上海交通大学学报（医学版）

上海交通大学学报（医学版） >

2021 , Vol. 41 >Issue 7: 915 - 919

DOI: https://doi.org/10.3969/j.issn.1674-8115.2021.07.011

论著 · 临床研究

CTRP2与冠状动脉阻塞患者侧支循环形成的临床关联及相关作用

  • 汤静 ,
  • 于晨溪 ,
  • 陈中丽 ,
  • 章安迪
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  • 上海交通大学医学院附属瑞金医院心内科，上海 200023
汤静（1973—），女，硕士生；电子信箱：tj20536@rjh.com.cn。

网络出版日期: 2021-08-03

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Relationship between CTRP2 and coronary collateral circulation

  • Jing TANG ,
  • Chen-xi YU ,
  • Zhong-li CHEN ,
  • An-di ZHANG
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  • Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200023, China

Online published: 2021-08-03

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摘要

目的·探讨C1q肿瘤坏死因子相关蛋白2（C1q/tumor necrosis factor-related protein-2，CTRP2）与冠状动脉阻塞患者侧支循环形成的关联及其作用。方法·选取2019年9月—12月在上海交通大学医学院附属瑞金医院心内科住院并进行冠状动脉造影确诊为冠状动脉阻塞的患者177人，根据Rentrop分级标准，以患者冠状动脉侧支循环情况分级为侧支循环形成不良组和侧支循环形成良好组，采用酶联免疫吸附测定（enzyme linked immunosorbent assay，ELISA）检测患者血浆中脂肪因子补体CTRP2表达水平。构建下肢缺血动物模型，分为对照组和CTRP2过表达组，通过激光多普勒仪测定血供恢复情况，计算血供恢复比率。使用重组CTRP2蛋白以50、100及200 pg/mL的剂量刺激血管内皮细胞，于Matrix Gel上检测血管环形成能力，并通过Transwell检测内皮细胞迁移能力。结果·临床研究发现冠状动脉阻塞患者中侧支循环形成良好患者相较于侧支循环形成不良患者血浆CTRP2水平升高（P=0.009），CTRP2对侧支循环形成不良的风险比OR=0.889，95% CI 0.844~0.936，P=0.000。动物模型研究发现CTRP2可促进小鼠下肢缺血模型血供恢复（P=0.032，P=0.009）。细胞实验发现CTRP2增强内皮细胞成血管和迁移能力，且与其浓度呈正相关（均P<0.05）。结论·冠状动脉阻塞患者中侧支循环形成良好患者血浆CTRP2表达水平升高，CTRP2可促进动物体内侧支循环的建立及体外内皮细胞成血管和迁移。

关键词： CTRP2; 冠状动脉阻塞; 侧支循环; 血管新生

本文引用格式

汤静 , 于晨溪 , 陈中丽 , 章安迪 . CTRP2与冠状动脉阻塞患者侧支循环形成的临床关联及相关作用[J]. 上海交通大学学报（医学版）, 2021 , 41(7) : 915 -919 . DOI: 10.3969/j.issn.1674-8115.2021.07.011

Abstract

Objective

·To explore the relationship between C1q/tumor necrosis factor-related protein-2 (CTRP2) and collateral circulation in patients with coronary artery occlusion.

Methods

·Firstly, 177 patients who were hospitalized in the Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine from September 2019 to December 2019 and were diagnosed with coronary artery obstruction by coronary angiography were selected. According to the Rentrop method, the patients were classified into two groups: poor collateral circulation formation group and good collateral circulation formation group. ELISA was used to detect the level of adipokine complement CTRP2 in patients′ plasma. The animal model of lower limb ischemia was established and divided into contrast group and CTRP2 overexpression group. The recovery of blood supply was measured by laser Doppler instrument and the recovery ratio of blood supply was calculated. Finally, the recombinant CTRP2 protein was used to stimulate vascular endothelial cells at the doses of 50, 100 and 200 pg/mL, and the percentage was detected on Matrix Gel. The migration ability of endothelial cells was detected by Trans-well.

Results

·Clinical study found that the plasma CTRP2 level in patients having coronary artery occlusion with good collateral formation was higher than that in patients with poor collateral circulation (P=0.009), and the risk ratio of CTRP2 to poor collateral circulation was OR=0.889, 95%CI 0.844?0.936, P=0.000. The animal model study found that CTRP2 can promote the recovery of blood supply in mice with lower limb ischemia (P=0.032, P=0.009). Cell experiments showed that CTRP2 enhanced the angiogenesis and migration ability of endothelial cells, and it was positively correlated with its concentration (all P<0.05).

Conclusion

·The level of plasma CTRP2 in patients having coronary artery occlusion with good collateral circulation is increased. CTRP2 can promote the establishment of collateral circulation in vivo and the angiogenesis and migration of endothelial cells in vitro.

Key words： C1q/tumor necrosis factor-related protein-2 (CTRP2); coronary occlusion; collateral circulation; angiogenesis

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