Abstract:

Objective To study the effects of inhibiting the lysine specific demethylase 1(LSD1) enzyme activity in hepatitis B virus (HBV) model mouse. Methods The BALB/C mice were injected recombinant plasmid HBV1.3 via tail vein to establish HBV mouse model. The female BALB/C mice were randomly divided into normal control group, model group, LSD1 small interfering RNA (siRNA) treatment group, and tranylcypromine (TCP) treatment group. The expressions of hepatitis B surface antigen (HBsAg) and HBV DNA in the peripheral blood of all mice were quantitatively detected by enzymelinked immuno sorbent assay, chemical luminescent method, and RT-PCR. The distribution of HBsAg in liver was detected by immunohistochemistry. The expression of LSD1 in splenic lymphocytes was detected by Western blotting. Results HBV mouse model was successfully established. Compared to model group, expressions of HBsAg, HBV DNA, and LSD1 were significantly lower in LSD1 siRNA treatment group and TCP treatment group (P＜0.01). And the distribution of HBsAg in liver also decreased remarkably. Conclusion Inhibiting the LSD1 enzyme activity plays a key role in elimination of HBV in HBV model mouse.

Key words: lysine specific demethylase 1, small interfering RNA, hepatitis B virus, hepatitis B surface antigen