飞秒激光切割大鼠膀胱上皮组织的体外实验
网络出版日期: 2011-07-27
基金资助
国家自然科学基金(30801154/c160606)
In vitro study of ablation of rat bladder epithelial tissues by femtosecond laser
Online published: 2011-07-27
Supported by
National Natural Science Foundation of China, 30801154/c160606
潘玉龙, 陈 方, 康 健, 等 . 飞秒激光切割大鼠膀胱上皮组织的体外实验[J]. 上海交通大学学报(医学版), 2011 , 31(7) : 918 . DOI: 10.3969/j.issn.1674-8115.2011.07.011
Objective To observe the efficiency and range of collateral tissue damage in ablation of rat bladder epithelial tissues by femtosecond laser. Methods Bladder epithelial tissues of SD rats were ablated by femtosecond laser with different working powers (1, 2, 5, 10 and 20 times of threshold power and equivalent frequencies). Sections with HE staining were prepared for the ablated tissues. The depth and range of collateral tissue damage of bladder epithelial tissues ablated by femtosecond laser were observed by light microscopy, and the ablation efficiency was calculated. Results When the working powers of femtosecond laser were 5 to 20 times of threshold power and the equivalent frequencies were 62.5 Hz to 1 000 Hz, the ablation efficiency varied from 10 to 120 μm/s and the collateral tissue damage ranged from 10 to 100 μm. Conclusion Femtosecond laser can precisely ablate the rat bladder epithelial tissues with minimal range of collateral tissue damage, and may be a potential alternative for treatment of urinary stricture.
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