目的 探讨穿心莲内酯（AD）体外对人急性早幼粒细胞白血病NB4细胞增殖和凋亡的影响。方法 以不同浓度的AD处理NB4细胞48 h，MTT比色法检测细胞增殖；AnnexinV/PI双染法检测细胞早期凋亡率；DNA-PI染色法分析细胞亚二倍体峰及细胞周期；Western blotting测定凋亡相关蛋白的表达。结果 5～25 μmol/L浓度范围的AD对NB4细胞有明显的增殖抑制作用，AD作用于NB4细胞48 h的半数抑制浓度(IC50)为(1.98±0.22) μmol/L。随着AD浓度的升高，细胞早期凋亡率及细胞亚二倍体峰比例和细胞凋亡比例均明显增加（P<0.05），Western blotting检测结果显示凋亡相关蛋白表达亦呈现明显活化现象。结论 AD可有效抑制NB4细胞增殖,诱导细胞凋亡,阻滞细胞周期进程。

Objective To investigate the effects of andrographolide (AD) on the cell proliferation and apoptosis of human acute promyelocytic leukemia-NB4 cells in vitro. Methods NB4 cells were treated with AD of different concentrations for 48 h. The cell proliferation was detected by the MTT assay and the early apoptosis rate was determined by the AnnexinV/PI double staining. The subdiploid peak and cell cycle were analyzed by the DNA-PI staining. The expressions of apoptosis related proteins were detected by the Western blotting. Results AD of 5-25 μmol/L significantly inhibited the proliferation of NB4 cells. The half inhibit concentration (IC50) of AD at 48 h was (1.98±0.22) μmol/L. With the increasing of AD concentration, the early apoptotic rate, subdiploid peak proportion, and apoptosis proportion elevated significantly (P<0.05). The results of Western blotting showed that the expressions of apoptosis related proteins were significantly activated. Conclusion AD can effectively inhibit the proliferation of NB4 cells, induce cell apoptosis, and arrest the cell cycle.