上海交通大学学报（医学版）

上海交通大学学报（医学版） >

2023 , Vol. 43 >Issue 5: 600 - 605

DOI: https://doi.org/10.3969/j.issn.1674-8115.2023.05.010

论著 · 临床研究

伴阿尔茨海默病牙周炎患者血清Th17/Treg相关细胞因子水平变化研究

  • 谢欣宜 ,
  • 周薇 ,
  • 邱澈 ,
  • 沈慧 ,
  • 宋忠臣
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  • 上海交通大学医学院附属第九人民医院牙周病科，上海交通大学口腔医学院，国家口腔医学中心，国家口腔疾病临床医学研究中心，上海市口腔医学重点实验室，上海市口腔医学研究所，上海 200011
谢欣宜（1996—），女，硕士生；电子信箱：honeymelon2015@sjtu.edu.cn。
宋忠臣，电子信箱：szhongchen@sina.com。

收稿日期: 2022-11-22

  录用日期: 2023-04-28

  网络出版日期: 2023-07-11

基金资助

国家自然科学基金(82071112);上海申康医院发展中心临床创新三年行动计划资助(SHDC2020CR5015)

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Changes in serum Th17/Treg-related cytokine levels in periodontitis patients with Alzheimer′s disease

  • Xinyi XIE ,
  • Wei ZHOU ,
  • Che QIU ,
  • Hui SHEN ,
  • Zhongchen SONG
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  • Department of Periodontology, Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai 200011, China
SONG Zhongchen, E-mail: szhongchen@sina.com.

Received date: 2022-11-22

  Accepted date: 2023-04-28

  Online published: 2023-07-11

Supported by

National Natural Science Foundation of China(82071112);Clinical Research Plan of SHDC(SHDC2020CR5015)

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摘要

目的·观察血清Th17/Treg水平的变化对阿尔茨海默病与牙周炎的影响。方法·纳入2020年6月—2021年12月就诊于上海交通大学医学院附属精神卫生中心老年科共18位伴阿尔茨海默病的牙周炎患者与同时期就诊于上海交通大学医学院附属第九人民医院共15位认知功能正常的牙周炎患者，检查并记录患者探诊深度（probing depth，PD）、附着丧失（clinical attachment loss，CAL）及探诊出血位点比例（percentage of bleeding on probing，BOP%）。使用酶联免疫吸附法检测血清中Th17相关细胞因子白细胞介素-6（interleukin-6，IL-6）、IL-17、IL-22及Treg相关细胞因子IL-4、IL-10、转化生长因子-β（transforming growth factor-β，TGF-β）水平。采用样本独立t检验比较2组牙周指标与细胞因子水平的差异，P<0.05表示差异具有统计学意义。结果·伴阿尔茨海默病的牙周炎患者PD（P=0.021）、CAL（P=0.023）及BOP%（P=0.018）均显著高于认知功能正常的牙周炎患者。伴阿尔茨海默病的牙周炎患者血清IL-6（P=0.010）、IL-17（P=0.022）、IL-22（P=0.031）水平均显著高于认知功能正常的牙周炎患者；同时，组中IL-4（P=0.002）及IL-10（P=0.049）、TGF-β（P=0.006）水平相较于认知功能正常的牙周炎患者显著降低。结论·伴阿尔茨海默病牙周炎患者的牙周状况比认知功能正常的牙周炎患者差，Th17相关促炎细胞因子升高，而Treg相关抗炎因子降低，说明牙周炎可能通过介导Th17/Treg相关的慢性炎症反应影响阿尔茨海默病的发生发展。

关键词： 阿尔茨海默病; 牙周炎; 细胞因子; Th17细胞; Treg细胞

本文引用格式

谢欣宜 , 周薇 , 邱澈 , 沈慧 , 宋忠臣 . 伴阿尔茨海默病牙周炎患者血清Th17/Treg相关细胞因子水平变化研究[J]. 上海交通大学学报（医学版）, 2023 , 43(5) : 600 -605 . DOI: 10.3969/j.issn.1674-8115.2023.05.010

Abstract

Objective ·To observe the role of serum Th17/Treg levels in the correlation between Alzheimer′s disease and periodontitis. Methods ·Eighteen periodontitis patients with Alzheimer′s disease who visited the Department of Geriatric, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, and 15 periodontitis patients with normal cognitive function who visited the Department of Periodontology, Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine from June 2020 to December 2021, were enrolled in this study. Probing depth (PD), clinical attachment loss (CAL) and percentage of bleeding on probing (BOP%) were examined and recorded. Serum levels of Th17-related cytokines interleukin-6 (IL-6), IL-17, and IL-22, Treg-related cytokines IL-4 and IL-10, and transforming growth factor-β (TGF-β) were measured by ELISA. The differences of periodontal status and cytokine levels were compared by using t-test. The difference was statistically significant when P<0.05. Results ·The PD (P=0.021), CAL (P=0.018) and BOP% (P=0.018) were significantly higher in the periodontitis patients with Alzheimer′s disease than those in periodontitis patients with normal cognitive function. The serum IL-6 (P=0.010), IL-17 (P=0.022) and IL-22 (P=0.031) levels were significantly higher in the periodontitis patients with Alzheimer′s disease than those in periodontitis patients with normal cognitive function, while the levels of IL-10 (P=0.049), IL-4 (P=0.002) and TGF-β (P=0.006) in the periodontitis patients with Alzheimer′s disease were significantly lower compared with those in periodontitis patients with normal cognitive function. Conclusion ·The periodontal condition of periodontitis patients with Alzheimer′s disease is worse compared to that of periodontitis patients with normal cognitive function. The expression of Th17-related pro-inflammatory cytokines is elevated, while the expression of Treg-related anti-inflammatory factors is decreased. Thus, periodontitis may affect the development of Alzheimer′s disease by mediating Th17/Treg-related chronic inflammatory responses.

Key words： Alzheimer′s disease; periodontal disease; cytokins; Th17 cell; Treg cell

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