上海交通大学学报（医学版）

上海交通大学学报（医学版） >

2021 , Vol. 41 >Issue 10: 1290 - 1296

DOI: https://doi.org/10.3969/j.issn.1674-8115.2021.10.003

论著 · 基础研究

METTL10表达下调与足细胞损伤的关系

  • 包继文 ,
  • 李子扬 ,
  • 尧欢珍 ,
  • 吴蓓 ,
  • 周文彦 ,
  • 顾乐怡 ,
  • 王玲
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  • 上海交通大学医学院附属仁济医院肾脏内科，上海 200127
包继文（1995—），女，硕士生；电子信箱：615692239@qq.com。
王 玲，电子信箱：shwangling@126.com。

网络出版日期: 2021-09-22

基金资助

上海市自然科学基金(19ZR1430800)

收起

Relation of down-regulation of METTL10 and podocyte injury

  • Ji-wen BAO ,
  • Zi-yang LI ,
  • Huan-zhen YAO ,
  • Bei WU ,
  • Wen-yan ZHOU ,
  • Le-yi GU ,
  • Ling WANG
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  • Department of Nephrology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
WANG Ling, E-mail: shwangling@126.com.

Online published: 2021-09-22

Supported by

Natural Science Foundation of Shanghai(19ZR1430800)

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摘要

目的·分析足细胞损伤中的差异基因表达，并验证其中关键基因METTL10的蛋白表达。方法·从NCBI_GBO数据库下载足细胞损伤相关RNA芯片数据集（GSE108629），包括正常足细胞组（n=4）和损伤足细胞组（n=4）；采用limma 软件包和affy软件包进行2组间差异表达基因分析，以基因表达倍数变化（fold change，FC）对数的绝对值| log FC |≥2且校正P值（q值）<0.05为筛选条件。收集于上海交通大学医学院附属仁济医院进行肾组织活检并诊断为局灶节段性肾小球硬化或者微小病变的大量蛋白尿患者，以肾组织活检结果为轻微病变或者良性肾小动脉硬化症的患者为对照组；收集患者的基本资料和临床资料，利用免疫荧光染色检测患者肾脏病理标本中METTL10蛋白表达水平。BAL/BC小鼠随机分为2组：阿霉素（adriamycin，ADR）处理组按10 mg/kg尾静脉注射ADR构建小鼠肾病模型（n=6）；对照组等体积注射磷酸盐缓冲液（n=6）。利用代谢笼收集小鼠的24 h尿液，分别检测注射前和注射14 d时2组小鼠尿白蛋白肌酐比值（urinary albumin creatinine ratio，UACR）；在第15日处死小鼠，收集小鼠肾脏组织标本，通过蛋白印迹法检测ADR诱导小鼠肾病模型中METTL10和足细胞标志物包括synaptopodin、nephrin、podocin和Wilms’ tumor 1 （WT1）的表达水平。结果·在损伤足细胞中发现5 353个差异表达基因（3 402个显著上调，1 951个显著下调），METTL10是其中一个显著下调的基因。免疫荧光检测结果显示METTL10在人正常肾小球组织中表达，并且METTL10与足细胞标志物podocalyxin存在共定位；与对照组相比，METTL10在大量蛋白尿患者肾小球组织中表达明显下降。与对照组相比，注射14 d时ADR处理组小鼠UACR明显升高（P=0.005）；蛋白印迹法检测结果显示，与对照组小鼠相比，ADR处理组小鼠肾脏中METTL10表达下调（P=0.001），同时伴随足细胞标志物synaptopodin、nephrin、podocin和WT1表达下降，差异均具有统计学意义（对应P值分别为0.001、0.005、0.000和0.006）。结论·METTL10表达下调可能与足细胞损伤有关。

关键词： 足细胞损伤; 蛋白尿; METTL10; 差异表达基因

本文引用格式

包继文 , 李子扬 , 尧欢珍 , 吴蓓 , 周文彦 , 顾乐怡 , 王玲 . METTL10表达下调与足细胞损伤的关系[J]. 上海交通大学学报（医学版）, 2021 , 41(10) : 1290 -1296 . DOI: 10.3969/j.issn.1674-8115.2021.10.003

Abstract

Objective

·To identify the differentially expressed genes (DEGs) in podocyte injury and validate the expression of METTL10, one of these critical genes.

Methods

·The microarray expression profile of a podocyte injury model, dataset GSE108629, was downloaded from the GEO database, containing 4 normal podocyte samples and 4 injured podocyte samples, and the DEGs were diagnosed using the limma software package and affy software package (|log FC| ≥ 2 and adjusted P value<0.05). Patients with significant proteinuria who underwent renal biopsy and were diagnosed as focal segmental glomerulosclerosis or minimal change disease were retrospectively enrolled. Control group was patients who underwent renal biopsy and were proven as slight pathological changes or benign arteriolar neophrosclerosis. The general and clinical data were collected, and the expression of METTL10 in renal tissue was detected by immunofluorescence staining. Adriamycin (ADR)-induced nephropathy mouse model was established by a single intravenous injection of ADR into BAL/BC mouse. Mice were randomly divided into two groups: control group (treated with the same volume of PBS) and ADR group (treated with ADR 10 mg/kg body weight). On Day 0 and Day 14, urine was collected for 24 h using a metabolic cage and urinary albumin creatinine ratio (UACR) of mice were detected; on Day 15, mice were sacrificed and kidney tissue was collected. The expression of METTL10 and podocyte marker proteins like synaptopodin, nephrin, podocin, and Wilms' tumor 1 (WT1) in kidneys from ADR-treated mouse model was analyzed by Western blotting.

Results

·A total of 5 353 genes were significantly differentially expressed in injured podocytes compared with intact podocytes, among which 3 402 DEGs were up-regulated and 1 951 DEGs were down-regulated. METTL10 was found to be one of those that overe significantly down-regulated. Immunofluorescence staining indicated that METTL10 was expressed in normal human glomeruli and METTL10 was colocalized with podocalyxin, one of podocyte specific markers. Additionally, METTL10 was low expressed in the renal biopsy specimens from patients with significant proteinuria compared with that in the control group. On Day 14 after administration of ADR, mice developed significant proteinuria, shown by UACR detection (P=0.005); Western blotting revealed that METTL10 was significantly down-regulated (P=0.001), along with the significantly decreased expression of synaptopodin (P=0.001), nephrin (P=0.005), podocin (P=0.000) and WT1 (P=0.006) in ADR-treated mice compared with that in normal mice. The differences of the expression of METTL10 and the above podocyte markers between these two groups were statistically significant.

Conclusion

·The down-regulation of METTL10 may be involved in podocyte injury.

Key words： podocyte injury; proteinuria; METTL10; differentially expressed genes (DEGs)

参考文献

1 Eckardt K U, Coresh J, Devuyst O, et al. Evolving importance of kidney disease: from subspecialty to global health burden[J]. Lancet, 2013, 382(9887): 158-169.
2 Guo Y, Pace J, Li Z, et al. Podocyte-specific induction of Krüppel-like factor 15 restores differentiation markers and attenuates kidney injury in proteinuric kidney disease[J]. J Am Soc Nephrol, 2018, 29(10): 2529-2545.
3 Zhou LT, Cao YH, Lv LL, et al. Feature selection and classification of urinary mRNA microarray data by iterative random forest to diagnose renal fibrosis: a two-stage study[J]. Sci Rep, 2017, 7: 39832.
4 Cechova S, Dong F, Chan F, et al. MYH9 E1841K mutation augments proteinuria and podocyte injury and migration[J]. J Am Soc Nephrol, 2018,29(1): 155-167.
5 Yamamoto-Nonaka K, Koike M, Asanuma K, et al. Cathepsin D in podocytes is important in the pathogenesis of proteinuria and CKD[J]. J Am Soc Nephrol, 2016, 27(9): 2685-2700.
6 Kriz W, Lemley KV. A potential role for mechanical forces in the detachment of podocytes and the progression of CKD[J]. J Am Soc Nephrol, 2015, 26(2): 258-269.
7 Nagata M. Podocyte injury and its consequences[J]. Kidney Int, 2016, 89(6): 1221-1230.
8 Perico L, Conti S, Benigni A, et al. Podocyte-actin dynamics in health and disease[J]. Nat Rev Nephrol, 2016, 12(11): 692-710.
9 Blaine J, Dylewski J. Regulation of the actin cytoskeleton in podocytes[J]. Cells, 2020, 9(7): 1700.
10 Torban E, Braun F, Wanner N, et al. From podocyte biology to novel cures for glomerular disease[J]. Kidney Int, 2019, 96(4): 850-861.
11 Yu SM, Nissaisorakarn P, Husain I, et al. Proteinuric kidney diseases: a podocyte's slit diaphragm and cytoskeleton approach[J]. Front Med (Lausanne), 2018, 5: 221.
12 Wang WN, Zhang WL, Zhou GY, et al. Prediction of the molecular mechanisms and potential therapeutic targets for diabetic nephropathy by bioinformatics methods[J]. Int J Mol Med, 2016, 37(5): 1181-1188.
13 Butz H, Szabó PM, Nofech-Mozes R, et al. Integrative bioinformatics analysis reveals new prognostic biomarkers of clear cell renal cell carcinoma[J]. Clin Chem, 2014, 60(10): 1314-1326.
14 Lay AC, Barrington AF, Hurcombe JA, et al. A role for NPY-NPY2R signaling in albuminuric kidney disease[J]. Proc Natl Acad Sci U S A, 2020,117(27): 15862-15873.
15 Falnes P?, Jakobsson ME, Davydova E, et al. Protein lysine methylation by seven-β-strand methyltransferases[J]. Biochem J, 2016, 473(14): 1995-2009.
16 Li Z, Gonzalez PA, Sasvari Z, et al. Methylation of translation elongation factor 1A by the METTL10-like See1 methyltransferase facilitates tombusvirus replication in yeast and plants[J]. Virology, 2014, 448: 43-54.
17 Lipson RS, Webb KJ, Clarke SG. Two novel methyltransferases acting upon eukaryotic elongation factor 1A in Saccharomyces cerevisiae[J]. Arch Biochem Biophys, 2010, 500(2): 137-143.
18 Hellwege JN, Velez ED, Giri A, et al. Mapping eGFR loci to the renal transcriptome and phenome in the VA Million Veteran Program[J]. Nat Commun, 2019, 10(1): 3842.
19 Rane MJ, Zhao Y, Cai L. Kr?ppel-like factors (KLFs) in renal physiology and disease[J]. EBioMedicine, 2019, 40: 743-750.
20 卢甜美, 杜玄一. 足细胞损伤机制研究进展[J]. 现代医学, 2017,45(2):311-314.
21 Zhou LT, Qiu S, Lv LL, et al. Integrative bioinformatics analysis provides insight into the molecular mechanisms of chronic kidney disease[J]. Kidney Blood Press Res, 2018, 43(2): 568-581.
22 尚懿纯, 曹式丽, 窦一田, 等. 局灶节段性肾小球硬化足细胞损伤生物标志物的研究进展[J]. 安徽医科大学学报, 2017,52(1):151-154.
23 Jefferson JA, Shankland SJ. The pathogenesis of focal segmental glomerulosclerosis[J]. Adv Chronic Kidney Dis, 2014, 21(5): 408-416.
24 Liapis H, Romagnani P, Anders HJ. New insights into the pathology of podocyte loss: mitotic catastrophe[J]. Am J Pathol, 2013, 183(5): 1364-1374.
25 Garg P. A review of podocyte biology[J]. Am J Nephrol, 2018,47:3-13.
26 Akram Z, Ahmed I, Mack H, et al. Yeast as a model to understand actin-mediated cellular functions in mammals-illustrated with four actin cytoskeleton proteins[J]. Cells, 2020, 9(3): 672.
27 Snape N, Li D, Wei T, et al. The eukaryotic translation elongation factor 1A regulation of actin stress fibers is important for infectious RSV production[J]. Virol J, 2018,15(1):182.
28 Gross SR. Actin binding proteins: their ups and downs in metastatic life[J]. Cell Adh Migr, 2013, 7(2): 199-213.
29 Li F, Mao X, Zhuang Q, et al. Inhibiting 4E-BP1 re-activation represses podocyte cell cycle re-entry and apoptosis induced by adriamycin[J]. Cell Death Dis, 2019, 10(3): 241.
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