论著(基础研究)

黄酮类化合物影响大麻素受体过表达细胞中 cAMP 含量的初步研究

  • 李婷 ,
  • 刘坚华
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  • 1. 上海交通大学  基础医学院化学教研室,上海 200025;2. 上海市浦东新区周浦医院药剂科,上海 201318
李婷(1979—),女,主管药师,学士;电子信箱:zhoupuliting@163.com

网络出版日期: 2018-01-10

基金资助

国家自然科学青年基金(81402775);高等学校博士学科点专项科研基金(20130073120113)

Preliminary study of flavonoids affecting cAMP level in cannabinoid receptor overexpressed cells#br#

  • LI Ting ,
  • LIU Jian-hua
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  • 1. Department of Chemistry, Basic Medicine Faculty of Shanghai Jiao Tong University, Shanghai 200025, China; 2. Department of Pharmacy, Shanghai Pudong New District Zhoupu Hospital, Shanghai 201318, China

Online published: 2018-01-10

Supported by

National Natural Science Youth Fund of China, 81402775; Special Research Fund for Doctoral Programs in Colleges and Universities, 20130073120113

摘要

目的 · 初步探讨黄酮类化合物对大麻素受体(CBR)过表达细胞中环磷酸腺苷(cAMP)含量的影响。方法 · 构建重组表达质 粒 GV144-CB1R 和 GV144-CB2R;转染 HEK-293 细胞建立 CBR 过表达细胞模型,研究黄酮类化合物对 CBR 过表达细胞中 cAMP 含 量的影响。结果 · 成功构建 CBR 过表达细胞模型。化合物作用结果显示,所测试的化合物在测试浓度下均可使过表达CBR 的 HEK293 细胞中 cAMP 含量显著降低,且对 CB2R 过表达的细胞有一定选择性。结论 · 该研究所使用的黄酮类化合物都可以在 μmol/L 浓度 下降低 CBR 过表达细胞中 cAMP 的含量。

本文引用格式

李婷 , 刘坚华 . 黄酮类化合物影响大麻素受体过表达细胞中 cAMP 含量的初步研究[J]. 上海交通大学学报(医学版), 2017 , 37(12) : 1623 . DOI: 10.3969/j.issn.1674-8115.2017.12.007

Abstract

Objective · To evaluate the effects of flavonoids on cyclic adenosine monophosphate (cAMP) level in cannabinoid receptor (CBR) overexpressed cells.  Methods · Eukaryotic expression vectors GV144-CB1R and GV144-CB2R were constructed, and transfected into HEK-293 cells to establish CBR overexpressed cell lines. Several flavonoid compounds were studied by measuring the change of cAMP level in CBR-overpressed HEK293 cell lines.  Results · The CBR overexpressed cell lines were constructed successfully. cAMP level in CBR-overexpressed HEK-293 cells decreased after incubation with flavonoid compounds. And these effects were selective for CB2R overexpressed cells.  Conclusion · All flavonoids studied exhibited decreasing effect on cAMP level in CBR-overexpressed cells at μmol/L level.
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