目的 检测CXCL12、CXCR4和胶质细胞纤丝酸性蛋白（GFAP）在大鼠炎性痛模型背根神经节（DRG）中的表达，并观察抑制CXCR4后大鼠卫星胶质细胞（SGC）的激活和疼痛行为的变化。方法 18只大鼠，随机分为空白对照组、假炎症组和炎症组(n=6)，通过右足底皮下注射完全弗氏佐剂（CFA）建立炎性痛模型，术后3 d测痛阈并取DRG行Western blotting检测CXCL12、CXCR4和GFAP的蛋白表达。另22只大鼠，随机分为假炎症组（n=6）、对照组（CFA+生理盐水，n=8)和实验组（CFA+抑制剂组，n=8)。分别在术前，术后1、2、3 d给药前和给药后2 h测痛阈，第3日取材分别行Western blotting和免疫荧光检测GFAP的表达。结果 炎性痛后大鼠同侧DRG中CXCL12、CXCR4和GFAP表达显著上升（P<0.05）。抑制CXCR4后，大鼠痛阈在2 h内缓解（P<0.05），且GFAP活化明显抑制（P<0.05）。结论 CXCL12、CXCR4和GFAP在炎性痛大鼠DRG中上调，抑制CXCR4能短效缓解疼痛，并能抑制DRG中SGC的激活。

Objective To observe the expressions of CXCL12, CXCR4, and glial fibrillary acidic protein (GFAP) in dorsal root ganglion (DRG) of rat inflammatory pain model,  the activation of satellite glial cell (SGC), and variations of pain behaviors after the inhibition of CXCR4. Methods A total of 18 rats were randomly divided into the blank control group, sham-inflammatory group, and inflammatory group(n=6). The model of inflammatory pain was established by subcutaneous injection of complete Freund’s adjuvant (CFA) at the right thenar. Paw withdraw thresholds (PWTs) were measured on day 3 and the DRGs were removed. The protein expressions of CXCL12, CXCR4, and GFAP were detected by the Western blotting. Another 22 rats were randomly divided into the sham-inflammatory group (n=6), control group (CFA+normal saline, n=8), and treatment group (CFA+inhibitor, n=8). PWTs were measured before treatment, 2 h before and after drug injection, and after being treated for 1, 2, and 3 d. Ipsilateral DRGs were removed on day 3 and the expression of GFAP was detected by the Western blotting and immunofluorescence. Results Inflammatory pain significantly up-regulated expressions of CXCL12, CXCR4, and GFAP in ipsilateral DRGs (P<0.05). PTWs decreased within 2 h (P<0.05) and the expression of GFAP significantly decreased after the inhibition of CXCR4 (P<0.05). Conclusion Inflammatory pain up-regulates the expressions of CXCL12, CXCR4, and GFAP. The inhibition of CXCR4 can relieve the inflammatory pain for a short time and suppress the activation of SGC in DRG.