目的 探讨葛根总黄酮（PRF）联合三氧化二砷（ATO）对M2型急性髓系白血病细胞株Kasumi-1和HL-60细胞增殖和凋亡的影响。方法 以100 μg/mL PRF和1 μmol/L ATO联合处理Kasumi-1和HL-60细胞48 h（RRF+ATO组），MTT法检测细胞增殖抑制率，Annexin V-FITC/PI双染法流式细胞术检测细胞早期凋亡率，Real-Time PCR检测凋亡抑制基因Bcl-2、Survivin mRNA表达，Western blotting检测细胞凋亡相关蛋白酶Caspase-3、-8、-9的表达。设立单用100 μg/mL PRF的PRF处理组和空白对照组。结果 RRF+ATO组Kasum-1细胞增殖抑制率、早期凋亡率以及Caspase-3、-9表达均显著高于PRF处理组（P<0.05），细胞中Bcl-2 mRNA表达的下调趋势明显。RRF+ATO组HL-60细胞各项指标检测结果与PRF处理组比较差异均无统计学意义(P>0.05)。结论 PRF联合ATO能有效抑制Kasumi-1细胞增殖并诱导早期凋亡，而对HL-60细胞则无明显影响。

Objective To investigate the effects of co-treatment with puerariae radix flavones (PRF) and arsenic trioxide (ATO) on proliferation and apoptosis of Kasumi-1 cells and HL-60 cells of acute myeloid leukemia M2. Methods Kasumi-1 cells and HL-60 cells were treated with 100 μg/mL PRF and 1 μmol/L ATO for 48 h (RRF+ATO group). MTT assay was employed to measure the inhibition rate of cell proliferation, Annexin V-FITC/PI double staining was used to determine the early apoptosis rate by flow cytometry, the expression of Bcl-2 and Survivin mRNA was detected by Real-Time PCR, and the expression of Caspase-3, Caspase-8 and Caspase-9 protein was detected by Western blotting. Cells only treated with 100 μg/mL PRF were served as PRF group, and blank control group was also established. Results The inhibition rate of cell proliferation, early apoptosis rate and expression of Caspase-3 and Caspase-9 protein of Kasumi-1 cells in RRF+ATO group were significantly higher than those in PRF group (P<0.05), and there was an obvious trend of down-regulation of expression of Bcl-2 mRNA in Kasumi-1 cells. There was no significant difference in each parameter of HL-60 cells between RRF+ATO group and PRF group (P>0.05). Conclusion Co-treatment with PRF and ATO can effectively inhibit the proliferation and induce early apoptosis of Kasumi-1 cells, while that has no significant effect on HL-60 cells.