上海交通大学学报（医学版）

上海交通大学学报（医学版） >

2021 , Vol. 41 >Issue 2: 147 - 153

DOI: https://doi.org/10.3969/j.issn.1674-8115.2021.02.004

论著·基础研究

脐静脉内皮细胞外泌体对炎症因子刺激下前软骨细胞凋亡的影响

  • 杨润泽 ,
  • 许文宁 ,
  • 郑火亮 ,
  • 蒋盛旦
展开
  • 上海交通大学医学院附属新华医院脊柱中心，上海 200092
杨润泽（1994—），男，硕士生，电子信箱：Harry1994@sjtu.edu.cn；|许文宁（1991—），男，博士生，电子信箱：xuwenningyu@163.com。

收稿日期: 2020-05-28

  网络出版日期: 2021-02-28

基金资助

国家自然科学基金面上项目(81672206);上海市教育委员会高峰高原学科建设计划(20181809)

收起

Effects of exosomes derived from human umbilical vein endothelial cells on apoptosis of pre-chondrogenic cells stimulated by inflammatory factors

  • Run-ze YANG ,
  • Wen-ning XU ,
  • Huo-liang ZHENG ,
  • Sheng-dan JIANG
Expand
  • Department of Clinic of Spine Center, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China

Received date: 2020-05-28

  Online published: 2021-02-28

Supported by

National Natural Science Foundation of China(81672206);Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support(20181809)

Fold

摘要

目的·探讨脐静脉内皮细胞外泌体对炎症刺激下小鼠前软骨细胞ATDC5凋亡的影响。方法·采用试剂盒分离人脐静脉内皮细胞（human umbilical vein endothelial cell，HUVEC）外泌体，采用Western blotting检测外泌体标志蛋白肿瘤易感基因101（tumor susceptibility gene 101，Tsg101）、白细胞分化抗原9（cluster differentiation 9，CD9）、凋亡相关基因2互作蛋白X（apoptosis linked gene-2-interacting protein X，Alix）的表达水平。透射电镜观察外泌体形态，粒度检测鉴定外泌体大小。使用荧光显微镜观察外泌体被小鼠前软骨细胞ATDC5摄取过程和活性氧（reactive oxygen species，ROS）的生成情况。TUNEL染色和流式细胞术检测外泌体对白细胞介素-1β（interleukin-1β，IL-1β）刺激下的ATDC5细胞凋亡的影响。Western blotting检测外泌体对于ATDC5细胞中凋亡相关蛋白B淋巴细胞瘤2（B-cell lymphoma/leukemia-2，Bcl-2）、Bcl-2相关X蛋白（Bcl-2 associated X protein，Bax）、裂解的半胱天冬酶3（cleaved caspase-3，c-caspase-3）和抗氧化应激相关蛋白核因子E2相关因子2（nuclear factor E2 related factor 2，Nrf-2）、Kelch样环氧氯丙烷相关蛋白1（Kelch-like ECH-associated protein 1，Keap-1）、血红素加氧酶1（heme oxygenase 1，HO-1）、还原型烟酰胺腺嘌呤二核苷酸醌氧化还原酶1（NADPH quinone oxidoreductase-like protein 1，NQO-1）表达水平的影响。结果·透射电镜下观察到HUVEC来源的外泌体呈椭圆形，中空，双层膜，并且阳性表达外泌体标志物CD9、Alix、Tsg101。与IL-1β刺激下的ATDC5细胞相比，外泌体促进炎症因子作用下的ATDC5细胞内ROS的生成（P=0.000）和凋亡的发生（P=0.000），Bax、c-caspase-3、Keap-1表达升高，Bcl-2、Nrf-2、HO-1、NQO-1表达降低。结论·HUVEC来源的外泌体可能通过抑制ATDC5细胞抗氧化应激的能力，促进IL-1β刺激下ATDC5细胞凋亡的发生。

关键词： 外泌体; 骨关节炎; 前软骨细胞; 脐静脉内皮细胞; 氧化应激; 凋亡

本文引用格式

杨润泽 , 许文宁 , 郑火亮 , 蒋盛旦 . 脐静脉内皮细胞外泌体对炎症因子刺激下前软骨细胞凋亡的影响[J]. 上海交通大学学报（医学版）, 2021 , 41(2) : 147 -153 . DOI: 10.3969/j.issn.1674-8115.2021.02.004

Abstract

Objective

·To investigate the effect of exosomes derived from umbilical vein endothelial cells (HUVECs) on apoptosis of murine pre-chondrogenic cell line ATDC5 cells under inflammatory stimulation.

Methods

·The exosomes derived from HUVECs were isolated by using an exosome isolation kit. Western blotting was used to detect the exosome marker proteins, including tumor susceptibility gene 101 (Tsg101), cluster differentiation 9 (CD9) and apoptosis linked gene-2-interacting protein X (Alix). The morphology of exosomes was observed by transmission electron microscope, and the size of exosomes was identified by particle size detection. Fluorescence microscope was used to observe the ATDC5 cell uptake of exosomes and the production of reactive oxygen species (ROS). TUNEL staining and flow cytometry were used to examine the effect of exosomes on ATDC5 cell apoptosis stimulated by interleukin-1β (IL-1β). Western blotting was used to detect the effect of exosomes on the expression levels of ATDC5 apoptosis-related proteins such as B-cell lymphoma/leukemia 2 (Bcl-2), Bcl-2 associated X protein (Bax), cleaved caspase-3 (c-caspase-3) and anti-oxidative stress-related proteins such as nuclear factor E2 related factor 2 (Nrf-2), Kelch-like ECH-associated protein 1 (Keap-1), heme oxygenase 1 (HO-1) and NADPH quinone oxidoreductase-like protein 1 (NQO-1) under IL-1β stimulation.

Results

·Under the transmission electron microscope, the HUVEC-derived exosomes were oval, hollow, double-layered, and positively expressed exosome markers CD9, Alix and Tsg101. Compared with the ATDC5 cells stimulated by IL-1β, ATDC5 cells stimulated by IL-1β incubated with exosomes had higher level of ROS (P=0.000) and higher apoptosis rate (P=0.000). The expression of Bax, c-caspase-3 and Keap-1 increased, and the expression of Bcl-2, Nrf-2, HO-1 and NQO-1 decreased in ATDC5 cells exposed to IL-1β and exosomes compared to ATDC5 cells only exposed to IL-1β.

Conclusion

·HUVEC-derived exosomes may promote ATDC5 cells apoptosis under the stimulation of IL-1β by inhibiting the ability of ATDC5 cell to resist oxidative stress.

Key words： exosome; osteoarthritis (OA); pre-chondrogenic cell; human umbilical vein endothelial cell (HUVEC); oxidative stress; apoptosis

参考文献

1 Xia BJ, Chen D, Zhang JS, et al. Osteoarthritis pathogenesis: a review of molecular mechanisms[J]. Calcif Tissue Int, 2014, 95(6): 495-505.
2 Felson DT. Osteoarthritis: new insights. part 1: the disease and its risk factors[J]. Ann Intern Med, 2000, 133(8): 635.
3 Pereira D, Ramos E, Branco J. Osteoarthritis[J]. Acta Med Port, 2014, 28(1): 99.
4 Charlier E, Deroyer C, Ciregia F, et al. Chondrocyte dedifferentiation and osteoarthritis (OA)[J]. Biochem Pharmacol, 2019, 165: 49-65.
5 Pap T, Korb-Pap A. Cartilage damage in osteoarthritis and rheumatoid arthritis: two unequal siblings[J]. Nat Rev Rheumatol, 2015, 11(10): 606-615.
6 Theocharis AD, Manou D, Karamanos NK. The extracellular matrix as a multitasking player in disease[J]. FEBS J, 2019, 286(15): 2830-2869.
7 Liu HC, Liu YL, Chen B. Antagonism of GPR4 with NE 52-QQ57 and the suppression of AGE-induced degradation of type Ⅱ collagen in human chondrocytes[J]. Chem Res Toxicol, 2020, 33(7): 1915-1921.
8 Simpson RJ, Lim JW, Moritz RL, et al. Exosomes: proteomic insights and diagnostic potential[J]. Expert Rev Proteom, 2009, 6(3): 267-283.
9 Tran TH, Mattheolabakis G, Aldawsari H, et al. Exosomes as nanocarriers for immunotherapy of cancer and inflammatory diseases[J]. Clin Immunol, 2015, 160(1): 46-58.
10 Barile L, Vassalli G. Exosomes: therapy delivery tools and biomarkers of diseases[J]. Pharmacol Ther, 2017, 174: 63-78.
11 Song H, Li X, Zhao Z, et al. Reversal of osteoporotic activity by endothelial cell-secreted bone targeting and biocompatible exosomes[J]. Nano Lett, 2019, 19(5): 3040-3048.
12 Sáez T, de Vos P, Kuipers J, et al. Exosomes derived from monocytes and from endothelial cells mediate monocyte and endothelial cell activation under high d-glucose conditions[J]. Immunobiology, 2019, 224(2): 325-333.
13 Davies LC, Heldring N, Kadri N, et al. Mesenchymal stromal cell secretion of programmed death-1 ligands regulates T cell mediated immunosuppression[J]. Stem Cells, 2017, 35(3): 766-776.
14 Jun Z, Xinmeng J, Yue L, et al. Jumonji domain containing-3 (JMJD3) inhibition attenuates IL-1β-induced chondrocytes damage in vitro and protects osteoarthritis cartilage in vivo[J]. Inflamm Res, 2020, 69(7): 657-666.
15 Théry C, Ostrowski M, Segura E. Membrane vesicles as conveyors of immune responses[J]. Nat Rev Immunol, 2009, 9(8): 581-593.
16 Ying C, Wang R, Wang Z, et al. BMSC-exosomes carry mutant HIF-1α for improving angiogenesis and osteogenesis in critical-sized calvarial defects[J]. Front Bioeng Biotechnol, 2020, 8: 565561.
17 Liang RM, Zhao JM, Li B, et al. Implantable and degradable antioxidant poly(ε-caprolactone)-lignin nanofiber membrane for effective osteoarthritis treatment[J]. Biomaterials, 2020, 230: 119601.
18 Kong N, Ji XY, Wang JQ, et al. ROS-mediated selective killing effect of black phosphorus: mechanistic understanding and its guidance for safe biomedical applications[J]. Nano Lett, 2020, 20(5): 3943-3955.
19 Osama A, Zhang J, Yao J, et al. Nrf2: a dark horse in Alzheimer's disease treatment[J]. Ageing Res Rev, 2020, 64: 101206.
20 Bollong MJ, Lee G, Coukos JS, et al. A metabolite-derived protein modification integrates glycolysis with KEAP1-NRF2 signalling[J]. Nature, 2018, 562(7728): 600-604.
21 Hou RL, Liu X, Yan JJ, et al. Characterization of natural melanin from Auricularia auricula and its hepatoprotective effect on acute alcohol liver injury in mice[J]. Food Funct, 2019, 10(2): 1017-1027.
22 Wang P, Gao YM, Sun X, et al. Hepatoprotective effect of 2'-O-galloylhyperin against oxidative stress-induced liver damage through induction of Nrf2/ARE-mediated antioxidant pathway[J]. Food Chem Toxicol, 2017, 102: 129-142.
Options
文章导航

/

〈 〉