慢性应激大鼠脑源性营养因子的表达分析
网络出版日期: 2012-04-27
基金资助
国家自然科学基金(81101007);上海市科委基金(09411965600)
Analysis of expression of brain-derived neurotrophic factor in rats with chronic unpredictable mild stress
Online published: 2012-04-27
Supported by
National Natural Science Foundation of China, 81101007;Shanghai Science and Technology Committee Foundation, 09411965600
目的 探讨慢性不可预知轻度应激(CUMS)大鼠模型各脑区脑源性神经营养因子(BDNF) 的表达及抗抑郁药对BDNF表达的影响。方法 将24只雄性SD大鼠随机分为三组(n=8):A组为对照组;B组为CUMS应激组;C组为CUMS应激+西太普兰用药组(每天腹腔注射西酞普兰水溶液2 mL,10 mg/kg)。实验为期6周,每周称量大鼠体质量,每3周测试大鼠的糖水偏爱度,造模前及造模6周末通过旷场试验评价大鼠行为。6周后处死大鼠获取脑组织,采用Real-Time PCR检测各脑区BDNF mRNA表达水平。结果 第6周末,B组大鼠的糖水偏爱度明显低于A、C两组(P<0.05)且行为增多。应激实验开始后,B、C两组大鼠体质量均明显低于A组(P<0.05)。B组BDNF mRNA在海马中的表达高于A组,但差异无统计学意义(P=0.07);C组纹状体中BDNF mRNA表达明显低于A组(P<0.05)。结论 慢性应激可能引起海马BDNF表达升高,抗抑郁药物可能导致纹状体BDNF表达降低。
关键词: 慢性不可预知轻度应激; 抑郁症; 脑源性神经营养因子
王静华, 肖泽萍, 肖世富, 等 . 慢性应激大鼠脑源性营养因子的表达分析[J]. 上海交通大学学报(医学版), 2012 , 32(4) : 440 . DOI: 10.3969/j.issn.1674-8115.2012.04.016
Objective To investigate the expression of brain-derived neurotrophic factor (BDNF) in different areas of brain in rats with chronic unpredictable mild stress (CUMS), and explore the effects of antidepressant on expression of BDNF. Methods Twenty-four male SD rats were randomly assighed to control group (group A), CUMS group (group B) and CUMS + citalpram group (intraperitoneal injection of citalpram 2 mL once a day, 10 mg/kg) (group C), with 8 rats in each group. The experiment lasted for 6 weeks. Body weight was measured each week, sugar preference was evaluated every 3 weeks, and behavior of rats was assessed through open field tests before model establishment and 6 weeks after model establishment. Animals were sacrificed after 6 weeks, brain tissues were obtained, and Real-Time PCR was employed to detect the expression of BDNF mRNA in different areas of brain. Results At the end of the sixth week, rats in group B had significantly lower sugar preference than group A and group C (P<0.05), and had more behavior. After the beginning of stress experiment, the body weight of rats in group B and group C was significantly lower than that in group A (P<0.05). The expression of BDNF mRNA in hippocampus of rats in group B was higher than that in group A, while there was no significant difference between them (P=0.07). The expression of BDNF mRNA in striatum of rats in group C was significantly lower than that in group A (P<0.05). Conclusion Chronic stress may increase the expression of BDNF in hippocampus, while antidepressant may decrease the expression of BDNF in striatum.
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