Abstract:

Objective To evaluate the strengthening and restoring effects and hepatoprotective effect of Eclipta extracts. Methods Sixty Kunming mice were randomly divided into control group, low-dose Eclipta group, high-dose Eclipta group, cyclophosphamide(CY) group, low-dose Eclipta CY group and high-dose Eclipta CY group (n=10). Eclipta 8 g·kg^-1·d^-1 or 4 g·kg^-1·d^-1 were administered by oral gavage for 7 d. Immunosuppression mouse models were established by intraperitoneal injection of 0.05 g/kg CY on the fourth day of administration. The effects of Eclipta extracts on thymus index, spleen index and carbon clearance index in both normal mice and immunosuppression mice induced by CY were tested, and those on proliferation of lymphocyte and T cells induced by ConA were examined. Based on these data, strengthening and restoring effects of Eclipta were observed. Besides, 70 Kunming mice were randomly divided into control group, low-dose Eclipta group, high-dose Eclipta group, CCl₄ group, DDB group, low-dose Eclipta CCl₄ group and high-dose Eclipta CCl₄ group. 20 g·kg^-1·d^-1 and 10 g·kg^-1·d^-1 Eclipta were administered by oral gavage for 7 d. Hepatic injury mouse models were established by intraperitoneal injection of 10 mL/kg CCl₄ 2 h after the last administration. The effects of Eclipta extracts on serum ALT, MDA, TP and T-Bil levels in both normal mice and hepatic injury mice induced by CCl₄ was tested, and those on the viability of the primary culture hepatocytes were determined. Based on these data, hepatoprotective effect of Eclipta was observed. Results Eclipta extracts increased thymus index and carbon clearance index in both normal mice and immunosuppression mice induced by CY, promoted proliferation of lymphocyte and proliferation of T cells induced by ConA, reduced serum ALT and MDA levels in hepatic injury mice, and improved the viability of the primary culture hepatocytes. Conclusion Eclipta herb has strengthening and restoring effects and hepatoprotective effect.

Key words: Eclipta L., cyclophosphamide, CCl₄, strengthening and restoring effects, hepatoprotective effect