）%，流式细胞仪分析显示CD90.1阳性细胞占分离细胞的（98.26±1.37）%。刚分离的CD90.1阳性细胞中，CK-19表达呈强阳性，HNF-4a表达呈阴性；培养8周后，CK-19表达呈阴性，HNF-4a表达呈阳性。结论 正常大鼠肝脏内存在祖细胞，可将其诱导分化为肝细胞。

Objective To explore the techniques of isolation and in vitro culture of hepatic progenitor cells of normal rat liver. Methods According to the cell surface marker CD90.1, hepatic progenitor cells of normal Wistar rats were isolated by immunomagnetic cell sorting, cultured in vitro, and induced to differentiate. Results The percentage of CD90.1 positive cells in non-parenchymal cells was (0.32±0.03) %. The flow cytometric cell sorting showed that (98.26±1.37) % of isolated cells were CD90.1 positive cells. Fresh isolated CD90.1 positive cells were strong positive for CK-19 and negative for HNF-4a. After 8 weeks of culture, these cells were negative for CK-19 and positive for HNF-4a. Conclusion Hepatic progenitor cells exist in normal rat livers and can be differentiated toward hepatocytes.