上海交通大学学报（医学版）

上海交通大学学报（医学版） >

2021 , Vol. 41 >Issue 8: 1099 - 1102

DOI: https://doi.org/10.3969/j.issn.1674-8115.2021.08.017

综述

釉基质蛋白衍生物对牙周组织再生相关细胞的生物学作用及其成血管作用的研究进展

  • 庄齐翔 ,
  • 董家辰 ,
  • 束蓉
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  • 上海交通大学医学院附属第九人民医院牙周病科，上海交通大学口腔医学院，国家口腔医学中心，国家口腔疾病临床医学研究中心，上海市口腔医学重点实验室，上海 200011
庄齐翔（1995—），男，中国台湾，硕士生；电子信箱：bensonchuang1995@163.com。
束蓉，电子信箱：shurong1977@163.com。

网络出版日期: 2021-08-13

基金资助

国家自然科学基金(81570977);上海市科学技术委员会资助项目(18ZR1422400)

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Biological and angiogenic effects of enamel matrix derivative on periodontal regeneration-related cells

  • Chi-hsiang CHUANG ,
  • Jia-chen DONG ,
  • Rong SHU
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  • Department of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
SHU Rong, E-mail: shurong1977@163.com.

Online published: 2021-08-13

Supported by

National Natural Science Foundation of China(81570977);Project of Science and Technology Commission of Shanghai Municipality(18ZR1422400)

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摘要

釉基质蛋白经研究证实能促进牙周组织再生，随后其被开发成釉基质蛋白衍生物（enamel matrix derivative，EMD）。目前在临床上，EMD已应用20余年，多项临床研究表示其亦具有较突出的牙周组织再生功能。血管生成是组织再生的关键步骤，即新生血管可将多种细胞因子运送到组织缺损区，以促进组织再生。同时，另有文献指出，EMD有成血管作用。该文就EMD对牙周组织再生相关细胞的作用及其成血管作用进行综述。

关键词： 釉基质蛋白衍生物; 血管生成; 血管内皮细胞; 牙周组织再生

本文引用格式

庄齐翔 , 董家辰 , 束蓉 . 釉基质蛋白衍生物对牙周组织再生相关细胞的生物学作用及其成血管作用的研究进展[J]. 上海交通大学学报（医学版）, 2021 , 41(8) : 1099 -1102 . DOI: 10.3969/j.issn.1674-8115.2021.08.017

Abstract

Enamel matrix protein has been proved to promote periodontal regeneration, and later developed into enamel matrix derivative (EMD). At present, EMD has been used in clinic for more than 20 years, and a number of clinical studies have shown that it also has a prominent role in periodontal regeneration. Angiogenesis also plays an essential role in tissue regeneration. This process allows the transportation of numerous cytokines to tissue defect areas via newly formed blood vessel, which can then stimulate periodontal regeneration. Several researches have indicated that EMD can stimulate angiogenesis. In this paper, the effects of EMD on periodontal regeneration-related cells and angiogenesis are reviewed.

Key words： enamel matrix derivative (EMD); angiogenesis; vascular endothelial cell; periodontal regeneration

参考文献

1 Menicanin D, Hynes K, Han J, et al. Cementum and periodontal ligament regeneration[J]. Adv Exp Med Biol, 2015, 881: 207-236.
2 Nyman S, Lindhe J, Karring T, et al. New attachment following surgical treatment of human periodontal disease[J]. J Clin Periodontol, 1982, 9(4): 290-296.
3 Ivanovski S. Periodontal regeneration[J]. Aust Dent J, 2009, 54(): S118-S128.
4 Miron RJ, Sculean A, Cochran DL, et al. Twenty years of enamel matrix derivative: the past, the present and the future[J]. J Clin Periodontol, 2016, 43(8): 668-683.
5 Lindskog S. Formation of intermediate cementum. Ⅰ: early mineralization of aprismatic enamel and intermediate cementum in monkey[J]. J Craniofac Genet Dev Biol, 1982, 2(2): 147-160.
6 Slavkin HC, Bessem C, Fincham AG, et al. Human and mouse cementum proteins immunologically related to enamel proteins[J]. Biochim Biophys Acta, 1989, 991(1): 12-18.
7 Rathva VJ. Enamel matrix protein derivatives: role in periodontal regeneration[J]. Clin Cosmet Investig Dent, 2011, 3: 79-92.
8 王爽, 丰培勋, 陈悦, 等. 釉基质蛋白衍生物对人牙周膜干细胞分化、增殖的影响[J]. 中国组织工程研究, 2015, 19(23): 3716-3722.
9 温玉洁, 冯馨仪, 葛颂. 釉基质蛋白对牙及牙周组织再生的研究进展[J]. 2016, 26(6): 390-391.
10 陈发明, 高丽娜, 陈芳. 牙周再生治疗现状和进展[J]. 口腔疾病防治, 2019, 27(1): 9-16.
11 Tavelli L, McGuire MK, Zucchelli G, et al. Biologics-based regenerative technologies for periodontal soft tissue engineering[J]. J Periodontol, 2020, 91(2): 147-154.
12 Wennstr?m JL, Lindhe J. Some effects of enamel matrix proteins on wound healing in the dento-gingival region[J]. J Clin Periodontol, 2002, 29(1): 9-14.
13 Liu AQ, Hu CH, Jin F, et al. Contributions of bioactive molecules in stem cell-based periodontal regeneration[J]. Int J Mol Sci, 2018, 19(4): 1016.
14 吕雪雯, 潘春玲. 牙周组织工程的研究进展[J]. 中国医科大学学报, 2019, 48(12): 1132-1136.
15 董正谋, 刘锐, 刘鲁川, 等. 种子细胞在牙周组织再生治疗中的研究进展[J]. 国际口腔医学杂志, 2019, 46(1): 48-54.
16 韩佳吟, 孙坚炜, 雷利红, 等. 牙周膜再生研究进展[J]. 中国实用口腔科杂志, 2019, 12(5): 300-306.
17 Sakoda K, Nakajima Y, Noguchi K. Enamel matrix derivative induces production of vascular endothelial cell growth factor in human gingival fibroblasts[J]. Eur J Oral Sci, 2012, 120(6): 513-519.
18 Wang Y, Zhang Y, Jing D, et al. Enamel matrix derivative improves gingival fibroblast cell behavior cultured on titanium surfaces[J]. Clin Oral Investig, 2016, 20(4): 685-695.
19 Wyganowska-Swiatkowska M, Urbaniak P, Lipinski D, et al. Human gingival fibroblast response to enamel matrix derivative, porcine recombinant 21.3-kDa amelogenin and 5.3-kDa tyrosine-rich amelogenin peptide[J]. Hum Cell, 2017, 30(3): 181-191.
20 Miron RJ, Dard M, Weinreb M. Enamel matrix derivative, inflammation and soft tissue wound healing[J]. J Periodontal Res, 2015, 50(5): 555-569.
21 Qi Y, Feng W, Cai J, et al. Effects of conservatively treated diseased cementum with or without EMD on in vitro cementoblast differentiation and in vivo cementum-like tissue formation of human periodontal ligament cells[J]. Cell Prolif, 2014, 47(4): 310-317.
22 Villa O, Brookes SJ, Thiede B, et al. Subfractions of enamel matrix derivative differentially influence cytokine secretion from human oral fibroblasts[J]. J Tissue Eng, 2015, 6: 2041731415575857.
23 Heng NH, Zahlten J, Cordes V, et al. Effects of enamel matrix derivative and transforming growth factor-β1 on connective tissue growth factor in human periodontal ligament fibroblasts[J]. J Periodontol, 2015, 86(4): 569-577.
24 Liu J, Zhao Z, Ruan J, et al. Stem cells in the periodontal ligament differentiated into osteogenic, fibrogenic and cementogenic lineages for the regeneration of the periodontal complex[J]. J Dent, 2020, 92: 103259.
25 都沙沙, 蔡智国, 杨琨, 等. 牙源性间充质干细胞促进牙周组织再生的可能性与前景[J]. 中国组织工程研究, 2019, 23(17): 2782-2788.
26 Wang ZS, Feng ZH, Wu GF, et al. In vitro studies on human periodontal ligament stem cell sheets enhanced by enamel matrix derivative[J]. Colloids Surf B Biointerfaces, 2016, 141: 102-111.
27 Miron RJ, Chandad F, Buser D, et al. Effect of enamel matrix derivative liquid on osteoblast and periodontal ligament cell proliferation and differentiation[J]. J Periodontol, 2016, 87(1): 91-99.
28 Wyganowska-Swiatkowska M, Urbaniak P, Lipinski D, et al. Effects of enamel matrix proteins on adherence, proliferation and migration of epithelial cells: a real-time in vitro study[J]. Exp Ther Med, 2017, 13(1): 160-168.
29 Aspriello SD, Zizzi A, Spazzafumo L, et al. Effects of enamel matrix derivative on vascular endothelial growth factor expression and microvessel density in gingival tissues of periodontal pocket: a comparative study[J]. J Periodontol, 2011, 82(4): 606-612.
30 Amin HD, Olsen I, Knowles J, et al. A tyrosine-rich amelogenin peptide promotes neovasculogenesis in vitro and ex vivo[J]. Acta Biomater, 2014, 10(5): 1930-1939.
31 Andrukhov O, Gemperli AC, Tang Y, et al. Effect of different enamel matrix derivative proteins on behavior and differentiation of endothelial cells[J]. Dent Mater, 2015, 31(7): 822-832.
32 Jonke E, Gemperli AC, Zhang TW, et al. Effect of tyrosine-rich amelogenin peptide on behavior and differentiation of endothelial cells[J]. Clin Oral Investig, 2016, 20(8): 2275-2284.
33 Park JS, Pabst AM, Ackermann M, et al. Biofunctionalization of porcine-derived collagen matrix using enamel matrix derivative and platelet-rich fibrin: influence on mature endothelial cell characteristics in vitro[J]. Clin Oral Investig, 2018, 22(2): 909-917.
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