论著(基础研究)

口腔鳞状细胞癌细胞TLR3表达及其配体poly(I:C)的诱导凋亡作用

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  • 上海交通大学 医学院附属第九人民医院 1.检验科, 2.上海市口腔医学重点实验室, 上海 200011
罗清琼(1985—), 女, 硕士;电子信箱: luoqingqiong2007@126.com。

网络出版日期: 2011-02-01

基金资助

上海市浦江人才计划(09PJ1407100);教育部留学回国人员科研启动基金(20091001);上海市自然科学基金(08ZR1412700);上海市重点学科开放基金(S30206)

Expression of TLR3 in oral squamous carcinoma cells and induction of apoptosis by its ligand poly(I:C)

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  • 1.Department of Laboratory, 2.Shanghai Key Laboratory of Stomatology, The Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China

Online published: 2011-02-01

Supported by

Shanghai Pujiang Talent Program, 09PJ1407100;Foundation for Returned Overseas from The Ministry of Education, 20091001;Shanghai Natural Science Foundation, 08ZR1412700;Shanghai Key Discipline Foundation, S30206

摘要

目的 研究口腔鳞状细胞癌细胞中Toll样受体3(TLR3)的表达及其配体poly (I:C)的生物学作用。方法 RT-PCR和流式细胞术检测口腔鳞状细胞癌细胞系CAL-27和HB中TLR3 mRNA和蛋白表达。以100 μg/mL poly(I:C)处理CAL-27, 分别于刺激后24、48和72 h时点采用MTT比色法测定细胞活力,24 h时点采用流式细胞术检测细胞凋亡;并设立空白对照。结果 CAL-27和HB中TLR3 mRNA和蛋白均呈高表达。CAL-27经poly(I:C)处理后,各检测时点的细胞活力均显著低于空白对照细胞(P<0.05);细胞凋亡率明显高于空白对照细胞[(16.67±1.202)% vs (7.00±1.155)%](P<0.05)。结论 口腔鳞状细胞癌细胞内高表达TLR3,其配体poly(I:C)能诱导口腔鳞状细胞癌细胞凋亡。

本文引用格式

罗清琼, 陈万涛, 胡水清, 等 . 口腔鳞状细胞癌细胞TLR3表达及其配体poly(I:C)的诱导凋亡作用[J]. 上海交通大学学报(医学版), 2011 , 31(1) : 5 . DOI: 10.3969/j.issn.1674-8115.2011.01.002

Abstract

Objective To investigate the expression of Toll-like receptor 3 (TLR3) and the biological characteristics of its ligand poly (I:C) in oral squamous carcinoma cells. Methods The expression of TLR3 mRNA and protein in oral squamous carcinoma CAL-27 cells and HB cells was detected by RT-PCR and flow cytometry. CAL-27 cells were treated with 100 μg/mL poly (I:C), cell viability was determined by MTT assay at 24, 48 and 72 h after stimulation, and cell apoptosis was detected by flow cytometry. Besides, blank controls were established. Results TLR3 mRNA and protein were highly expressed in CAL-27 and HB cells. After treatment by poly (I:C), the viability of CAL-27 cells was significantly lower than that of blank controls at each time point (P<0.05), and the apoptosis rate of CAL-27 cells was significantly higher than that of blank controls [(16.67±1.202)% vs (7.00±1.155)%](P<0.05). Conclusion TLR3 is highly expressed in oral squamous carcinoma cells, and its ligand poly(I:C) can induce the apoptosis of oral squamous carcinoma cells.

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