›› 2011, Vol. 31 ›› Issue (7): 942-.doi: 10.3969/j.issn.1674-8115.2011.07.016

• Original article (Clinical research) • Previous Articles     Next Articles

Relationship between DNA methylation of p73 gene and acute leukemia of children

ZHANG Yan1, GAO Yu1, WANG Xiao-jin2, ZHU Sha3, SHI Rong1, JIN Ping1, YANG You3, TIAN Ying1,3   

  1. 1.School of Public Health, Shanghai Jiaotong University, Shanghai 200025, China；2.Department of Biostatistics, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China；3.Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Online:2011-07-28 Published:2011-07-27
  • Supported by:

    National Natural Science Foundation of China, 30801345, 30901171；Shanghai Natural Science Foundation, 09ZR1416500；Shanghai Jiaotong University School of Medicine Foundation, 09XJ21054

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Abstract:

Objective To investigate the relationship between DNA methylation of p73 gene and acute leukemia of children. Methods One hundred and forty-two children with newly diagnosed acute leukemia aged less than 15 years were selected as case group, among whom 112 were acute lymphoblastic leukemia (ALL) and 30 were acute myeloid leukemia (AML). Another 123 sex and age-matched children undergoing physical examinations, outpatient visit or treatment without hematological diseases and tumors were served as control group. The methylation status of p73 gene of children in case group and control group was analysed by methylation-specific polymerase chain reaction (MSP). One sample of DNA methylated fragment and one sample of DNA non-methylated fragment of p73 gene were randomly selected for sequencing analysis of PCR product. Results The prevalence of DNA methylation of p73 gene in case group was significantly higher than that in control group （7.7% vs 0.8%, P<0.05）, while there was no significant difference in the deletion rate of p73 gene between case group and control group (6.3% vs 4.1%, P>0.05). The prevalence of DNA methylation of p73 gene in children with ALL in case group (8.9%) was significantly higher than that in control group (P<0.01). The sequencing results of two randomly selected samples were consistent with results of MSP. Conclusion DNA methylation of p73 gene may play an important role in the development of acute leukemia of children, especially in the development of ALL.

Key words: p73, acute leukemia, child, DNA methylationp