上海交通大学学报（医学版）

上海交通大学学报（医学版） >

2018 , Vol. 38 >Issue 2: 151

DOI: https://doi.org/10.3969/j.issn.1674-8115.2018.02.007

论著（基础研究）

体外肺灌注和传统冷保存法对大鼠供肺质量的影响比较

  • 潘旭峰 1* ,
  • 李健 2* ,
  • 潘雁 3 ,
  • 顾畅 1 ,
  • 郭震 2 ,
  • 杨骏 1
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  • 上海交通大学附属胸科医院 1. 胸外科，2. 体外循环室，3. 药剂科，上海 200030
潘旭峰（1982—），男，主治医师，硕士；电子信箱：panxfshch@163.com。李 健（1991—），男，医师，硕士生；电子信箱：1129598625@qq.com。* 为共同第一作者。

网络出版日期: 2018-03-09

基金资助

 上海市卫生和计划生育委员会青年课题（20144Y0169）

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Comparison of ex vivo lung perfusion with cold storage in rat donor lung preservation

  • PAN Xu-feng1* ,
  • LI Jian2* ,
  • PAN Yan3 ,
  • GU Chang1 ,
  • GUO Zhen2 ,
  • YANG Jun1
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  • 1. Department of Thoracic Surgery, 2. Department of Extracorporeal Circulation, 3. Department of Pharmacy, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China

Online published: 2018-03-09

Supported by

Shanghai Municipal Commission of Health and Family Planning Project for Young Scientists, 20144Y0169

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摘要

目的 · 建立稳定的大鼠体外肺灌注（ex vivo lung perfusion, EVLP）模型，并且与传统的冷保存方法进行保存效果的对比。
方法 · 对 8 只 SD 大鼠进行 EVLP 转流 4 h，对另外 8 只进行传统冷保存 3 h+ EVLP 1h。比较 2 组肺生理指标、湿干比、伊文思蓝染
色浓度的差异。结果 · EVLP 组的肺湿干比值显著低于传统冷保存组（5.08±0.88 vs 6.09±0.48, P=0.012），氧合指数显著高于传统冷
保存组 [（337.9±35.5）mmHg vs（300.5±21.6）mmHg，P=0.023]，伊文思蓝浓度显著低于传统冷保存组 [（36.5±20.3）μg/mL vs
（65.0±29.9）μg/mL，P=0.043]。结论 · EVLP 作为传统冷保存法的一种有效替代方法，能够较好地延缓肺部损伤。

关键词：   体外肺灌注；冷保存；肺移植；大鼠

本文引用格式

潘旭峰 1* , 李健 2* , 潘雁 3 , 顾畅 1 , 郭震 2 , 杨骏 1 . 体外肺灌注和传统冷保存法对大鼠供肺质量的影响比较[J]. 上海交通大学学报（医学版）, 2018 , 38(2) : 151 . DOI: 10.3969/j.issn.1674-8115.2018.02.007

Abstract

Objective · To establish stable rat ex vivo lung perfusion (EVLP) model and compare the effect of preservation between EVLP group and cold
storage group. Methods · Eight SD rats were reconditioned with EVLP for 4 h and 8 SD rats were under 3-h cold storage and 1-h EVLP. The biological
index, wet-to-dry weight ratio and concentration of Evans blue were analyzed between two groups. Results · Wet-to-dry weight ratio of lung tissue in
EVLP group was significantly lower (5.08±0.88 vs 6.09±0.48, P=0.012); Oxygenation index in EVLP group was significantly higher [(337.9±35.5) mmHg
vs (300.5±21.6) mmHg, P=0.023]; Concentration of Evans blue in EVLP group was significantly lower [(36.5±20.3) μg/mL vs (65.0±29.9) μg/mL,
P=0.043]. Conclusion · EVLP as an alternative to traditional cold storage technique can effectively alleviate the lung damage.

Key words： ex vivo lung perfusion; cold storage; lung transplantation; rat

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