论著(基础研究)

白细胞介素-10调控巨噬细胞以促进眼底新生血管形成

  • 隋爱玲 ,
  • 苏婷 ,
  • 高誉硕 ,
  • 朱艳吉 ,
  • 谢冰
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  • 上海交通大学 医学院附属瑞金医院眼科,上海 200025
隋爱玲(1990—),女,硕士生;电子信箱:ailingsui@163.com。

网络出版日期: 2017-03-30

基金资助

国家自然科学基金(81570853)

IL-10 promotes ocular neovascularization by regulating macrophages

  • SUI Ai-ling ,
  • SU Ting ,
  • GAO Yu-shuo ,
  • ZHU Yan-ji ,
  • XIE Bing
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  • Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Online published: 2017-03-30

Supported by

National Natural Science Foundation of China,81570853

摘要

目的 ·研究白细胞介素-10(IL-10)对眼底新生血管(NV)的影响。方法 ·采用免疫荧光、RT-PCR、Western blotting等方法检测在氧诱导视网膜病变(OIR)小鼠模型及VEGF过表达(rho/VEGF)转基因小鼠模型中IL-10的表达。应用IL-10基因敲除鼠,研究IL-10对视网膜、视网膜下及脉络膜新生血管的影响。结果 ·在OIR及rho/VEGF转基因小鼠模型中,IL-10的免疫荧光染色强度增加,IL-10的mRNA表达量也升高。与正常对照组小鼠相比,IL-10基因敲除鼠(IL-10-/-)由OIR诱导的新生血管和激光诱导脉络膜新生血管(CNV)均显著减少。并且,在IL-10基因敲除鼠中,其低氧诱导因子-1α(HIF-1α)以及血管内皮生长因子(VEGF)
和血管内皮生长因子受体1(VEGFR1)的表达均减少。在IL-10基因敲除的OIR小鼠模型视网膜中,巨噬细胞受调控后数量减少。结论 · IL-10通过刺激HIF-1α及VEGF、VEGFR1的表达而促进眼新生血管的形成。IL-10在视网膜缺氧时通过调控巨噬细胞,促进眼新生血管的形成。

本文引用格式

隋爱玲 , 苏婷 , 高誉硕 , 朱艳吉 , 谢冰 . 白细胞介素-10调控巨噬细胞以促进眼底新生血管形成[J]. 上海交通大学学报(医学版), 2017 , 37(3) : 278 . DOI: 10.3969/j.issn.1674-8115.2017.03.002

Abstract

Objective · To investigate the role of interleukin-10 (IL-10) in regulating ocular neovascularization (NV). Methods · Expression of IL-10 was investigated in mice with oxygen-induced retinopathy (OIR) and transgenic mice with VEGF expression in photoreceptors by immunofluorescence, RT-PCR, and Western blotting. Mice deficient in IL-10 were used to test the effect of IL-10 in retinal, sub-retinal, and choroidal NV. Results · In OIR mice and transgenic mice with VEGF expression in photoreceptors, the staining intensity and mRNA expression of IL-10 were increased. Mice deficient in IL-10 showed a significant reduction in ischemia-induced retinal NV, and choroidal NV at rupture sites in Bruch’s membrane. Mice lacking IL-10 showed reduced levels of hypoxia-inducible factor-1α (HIF-1α) and suppression of ischemia-induced expression of VEGF and VEGF receptor 1. Macrophage was regulated and reduced in ischemic retina of mice with IL-10 deficiency. Conclusion · IL-10 stimulates ocular NV through modulation of HIF-1α and its target genes VEGF and VEGF receptor 1. IL-10 promotes ocular NV via macrophage response to retina ischemia.
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