论著(基础研究)

成纤维细胞生长因子21对高糖诱导心肌细胞肥大的保护作用

  • 孟哲颖 ,
  • 王 玉 ,
  • 林艳端 ,
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  • 上海交通大学附属第六人民医院超声医学科 上海超声医学研究所, 上海 200233
孟哲颖(1990—), 女, 硕士生; 电子信箱: doubao1223@sjtu.edu.cn。

网络出版日期: 2015-01-29

基金资助

国家自然科学基金(81270208)

Protective effect of fibroblast growth factor 21 on cardiomyocyte hypertrophy induced by high glucose

  • MENG Zhe-ying ,
  • WANG Yu ,
  • LIN Yan-duan ,
  • et al
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  • Department of Ultrasound in Medicine, the Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai Institute of Ultrasound in Medicine, Shanghai 200233,  China

Online published: 2015-01-29

Supported by

National Natural Science Foundation of China, 81270208

摘要

目的 观察高糖诱导原代心肌细胞时成纤维细胞生长因子21 (FGF21)的表达,初步探讨内源性FGF21对高糖诱导心肌细胞肥大的保护作用。方法 原代培养C57BL/6J乳鼠心肌细胞,观察FGF21在不同糖浓度(5、25、33和45 mmol/L)以及不同作用时间(24和48 h)的表达情况。设计并合成可沉默FGF21基因的小干扰RNA(siRNA),观察心肌细胞的形态学变化,并采用Real-time PCR检测心肌细胞肥大表型ANP、β-MHC mRNA的表达变化。结果 FGF21水平随着高糖浓度升高而升高,同一时间点高糖组(33和45 mmol/L)的FGF21水平高于对照组 (P<0.05);FGF21水平随高糖作用时间延长出现先升高后降低的趋势,相同糖浓度组24 h时的FGF21水平升高,而48 h时的FGF21水平较24 h时降低 (P<0.05);高糖组(33和45 mmol/L)心肌细胞FGF21 mRNA表达高于对照组,随高糖作用时间延长心肌细胞FGF21 mRNA表达水平先升高后降低(P<0.05)。FGF21-siRNA处理后,高糖诱导的心肌细胞肥大更为显著,ANP、β-MHC表达水平升高(P<0.05)。结论 高糖时,内源性FGF21表达水平与糖浓度存在一定的依赖性;FGF21可能参与高糖诱导心肌细胞肥大的过程,起到拮抗心肌细胞肥大的保护性作用。

本文引用格式

孟哲颖 , 王 玉 , 林艳端 , . 成纤维细胞生长因子21对高糖诱导心肌细胞肥大的保护作用[J]. 上海交通大学学报(医学版), 2015 , 35(1) : 23 . DOI: 11.3969/j.issn.1674-8115.2015.01.005

Abstract

Objective To observe the expression of fibroblast growth factor 21 (FGF21) for inducing the primary myocardial cells by high glucose and to explore the protection effect of endogenous FGF21 on the cardiomyocyte hypertrophy induced by high glucose. Methods Cardiomyocytes of neonatal rat C57BL/6J were primarily cultured and expressions of FGF21 were detected for different concentrations of glucose (5, 25, 33, and 45 mmol/L) and different exposure time (24 and 48 h). The small interfering RNA (siRNA) for silencing the FGF21 gene was designed and synthesized. Morphological changes of cardiomyocytes were observed and expressions of hypertrophy phenotype ANP and β-MHC mRNA of cardiomyocytes were detected by the Real-time PCR. Results The FGF21 level increased with the concentration of glucose. The FGF21 level of high glucose groups (33 and 45 mmol/L) was higher than that of the control group at the same time point (P<0.05). With the increase of exposure time, the FGF21 level increased first and then decreased. The FGF21 level at 24 h increased and was higher than that at 48 h (P<0.05). The expression of FGF21 mRNA of high glucose groups (33 and 45 mmol/L) was higher than that of the control group. With the increase of exposure time, the expression of FGF21 mRNA increased first and then decreased (P<0.05). After being treated by FGF21-siRNA, the cardiomyocyte hypertrophy induced by high glucose was more significant and expressions of ANP and β-MHC increased (P<0.05). Conclusion The expression of endogenous FGF21 depends on the concentration of glucose to a certain extent under high glucose condition. FGF21 may involve in the process of cardiomyocyte hypertrophy induced by high glucose and protect cardiomyocytes against the hypertrophy.
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