论著(临床研究)

ApoA-Ⅰ在多囊卵巢综合征患者卵巢颗粒细胞中的表达

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  • 重庆医科大学附属第一医院辅助生育中心, 重庆 400016
仝腊娟(1986—), 女, 硕士生; 电子信箱: tonglajuan@yahoo.cn。

网络出版日期: 2013-05-28

Expression of ApoA-Ⅰ in granulosa cells in patients with polycystic ovary syndrome

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  • Department of Assistant Reproductive Centre, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China

Online published: 2013-05-28

摘要

目的 分析多囊卵巢综合征(PCOS)患者的卵巢颗粒细胞(GCs) 中载脂蛋白A-Ⅰ(ApoA-Ⅰ)的表达情况。方法 收集接受体外授精-胚胎移植治疗的20例PCOS患者(PCOS组)和20例对照组妇女的GCs和术前检查的血清。采用Western blotting技术检测GCs中ApoA-Ⅰ蛋白表达;采用Real-time PCR技术检测GCs中ApoA-ⅠmRNA表达;采用免疫比浊法检测血清ApoA-Ⅰ水平。结果 PCOS组GCs中ApoA-Ⅰ蛋白及mRNA的表达均显著低于对照组(P<0.001,P<0.01),PCOS组血清ApoA-Ⅰ水平亦显著低于对照组(P<0.05)。结论 PCOS患者GCs中ApoA-Ⅰ表达下调。

本文引用格式

仝腊娟, 丘 彦, 孟江萍, 等 . ApoA-Ⅰ在多囊卵巢综合征患者卵巢颗粒细胞中的表达[J]. 上海交通大学学报(医学版), 2013 , 33(5) : 655 . DOI: 10.3969/j.issn.1674-8115.2013.05.029

Abstract

Objective To investigate the expression of apolipoprotein A-Ⅰ (ApoA-Ⅰ) in granulosa cells (GCs) of patients with polycystic ovary syndrome (PCOS). Methods Forty women undergoing in vitro fertilization and embryo transfer were divided into PCOS group (n=20) and control group (n=20), and serum samples on preoperative examination day and GCs samples were collected. The expression of ApoA-Ⅰ protein in GCs was detected by Western blotting, the expression of ApoA-Ⅰ mRNA in GCs was determined by Real-time PCR, and the serum level of ApoA-Ⅰ was measured by immunoturbidimetry. Results The expression of ApoA-Ⅰ protein and mRNA in GCs in PCOS group was significantly lower than that in control group (P<0.001, P<0.01), and the serum level of ApoA-Ⅰ in PCOS group was also significantly lower than that in control group (P<0.05). Conclusion The expression of ApoA-Ⅰ is down-regulated in GCs in patients with PCOS.

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