目的 建立稳定表达荧光素酶的小鼠骨髓间充质干细胞（MSCs）系。方法 用携带荧光素酶基因的慢病毒GV260感染F3代MSCs系OP9细胞，通过嘌呤霉素筛选建立稳定表达荧光素酶的OP9luc+细胞；检测荧光素酶报告基因的活性，观察其表达效果；将OP9luc+细胞经尾静脉注射至C57BL小鼠体内，观察其成像能力。结果 筛选到稳定表达荧光素酶的小鼠MSCs系OP9luc+；活性测定证明其表达效率高；在动物体内可以清晰成像。结论 构建了稳定高表达荧光素酶基因的小鼠MSCs系OP9luc+。

Objective To establish murine bone marrow mesenchymal stem cell lines that can stably express the luciferase. Methods OP9 cells of MSC lines were infected by lentivirus GV260 containing luciferase gene and screened by puromycin for establishing OP9 luc+ cell lines that could stably express the luciferase. The activity of reporter gene of luciferase was detected and its expression effect was observed. OP9 luc+ cells were injected via the tail vein of C57BL mice and imaging ability was observed. Results OP9 luc+ cells that stably expressed luciferase were screened. The activity detection proved that the expression efficiency of OP9 luc+ cells was high. OP9 luc+ cells could form image clearly in vivo. Conclusion Murine bone marrow mesenchymal stem cell lines with stable and high expression of luciferase are established.