目的 探讨人牙周膜细胞（hPDLCs）和人骨髓间充质细胞（hBMCs）被包含防御素3 （hBD3）基因的慢病毒转染后，其转染效率和成骨情况。方法 通过助转染剂polybrene将包含目的基因和绿色荧光蛋白（GFP）基因的慢病毒转染入hPDLCs和hBMCs，通过RT-PCR和Western blotting检验是否能正常表达hBD3，通过绿色荧光显微镜计数并计算转染效率。同时使用碱性磷酸酶和茜素红染色检测细胞的成骨潜能。结果 包含有hBD3基因和GFP基因的慢病毒对hBMCs和hPDLCs的转染效率分别达到44.44%和28.98%。仅包含有GFP基因的慢病毒对hBMCs和hPDLCs的转染效率分别达到79.94%和31.71%。结论 hPDLCs和hBMCs对hBD3基因的转染效率相近，可作为促使牙周组织再生的理想的种子细胞；被转染后的细胞仍具有成骨能力。

Objective To investigate the transfection efficiency and bone formation of human periodontal ligament cells (hPDLCs) and human bone marrow mesenchymal cells (hBMCs) after being transfected by the lentivirus containing the gene of human β-defensin 3 (hBD3). Methods The lentivirus containing target gene and green fluorescent protein (GFP) gene was transfected to hPDLCs and hBMCs by the transfection agent polybrene. The expression of hBD3 was detected by the RT-PCR and Western blotting. The green fluorescence microscope was adopted to count cells and the transfection efficiency was calculated. The potential of bone formation was detected by the alkaline phosphatase staining and alizarin red staining. Results The transfection efficiencies of lentivirus containing hBD3 and GFP genes towards hBMCs and hPDLCs were 44.44% and 28.98%, respectively. The transfection efficiencies of lentivirus containing only GFP gene towards hBMCs and hPDLCs were 79.94% and 31.71%. Conclusion The transfection efficiencies of hPDLCs and hBMCs towards hBD3 gene are similar. And hPDLCs and hBMCs are ideal candidates for promoting the regeneration of periodontal tissue and transfected cells are still capable of forming bone.