目的 研究混合游离脂肪酸(FFA)对大鼠肺微血管内皮细胞(PMVECs)中水通道蛋白1（AQP1）的表达调节及其作用机制。方法 Western blotting检测FFA处理不同时间后大鼠PMVECs内AQP1蛋白表达变化；Western blotting和real-time PCR检测不同浓度的FFA处理后大鼠PMVECs内AQP1蛋白和mRNA表达变化；用SB203580抑制p38 MAPK通路，Western blotting检测FFA处理对AQP1表达的影响。结果 FFA处理6、12 h后大鼠PMVECs内AQP1蛋白表达量显著高于空白组（P=0.000,P=0.003）。FFA（200 μmol/L）组和FFA（500 μmol/L）组AQP1蛋白表达量显著高于空白对照组(P=0.007,P=0.002)。FFA（100 μmol/L）组、FFA（200 μmol/L）组和FFA（500 μmol/L）组AQP1 mRNA表达量显著高于空白对照组(P=0.000)。SB203580预处理可抑制FFA诱导的大鼠PMVECs 内AQP1蛋白表达量的增加（P=0.016）。结论 FFA可以上调AQP1的表达，对脂肪栓塞中AQP1介导的肺内水转运过程有一定程度的影响，其调控机制可能与p38 MAPK信号通路活化有关。

Objective To investigate the regulation of aquaporin 1 (AQP1) expression in rat pulmonary microvascular endothelial cells (PMVECs) by free fatty acid (FFA) mixture and action mechanism. Methods Western blotting was used to detect changes in AQP1 protein expression in rat PMVECs after FFA treatment for different periods. Western blotting and real-time PCR were used to detect changes in AQP1 protein and mRNA expressions in rat PMVECs after being treated with FFA at different concentrations. The effect of FFA treatment on AQP1 expression was detected by Western blotting after p38 MAPK pathway had been suppressed by SB203580. Results AQP1 protein expressions in RAT PMVECs after being treated with FFA for 6 and 12 h were significantly higher than those in the blank control group (P=0.000,P=0.003). AQP1 protein expressions in 200 μmol/L and 500μmol/L FFA groups were significantly higher than those in the blank control group (P=0.007, P=0.002). AQP1 mRNA expressions in 100 μmol/L, 200 μmol/L and 500μmol/L FFA groups were significantly higher than those in the blank control group (P=0.000). SB203580 pretreatment could suppress FFA-induced increase in AQP1 protein expression in rat PMVECs (P=0.016). Conclusion FFA can up-regulate AQP1 expression and affect AQP1-mediated intrapulmonary water transport progress in fat embolisms to a certain extent. The regulation mechanism may be associated with activation of p38 MAPK signaling pathway.