网络出版日期: 2014-12-02
基金资助
上海高校青年教师培养资助计划(2012-046)
Expression and biologic effects of microRNA-375 in triple negative breast cancer
Online published: 2014-12-02
Supported by
Cultivation Program for Young Teachers in Shanghai Colleges, 2012-046
目的 探讨微RNA-375 (miRNA-375) 在三阴性乳腺癌中的表达及生物学效应。方法 采用Real-time PCR技术检测62例三阴性乳腺癌患者的癌组织及其癌旁正常组织中miRNA-375的表达,分析miRNA-375表达与临床病理学特征的关系。对乳腺癌细胞株MDA-MB-231和乳腺纤维腺瘤细胞株MCF-10A中miRNA-375的表达进行检测;将miRNA-375前体转染MDA-MB-231,CCK-8法和Transwell实验检测细胞增殖和侵袭力的变化。结果 三阴性乳腺癌组织中miRNA-375表达显著低于癌旁正常组织(P<0.001),miRNA-375的表达与肿瘤的大小和是否存在腋窝淋巴结转移有关(P<0.001)。与MCF-10A比较,MDA-MB-231中miRNA-375的表达显著降低(P=0.021)。miRNA-375前体转染后,MDA-MB-231内miRNA-375表达显著上调,细胞增殖能力和侵袭力减弱。结论 三阴性乳腺癌组织和MDA-MB-231中miRNA-375的表达下调。miRNA-375对三阴性乳腺癌发生和发展具有一定的抑制作用。
杨 晨 , 虞 佩 , 李济宇 , 等 . 微RNA-375在三阴性乳腺癌中的表达及生物学效应[J]. 上海交通大学学报(医学版), 2014 , 34(11) : 1586 . DOI: 11.3969/j.issn.1674-8115.2014.11.005
Objective To investigate the expression and biologic effects of microRNA-375 (miRNA-375) of triple negative breast cancer (TNBC). Methods The expression of miRNA-375 of tumor tissue and adjacent normal tissues of 62 patients with TNBC was detected by the Real-time PCR. The relationship of the expression of miRNA-375 and clinicopathological features was analyzed. The expressions of miRNA-375 in breast cancer cell line MDA-MB-231 and breast fibroadenoma cell line MCF-10A were detected. The miRNA-375 precursor molecule was transfected to MDA-MB-231. The variations of the proliferation and invasion of MDA-MB-231 were detected by CCK-8 kit and Transwell test. Results The expression of miRNA-375 of TNBC tissue was significantly lower than that of adjacent normal tissue (P<0.001) and was relevant to the tumor size and axillary lymph node metastasis (P<0.001). Compared to MCF-10A, the expression of miRNA-375 in MDA-MB-231 significantly decreased (P=0.021). After being transfected by the miRNA-375 precursor, the expression of miRNA-375 in MDA-MB-231 significantly increased and the proliferation and invasion abilities decreased. Conclusion The expression of miRNA-375 in TNBC tissue and MDA-MB-231 is down-regulated. And miRNA-375 can inhibit the carcinogenesis and development of TNBC to a certain extent.
Key words: triple negative breast cancer; microRNA-375; invasiveness
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