›› 2011, Vol. 31 ›› Issue (5): 567-.doi: 10.3969/j.issn.1674-8115.2011.05.009

• Original article (Basic research) • Previous Articles     Next Articles

Expression of SSeCKS in renal tissues of rats with renal injury induced by AngⅡ

WANG Tao, WANG Ying-deng, ZHANG Wei   

  1. Department of Nephrology, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2011-05-28 Published:2011-05-27
  • Supported by:

    Shanghai Natural Science Foundation, 09ZR1417400

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Abstract:

Objective To establish the rat models of renal injury induced by angiotensionⅡ (AngⅡ), and investigate the expression of Src-suppressed C kinase substrate (SSeCKS) in the injured renal tissues. Methods A total of 26 rats were randomly divided into model group (n=10), sham operation group (n=10) and normal control group (n=6). Rats in model group and sham operation group were administered with AngⅡ and normal saline respectively via subcutaneous osmotic minipumps at the velocity of 200 ng/(kg·min), and those in control group did not receive any treatment. Twenty-four hour urine samples were collected on the third day after minipump implant, then rats were sacrificed, and renal cortical tissues were obtained. Sulfosalicylic acid method was adopted to quantify 24 h urinary protein, the ultrastructure of renal tissues were observed by transmission electron microscopy, the location of SSeCKS in renal tissues and fluorescence intensity were determined and analysed by immunofluorescence staining method, and the expression of SSeCKS mRNA in renal tissues was detected by RT-PCR. Results The quantity of 24 h urinary protein in model group was significantly higher than those in sham operation group and normal control group (P<0.05). Shrinkage and detachment of glomerular endothelial cells in model group were observed by transmission electron microscopy. Immunofluorescence staining revealed that SSeCKS mainly localized within glomerular basement and some mesangial cells, and fluorescence intensity of renal tissues in model group was significantly higher than that in sham operation group and normal control group. The expression of SSeCKS mRNA in renal tissues in model group was significantly higher than that in sham operation group and normal control group. Conclusion Rat models of renal injury have been successfully established by subcutaneous implant of osmotic minipump with AngⅡ, and the expression of SSeCKS may be significantly increased in the injured renal tissues.

Key words: renal injury, angiotensionⅡ, Src-suppressed C kinase substrateSrc