目的 探讨14-3-3ζ蛋白在胃癌和正常胃黏膜组织中的表达及其临床意义。方法 选取100例手术切除的胃癌组织标本及其中76例癌旁正常胃黏膜组织标本制备组织芯片，应用免疫组织化学方法检测组织芯片中14-3-3ζ蛋白的表达。分析胃癌患者的临床病理学特征与14-3-3ζ蛋白表达的相关性。结果 100例胃癌组织中79例(79.00%)表达14-3-3ζ蛋白；而正常胃黏膜中仅散在个别细胞弱表达。按组织学分类法，在乳头状腺癌和管状腺癌中14-3-3ζ蛋白阳性表达率分别为100%和86.75%，显著高于黏液腺癌和印戒细胞癌（42.86%、0）（P<0.05）。按Lauren分类法，肠型胃癌中14-3-3ζ蛋白阳性表达率显著高于弥漫型胃癌(90.91% vs 73.13%)（P<0.05）。在不同浸润深度的胃癌中，肌层和浆膜层的14-3-3ζ蛋白阳性表达率分别为78.57%和81.93%，均显著高于黏膜层和黏膜下层（0、0）（P<0.05）。进展期胃癌的14-3-3ζ蛋白阳性表达率为81.44%，而3例早期胃癌均不表达（P<0.05）。结论 胃癌组织中14-3-3ζ蛋白表达与其组织学类型和浸润状况密切相关。提示14-3-3ζ蛋白有可能成为评估胃癌的预后指标和潜在的生物治疗靶点。

Objective To investigate the expression of 14-3-3ζ protein in gastric cancer and its clinical significance. Methods Tissue microarray technique and immunohistochemical staining were used to investigate the expression of 14-3-3ζ protein in 100 cases of gastric cancer tissues and 76 cases of para-cancer gastric mucosa tissues. The correlation between expression of 14-3-3ζ protein and clinicopathologic characteristics of patients with gastric cancer were analysed.ResultsFor 100 cases of gastric cancer tissues, 79 (79.00%) expressed 14-3-3ζ protein, while few cells expressed 14-3-3ζ protein in normal gastric mucosa. The positive expression of 14-3-3ζ protein in papillary adenocarcinoma (100%) and tubular adenocarcinoma (86.75%) was significantly higher than those in mucinous adenocarcinoma (42.86%) and signet-ring cell carcinoma (0) (P<0.05). By Lauren's classification, the positive expression of 14-3-3ζ protein in intestinal type gastric cancer was significantly higher than that in diffuse type gastric cancer (90.91% vs 73.13%)（P<0.05）. By infiltration degree of gastric cancer, the positive expression of 14-3-3ζ protein in muscular layer (78.57%) and serosa layer (81.93%) was significantly higher than that in mucosa layer (0) and submucosa layer (0) (P<0.05）. The positive expression of 14-3-3ζ protein in advanced gastric cancer was 81.44%, while 14-3-3ζ protein was not expressed in all the 3 cases of early gastric cancer （P<0.05）.  Conclusion The expression of 14-3-3ζ protein is significantly correlated with the histological classification and infiltration degree of gastric cancer tissues. 14-3-3ζ protein may be used as a marker for prognosis evaluation and as a potential target of biotherapy for gastric cancer.