›› 2018, Vol. 38 ›› Issue (3): 353-.doi: 10.3969/j.issn.1674-8115.2018.03.021

• Review • Previous Articles     Next Articles

Photochemical internalization and its application in gene delivery

LU Xue-min1 , LIU Li1#, XU Hong2#, ZHANG Lin-na2   

  1. 1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China; 2. Department of Gynecology, International Peace Maternal & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
  • Online:2018-03-28 Published:2018-05-03
  • Supported by:
    Biomedical Engineering Cross Research Foundation of Shanghai Jiao Tong University, YG2015MS41

Abstract: The translation of gene therapy from bench to bedside depends on efficient intracellular gene delivery. The macromolecular biologics such as gene combined with vectors tend to enter into the cells by means of endocytosis, where the biologics may encounter the risk of degradation in endolysosome. Recently, photochemical internalization (PCI) has emerged as a promising technique to overcome endo-lysosomal sequestration, which utilizes photosensitizer and light resulting in reactive oxygen species at sub-lethal level to destruct biofilm and facilitate intracellular drug delivery. In this article, the mechanism of PCI technology and its development for gene delivery were reviewed, which can provide the scientific basis for the possible utilization of PCI to solve the problem of endo-lysosomal escape in gene delivery.

Key words: gene delivery, photochemical internalization (PCI), photosensitizer, reactive oxygen species, endo-lysosomal escape