目的 构建DLX4酵母双杂交系统并鉴定其自激活作用。方法 构建人胎盘酵母双杂交cDNA文库，并行PCR鉴定；构建3条DLX4诱饵质粒，包括pDEST32-DLX4(4-1C)、pDEST32-DLX4(4-1N)和pDEST32-DLX4(4-2)；将DLX4诱饵质粒转入酵母MaV203菌株，并检测其在酵母细胞中有无自激活作用。结果 PCR证实成功构建了人胎盘酵母双杂交文库；3条DLX4诱饵质粒中，pDEST32-DLX4(4-2)和pDEST32-DLX4(4-1C)没有自激活作用，可用于文库筛选。结论 成功构建了酵母双杂交文库和DLX4诱饵质粒，可用于筛选与DLX4相互作用的蛋白。

Objective To construct a yeast-two hybrid system of DLX4 and identify its self-activation. Methods Human placenta yeast-two cDNA bank was constructed, and was identified by PCR. DLX4 bait plasmids including pDEST32-DLX4(4-1C), pDEST32-DLX4(4-1N) and pDEST32-DLX4(4-2) were constructed and transfected into yeast MaV203 cells, and the self-activation effect was detected. Results It was verified by PCR that human placental cDNA bank was successfully constructed. pDEST32-DLX4(4-2) and pDEST32-DLX4(4-1C) had no self-activation effect, and could be used in the yeast two-hybrid system. Conclusion Yeast-two hybrid bank and DLX4 bait plasmids have been successfully constructed, and can be used to screen the protein interacting with DLX4.