Whole gene expression profile analysis of miRNAs in human umbilical vein endothelial cells regulated by vascular endothelial growth factor A

doi:10.3969/j.issn.1674-8115.2021.09.008

Abstract

Abstract: Objective

·To analyze the changes of microRNA (miRNA) in human umbilical vein endothelial cells (HUVECs) under the stimulation of vascular endothelial growth factor A (VEGFA), in order to further explore the roles of miRNA in regulating the expression of downstream genes of VEGFA, and to find new regulatory miRNAs for angiogenesis.

Methods

·The miRNA expression profile was analyzed by using NanoString nCounter in HUVECs 0, 1, 4, and 12 h after VEGFA stimulation. The differentially expressed miRNAs with the same trend were clustered and their target genes were predicted. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to analyze and predict the functions of these target genes, and the interaction networks were established.

Results

·The expression of 39 miRNAs changed significantly after VEGFA stimulation in HUVECs. Bioinformatic analysis showed that there were 129 target mRNAs corresponding to the differentially expressed miRNAs which were involved in multiple biological processes and signal pathways related to angiogenesis.

Conclusion

·miRNAs are differentially expressed in HUVECs after stimulation by VEGFA, and miRNAs play important roles in regulating angiogenesis. MiR-107 and miR-21 may severe as candidate functional regulators of angiogenesis.

Key words: microRNA (miRNA), vascular endothelial growth factor A (VEGFA), human umbilical vein endothelial cell (HUVEC), bioinformatic analysis

CLC Number:

Li LIU, Zi-long GENG, Jia-huan CHEN, Sha-sha ZHANG, Bing ZHANG. Whole gene expression profile analysis of miRNAs in human umbilical vein endothelial cells regulated by vascular endothelial growth factor A[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(9): 1183-1189.

Figures/Tables 5

Fig 1 Target genes of miDEGs

Fig 2 Heat map of differentially expressed miRNAs (miDEGs)

Fig 3 GO function analysis of target genes of miDEGs

Fig 4 KEGG pathway analysis of target genes of miDEGs

Fig 5 Network of miDEGs and their target genes

TrendMD

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