上海交通大学学报（医学版）

上海交通大学学报（医学版） >

2015 , Vol. 35 >Issue 4: 470

DOI: https://doi.org/11.3969/j.issn.1674-8115.2015.04.002

论著（基础研究）

真核细胞翻译起始因子5A与自然流产相关性的初步研究

  • 秦晓黎 ,
  • 刘晓瑞 ,
  • 田福举 ,
  • 等
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  • 上海交通大学 医学院附属国际和平妇幼保健院妇产科， 上海 200030
秦晓黎（1978—）， 女， 主治医师， 博士; 电子信箱: qinxlyx@126.com。

网络出版日期: 2015-04-29

基金资助

国家自然科学基金（31171439）；国家杰出青年科学基金（81125004）

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Preliminary study on correlation between eukaryotic initiation factor 5A and spontaneous abortion

  • QIN Xiao-li ,
  • LIU Xiao-rui ,
  • TIAN Fu-ju ,
  • et al
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  • Department of Gynecology and Obstetrics, International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China

Online published: 2015-04-29

Supported by

National Natural Science Foundation of China， 31171439； National Funds for Distinguished Young Scientists of China， 81125004

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摘要

目的 初步探讨真核细胞翻译起始因子5A （eIF5A）的表达与自然流产的相关性。方法 采用免疫荧光染色、real-time PCR和Western blotting技术检测eIF5A-1和eIF5A-2在自然流产模型小鼠、正常生育力对照组小鼠子宫组织以及在自然流产和人工流产蜕膜组织中的表达。结果 小鼠子宫组织及人蜕膜组织中eIF5A-1和eIF5A-2均有表达，但eIF5A-1表达水平明显高于eIF5A-2；流产组织标本中eIF5A-1、eIF5A-2 mRNA及蛋白水平表达均低于对照组。结论 eIF5A表达下调可能与自然流产的发生和发展有关。

关键词： 真核细胞翻译起始因子5A; 自然流产; 动物模型

本文引用格式

秦晓黎, 刘晓瑞, 田福举, 等. 真核细胞翻译起始因子5A与自然流产相关性的初步研究[J]. 上海交通大学学报（医学版）, 2015, 35(4): 470. DOI: 11.3969/j.issn.1674-8115.2015.04.002

Abstract

Objective To investigate the correlation between eukaryotic initiation factor 5A (eIF5A) and spontaneous abortion. Methods Expressions of eIF5A-1 and eIF5A-2 in mouse uterine tissue of spontaneous abortion model and normal pregnancy controls and in decidua tissue of patients undergoing spontaneous abortion and artificial abortion were detected by immunofluorescence staining, real-time PCR, and Western blotting analysis. Results Both eIF5A-1 and eIF5A-2 were detected in mouse uterine tissue and human decidua tissue, but the expression of eIF5A-1 was significantly higher than that of eIF5A-2. The mRNA and protein expressions of eIF5A-1 and eIF5A-2 of the spontaneous abortion group were lower than those of the control group. Conclusion The down-regulation of eIF5A expression may be relevant to the occurrence and development of spontaneous abortion.

Key words： eukaryotic initiation factor 5A; spontaneous abortion; animal model

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