›› 2010, Vol. 30 ›› Issue (12): 1513-.doi: 10.3969/j.issn.1674-8115.2010.12.016

• Original article (Basic research) • Previous Articles     Next Articles

Effects of benzo(a)pyrene on expression of cytochrome P450 1A1 in HeLa cells of uterine cervix cancer

SHI Yu-rong1,2, GENG Jian1,2, WANG Hui2, ZHANG Yao3   

  1. 1.Department of Biochemistry and Molecular Biology, 2.Research Center of Clinical Laboratory Science, Bengbu Medical College, Bengbu 233000, China；3.Department of Biochemistry, Wannan Medical College, Wuhu 241002, China
  • Online:2010-12-25 Published:2010-12-31
  • Supported by:

    Research Foundation for Young Teachers in Colleges of Anhui Province, 2008jq1107

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Abstract:

Objective To investigate the effects of benzo(a)pyrene (BaP) on the proliferation of HeLa cells of uterine cervix cancer and expression of cytochrome P450 1A1 (CYP1A1) in HeLa cells of uterine cervix cancer. Methods HeLa cells of uterine cervix cancer were treated with different concentrations of BaP (0, 1.0, 2.5, 5.0, 7.5 and 10.0 μmol/L). The effect of BaP on the proliferation of HeLa cells was detected by MTT assay, the expression of CYP1A1 protein and mRNA was determined by Western blotting and RT-PCR, respectively, and the activity of CYP1A1 was measured by fluorescein labeling. Results MTT assay revealed that optical density (D553 nm) of groups of different concentrations of Bap was higher than that of control group (0 μmol/L BaP), with the highest value in 7.5 μmol/L BaP group (P<0.05). RT-PCR and Western blotting indicated that the expression of CYP1A1 mRNA and protein of groups of different concentrations of Bap was higher than that in control group, with the highest value in 7.5 μmol/L BaP group (P<0.05). The activity of CYP1A1 in groups of different concentrations of BaP was higher than that in control group, with the highest value in 5.0 μmol/L BaP group (P<0.05). Conclusion BaP can promote the proliferation of HeLa cells, and increase the expression and activity of CYP1A1 in HeLa cells.

Key words: benzo(a)pyrene, cytochrome P450 1A1, HeLa cells