目的·用秀丽隐杆线虫（Caenorhabditis elegans）作为模式生物，观察pro-1基因对寿命的影响，并初步探究其影响寿命的机制。方法·在线虫上以RNAi技术定向干扰pro-1基因的表达，随后对pro-1沉默表达后的线虫进行寿命分析，检测其在生长发育、运动、饮食以及生殖能力等方面的变化。进一步用RNAi方法结合线虫体内荧光定位技术以及RT-PCR技术等探究pro-1影响寿命可能的分子机制。结果·与对照组线虫相比，干扰 pro-1表达后，沉默组线虫平均寿命延长了14.58%(P=0.002)；在TJ356线虫中干扰pro-1表达后，DAF-16明显进入细胞核，且DAF-16下游靶基因如old-1、sod-3、hsp-16.1、mtl-1、ctl-2等的mRNA水平被上调。结论·pro-1基因通过促进DAF-16进入细胞核并上调其下游靶基因的mRNA水平，从而延长秀丽隐杆线虫的寿命。

Objective·To observe the effects of pro-1 gene on the lifespan of Caenorhabditis elegans (C. elegans) and preliminary explore the molecular mechanisms. Methods·RNAi technique was used to intervene the expression of pro-1 gene in C. elegans. The lifespan of C. elegans with silent pro-1 was analyzed and changes in growth, development, motion, diet, and reproduction were detected. The molecular mechanisms of pro-1 to affect the lifespan were explored using RNAi technique, fluorescence localization technology, and RT-PCR. Results·The lifespan of silence group C. elegans was significantly increased by 14.58% after the expression of pro-1  intervented as compared with controls (P=0.002). DAF-16 translocated into nucleus in C. elegans  of TJ356 strain after intervening the expression of pro-1 and mRNA expressions of downstream target genes such as old-1, sod-3, hsp-16.1, mtl-1 and ctl-2 were up-regulated. Conclusion·pro-1 prolongs the lifespan of C. elegans via promoting the translocation of DAF-16 into nucleus and up-regulating the mRNA expressions of downstream target genes.