目的 · 分离并研究裂解产超广谱β- 内酰胺酶（ESBLs）大肠埃希菌的噬菌体，为建立大肠埃希菌噬菌体库及应用噬菌体控 制细菌感染提供研究基础。方法 · 将污水与93 株产ESBLs 的大肠埃希菌共培养，采用双层琼脂平板法分离纯化噬菌体，得到噬菌 体 JDEC001，并进行生物学特性研究及测序和基因组比对分析。结果 · 该噬菌体是一种毒性噬菌体，属于有尾噬菌体目短尾噬菌 体科。在pH 值 5 ～ 11 的范围内及在0 ～ 39 ℃时保持较好的活性。测序结果显示其基因组为双链DNA 分子，全长38 745 bp，GC 含量49.93%，预测有46 个开放阅读框，不编码毒力因子和耐药基因。结论 · 分离纯化得到了一株产ESBLs 大肠埃希菌的噬菌体 JDEC001，在较宽的 pH 和温度范围内稳定性良好。

Objective · To isolate phages which can fight against extended-spectrum β-lactamase (ESBLs)-producing Escherichia coli (E. coli), and provide basic research for establishment of E. coli phage library and treatment of bacterial infection.  Methods · Samples collected from sewage were co-cultured with 93 ESBLs-producing E. coli strains. A phage named JDEC001 was isolated by double agar overlay plaque assay. The biological characteristics, complete genome sequence and comparative genome analyses of JDEC001 were studied respectively.  Results · JDEC001 belongs to the lytic phage as a member of the Caudovirales order, Podoviridae family. It has high activity at pH from 5 to 11 and with temperature from 0 to 39 ℃ . Whole-genome sequencing of JDEC001 demonstrated double-stranded DNA genome of 38 745 bp with GC content of 49.93%, which encoded 46 open reading frames. The comparative genomics also showed that there was no virulent genes or antibiotic resistant genes in its genome.  Conclusion · The phage JDEC001 against ESBLs-producing E. coli was isolated and purified, with good stability in a broad range of pH and temperature.