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    Editorial
    Laser and tissue wound repair
    YAO Min, FANG Yong, WANG Ying
    2010, 30 (12):  1451. 
    doi: 10.3969/j.issn.1674-8115.2010.12.001

    Abstract ( 1761 )   PDF (4096KB) ( 1295 )  

    Laser has been widely used in medical area. Low level laser therapy can promote cell growth through biostimulation. Laser may initiate photochemical reaction that causes light-induced polymerization, which can assist in various tissue wound closures. In addition, laser can be utilized to treat wound infection efficiently by photooxidation reaction. An effective improvement of scar reduction can be produced by fractional laser treatment. Laser may play more important roles in tissue wound repair in the future.

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    Monographic report (Traumatic medicine)
    Construction of mouse pLVX-Wnt3a-IRES-ZsGreen1 lentivirus vectors and transfection of neural stem cells
    BI Yong-yan, PAN Dong-chao, FENG Dong-fu, et al
    2010, 30 (12):  1455. 
    doi: 10.3969/j.issn.1674-8115.2010.12.002

    Abstract ( 2627 )   PDF (7073KB) ( 1871 )  

    Objective To construct lentivirus vectors with co-expression of mouse Wnt3a(mWnt3a) gene and green fluorescent protein (GFP), transfect into neural stem cells (NSCs), and observe the expression of mWnt3a in NSCs. Methods Gene recombinant technology was employed to clone mWnt3a gene to lentivirus vector pLVX-IRES-ZsGreen1 to construct a lentiviral vector pLVX-Wnt3a-IRES-ZsGreen1. Lentiviral vectors were packaged and the titer was determined. NSCs were transfected with the constructed pLVX-Wnt3a-IRES-ZsGreen1 (Wnt3a-NSCs group) or pLVX-IRES-ZsGreen1 (GFP-NSCs group), with normal NSCs (NSCs group) as controls. NSCs of Wnt3a-NSCs group were identified by immunoflourence staining for nestin, the expression of mWnt3a mRNA in NSCs of each group was detected by Real-Time PCR, and Western blotting was applied to detect the expression of mWnt3a and β-catenin protein in NSCs of each group. Results It was confirmed by restriction enzyme digestion, DNA sequencing analysis and GFP expression detection that recombinant pLVX-Wnt3a-IRES-ZsGreen1 vector was successfully constructed, with virus titer up to 3×108 TU/mL. Not only green fluorescence but also expression of nestin were observed in NSCs of Wnt3a-NSCs group. Real-Time PCR and Western blotting revealed that the expression of mWnt3a mRNA and protein and β-catenin protein in Wnt3a-NSCs group was significantly higher than that in GFP-NSCs group and NSCs group 7 d after transfection (P<0.01). Conclusion The recombinant lentiviral vector carrying mWnt3a can be successfully constructed, and mWnt3a gene can be successfully transfected into NSCs in vitro.

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    Effects of hypertonic sodium chloride hydroxyethyl starch 40 on cerebral perfusion of pigs with traumatic brain injury and hemorrhagic shock
    LUO Wei, LI Xue-yuan, SHEN Bo-xiong, et al
    2010, 30 (12):  1460. 
    doi: 10.3969/j.issn.1674-8115.2010.12.003

    Abstract ( 1650 )   PDF (6782KB) ( 1597 )  

    Objective To investigate the effects of hypertonic sodium chloride hydroxyethyl starch 40 (HSH40) on cerebral perfusion of pigs with traumatic brain injury (TBI) and hemorrhagic shock. Methods Models of TBI, acute intracranial hypertension and hemorrhagic shock were established in 12 miniature pigs under anesthesia and mechanical ventilation by controlled cortical impact, epidural balloon method and blood withdrawal via femoral artery. One hour after model establishment, animals were divided into three groups (n=4), and were intravenously infused with hydroxyethyl starch 200/0.5 (HES group, equivalent volume of removed blood), hypertonic saline 7.5% (HS group, 20% volume of removed blood) and HSH40 (HSH group, 35% volume of removed blood), respectively. Physiological data, including mean arterial pressure(MAP), intracranial pressure(ICP) and cerebral perfusion pressure (CPP) were observed before model establishment(T01), 60 min after model establishment (before resuscitation)(T0) and 15 min(T15), 30 min(T30), 60 min(T60), 120 min(T120) and 180 min(T180) after resuscitation. Besides, changes of serum sodium, plasma osmotic pressure(OSM), arteriovenous oxygen difference (Da-jvO2) and cerebral oxygen enhancement ratios (OER) of T01, T0, T15, T60 and T120 were observed. Results After resuscitation treatment, MAP in each group significantly increased (P<0.05), and the increase in HSH group was the fastest. After reaching the peak, MAP in HSH group and HES group slowly decreased, while MAP in HS group decreased fast. After resuscitation treatment, ICP in HSH group and HS group of each time point decreased significantly (P<0.05), whereas an elevated ICP occurred in HES group (P<0.05 except for T15). After resuscitation treatment, CPP in each group increased fast, and decreased after reaching the peak, with CCP of each time point significantly higher than those of T0 (P<0.05). CPP in HSH group and HS group increased faster than that in HES group, with HSH group higher than HES group at each time point after resuscitation treatment (P<0.05) and higher than HS group after reaching the peak (P<0.05). Da-jvO2 and OER of T0 in each group were significantly higher than those of T01 (P<0.05), and Da-jvO2 and OER of time points after resuscitation treatment were significantly lower than those of T0 (P<0.05). OER in HS group and HSH group of T60 decreased to those of T01 (P>0.05), while OER of T120 was significantly higher than that of T01 in HS group (P<0.05). Serum sodium and OSM of each time point after resuscitation treatment were significantly higher than those of T0 in HSH group and HS group (P<0.05), and the peak values reached at T15. Conclusion HSH40 (6.6 mL/kg) can effectively resuscitate MAP, reduce ICP, increase CCP and cerebral blood flow, and improve the balance of supply and demand of cerebral oxygen of pigs with TBI and hemorrhagic shock.

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    Logistic regression analysis of influential factors for prognosis of diffuse axonal injury
    WANG Hong-cai, CHEN Hai, WANG Bo-ding, et al
    2010, 30 (12):  1466. 
    doi: 10.3969/j.issn.1674-8115.2010.12.004

    Abstract ( 1966 )   PDF (4267KB) ( 1305 )  

    Objective To explore the influential factors for long-term prognosis of diffuse axonal injury (DAI). Methods Prospective cohort study was performed on 75 patients with DAI, and related data of DAI and follow-up findings 6 months after injury were recorded. Single and multiple factor analysis were carried out to analyze the relationship between related factors and prognosis of DAI. Logistic regression analysis was employed for multiple factor analysis. Results Logistic regression analysis revealed that Glasgow coma score (GCS) score on admission (OR=0.021, 95% CI 0.007-1.557), bilateral pupillary abnormality (OR=27.532, 95% CI 8.975-57.413), number of lesions of brain central areas on diffusion weighted imaging of MRI (OR=5.753, 95% CI 2.136-9.392) and average apparent diffusion coefficient of brain central areas (OR=0.353, 95% CI 0.134-0.964) were influential factors for prognosis of DAI. The combination of the above factors provided a predictive accuracy of 92.0% for prognosis of DAI. Conclusion GCS on admission, bilateral pupillary abnormality, number of lesions of brain central areas on diffusion weighted imaging of MRI and average apparent diffusion coefficient of brain central areas are independent factors for prognosis of DAI. Accurate prognosis prediction for DAI may be provided on the basis of clinical and imaging findings.

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    Acellular amniotic membrane integrated with hair follicle stem cells in repair of full-thichness skin defects in nude mice
    HU Xiao-hui, YANG Peng-gao, GAO Feng-hou, et al
    2010, 30 (12):  1470. 
    doi: 10.3969/j.issn.1674-8115.2010.12.005

    Abstract ( 1695 )   PDF (5410KB) ( 1523 )  

    Objective To repair full-thichness skin defects by acellular amniotic membrane integrated with hair follicle stem cells. Methods Human hair follicle stem cells were isolated and cultured in vitro, and those of the fourth generation marked by lentivirus-mediated green fluorescent protein(pGC FU-GFP-Lentiviru) were grafted onto acellular amniotic membrane. Seven days after integration, cell proliferation was observed by light microscopy with HE staining. Wounds of full-thickness skin defects were prepared in 18 C57BL/6 nude mice, and experiment group (wounds of fullthickness skin defects were grafted with acellular amniotic membrane integrated with hair follicle stem cells), acellular amniotic membrane graft+stem cell injection group (wounds of full-thickness skin defects were grafted with acellular amniotic membrane, with injection of 5×106 hair follicle stem cells under amniotic membrane) and acellular amniotic membrane graft group (wounds of full-thickness skin defects were grafted with acellular amniotic membrane) were divided, with 6 mice in each group. The areas and contraction rates of wounds in each group were measured 7 d, 14 d, 21 d and 28 d after grafting. The expression of green fluorescent protein (GFP) of wound tissues was detected by fluorescence microscopy, and the structure of regenerated skins was observed by light microscopy with HE staining. Results Seven days after acellular amniotic membrane integration with hair follicle stem cells, light microscopic observation with HE staining revealed that cells formed in lamellar shape and covered acellular amniotic membrane. The contraction rates of wounds of each time points in experiment group and acellular amniotic membrane graft+stem cell injection group were significantly lower than that in acellular amniotic membrane graft group (P<0.05). Twentyeight days after grafting, fluorescence microscopic observations revealed that the expression of GFP in cuticular layer of wounds in experiment group was positive, and light microscopic observations with HE staining unveiled that the cuticular layer of wounds in experiment group was significantly thickened, and hair follicle-like structure formed. Conclusion Acellular human amniotic membrane integrated with human hair follicle stem cells can be used in the repair of full-thichness skin defects in nude mice.

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    Protective effect of ulinastatin on lung tissues in early stage of serious burn
    XU Peng, FANG Yong, YU Wei-rong, et al
    2010, 30 (12):  1474. 
    doi: 10.3969/j.issn.1674-8115.2010.12.006

    Abstract ( 1572 )   PDF (3084KB) ( 1299 )  

    Objective To investigate the effect of conventional fluid resuscitation combined with ulinastatin treatment on acute lung tissue injury (ALI) in early stage of serious burn. Methods The clinical data of patients with area of burn ≥50% total body surface area (TBSA) or area of burn of III degree ≥20% TBSA (patients with inhalation injury were excluded) were collected and retrospectively analysed. Among the 69 patients, 36 received conventional fluid resuscitation treatment (control group), and the other 33 were managed with conventional fluid resuscitation combined with ulinastatin (UTi group). ALI scoring was performed on the basis of ratio of arterial partial pressure of oxygen (PaO2) to fraction of inspired oxygen (PaO2/FiO2), respiratory frequency and chest X-ray findings before treatment and 1 d, 3 d and 5 d after treatment, and ALI scores and prevalences of ALI were compared between two groups. Results There was no significant difference in ALI scores before treatment and 1 d after treatment between two groups (P>0.05), while ALI scores in UTi group were significantly lower than those in control group 3 d and 5 d after treatment (P<0.05). The prevalence of ALI in UTi group was significantly lower than that in control group (45.6% vs 63.9%, P<0.05). Conclusion Conventional fluid resuscitation combined with ulinastatin treatment decreases the prevalence of ALI in early stage of serious burn, which has protective effect on lung tissues.

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    Effects of adaptive support ventilation plus lung recruitment maneuvers on acute respiratory distress syndrome
    QIN Zong-he, YING You-guo, MAO Yong
    2010, 30 (12):  1477. 
    doi: 10.3969/j.issn.1674-8115.2010.12.007

    Abstract ( 2056 )   PDF (4021KB) ( 1273 )  

    Objective To investigate the effects of adaptive support ventilation (ASV) plus lung recruitment maneuvers (LRM) on acute respiratory distress syndrome (ARDS). Methods Twenty-eight patients with ARDS after trauma were collected, and were randomly treated with ASV or intermittent positive pressure ventilation (IPPV) after management with IPPV for 8 h. The minute ventilation in ASV was the same with IPPV. Positive end expiratory pressure (PEEP) level increased in each model by 0, 5 and 10 cmH2O, and each level of PEEP was maintained for 60 min. LRM was added to each PEEP level in ASV model (ASV+LRM model). Three hours after the previous ventilation model, it was adjusted to IPPV with 0 cmH2O PEEP, then to the other model 1 h later. Hemodynamics, respiratory mechanics and oxygen metabolism parameters were measured with Swan-Ganz catheter, electrocardiogram monitor and breathing machine after patients were ventilated with each level of PEEP for 50 min. Results Under the same PEEP level, there was no significant difference in hemodynamic parameters between ASV+LRM model and IPPV model (P>0.05), while the peak inspiratory pressure in ASV+LRM model was significantly lower than that in IPPV model (P<0.05), and lung dynamic compliance, partial pressure of oxygen in artery and oxygen delivery in ASV+LRM model were significantly higher than those in IPPV model with PEEP=5 cmH2O (P<0.05). Conclusion ASV+LPS model is better than IPPV in ventilation for patients with ARDS.

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    Oxidative injury and antioxidant therapy in early stage of burn
    FU Xiu-jun, FANG Yong
    2010, 30 (12):  1481. 
    doi: 10.3969/j.issn.1674-8115.2010.12.008

    Abstract ( 1522 )   PDF (5584KB) ( 1628 )  

    In the early stage of severe burn, ischemia-reperfusion injury caused by fluid loss and fluid resuscitation and changes in nerve and body fluid caused by stress may lead to severe oxidative damage, which is one of the important mechanisms of multiple organ injury. The research progress of oxidative injury from reactive oxygen species and antioxidant therapy in the early stage of severe burn is reviewed in this paper.

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    Effects of emergency surgical internal fixation management on hemodynamics in patients with traumatic flail chest
    TENG Ji-ping, NI Da, CHENG You-shuang, et al
    2010, 30 (12):  1486. 
    doi: 10.3969/j.issn.1674-8115.2010.12.009

    Abstract ( 1750 )   PDF (3929KB) ( 1442 )  

    Objective To investigate the effects of emergency surgical internal fixation on hemodynamics in patients with traumatic flail chest. Methods A total of 34 patients with traumatic flail chest managed by emergency surgical internal fixation were selected. The parameters of heart rate (HR), cardiac output (CO), pulmonary capillary wedge pressure (PCWP), mean arterial pressure (MAP) and central venous pressure (CVP) at the time points of before anesthesia induction, before operation after anesthesia, immediately after operation, 4 h after operation and 24 h after operation were monitored and compared. Results Compared with those at the time points of before anesthesia induction and before operation after anesthesia, HR and CVP significantly decreased (P<0.05), and CO, PCWP and MAP significantly increased at the time points of immediately after operation, 4 h after operation and 24 h after operation (P<0.05). Conclusion Emergency surgical internal fixation may improve the hemodynamics of traumatic flail chest, and has some short-term therapeutic effect on flail chest.

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    Establishment of rabbit model of cicatricial laryngotracheal stenosis
    YANG Xi-zhi, MAO Xiao-hui, AO Hua-fei, et al
    2010, 30 (12):  1490. 
    doi: 10.3969/j.issn.1674-8115.2010.12.010

    Abstract ( 2212 )   PDF (5921KB) ( 1417 )  

    Objective To establish a simple and stable rabbit model of cicatricial laryngotracheal stenosis. Methods Twenty New Zealand rabbits were divided into experiment group (n=10) and control group (n=10).  Tracheas of rabbits in experiment group were annularly incised, and were closed after scraping of tracheal mucosa with a nylon brush. Tracheas of rabbits in control group were closed immediately after incision, without any special treatment. Formation of cicatrix of trachea was observed by endoscopy on day 4, 9, 14 and 19, and the degree of stenosis was calculated. Rabbits were sacrificed three weeks later, laryngopharyngeal samples were obtained, and microscopic observations were conducted with HE staining. Results Endoscopic observations revealed that there was no cicatricial tissue hyperplasia in rabbits of control group. However, cicatricial tissue hyperplasia occurred and increased in rabbits in experiment group in a time-dependent manner, and the degree of tracheal stenosis 19 d after scraping was more severe than that 4 d after scraping (P<0.05). Proliferation of fibroblasts and thickening of collagen fibers in areas of tracheal stenosis were observed by microscopic observations. Conclusion The rabbit model of cicatricial laryngotracheal stenosis can be easily established, which can serve in the research of treatment of laryngotracheal stenosis.

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    One case report of bilateral occipital lobe infarction combined with Anton syndrome after traumatic cerebral herniation
    CHEN Er-tao, YANG Xi-tao, CHU Sheng-hua, et al
    2010, 30 (12):  1494. 
    doi: 10.3969/j.issn.1674-8115.2010.12.011

    Abstract ( 1750 )   PDF (2160KB) ( 1082 )  

    This paper reported a case of bilateral occipital lobe infarction combined with Anton syndrome after traumatic cerebral herniation. The patient did not receive early postoperative visual function examinations due to no complaint of visual dysfunction. Three weeks after operation, fixed eyeballs and upward gaze were found during physical examination, and bilateral occipital lobe infarction was revealed by CT and MRI. The patient was then managed with dehydration, neurotrophy and hyperbaric oxygen treatment, while the effect was limited. On basis of literature review, some issues related to this case were discussed.

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    Original article (Basic research)
    Mouse gastric emptying assessment by domestic 13C-octanoic acid breath test system
    ZHANG Min, GUAN Liang, LI Biao, et al
    2010, 30 (12):  1496. 
    doi: 10.3969/j.issn.1674-8115.2010.12.012

    Abstract ( 1995 )   PDF (5974KB) ( 1435 )  

    Objective To establish a domestic 13C-octanoic acid breath test system for gastric emptying assessment, evaluate the abnomal gastric emptying and investigate the effect of gastroprokinetic agents on gastric empting in diabetic mice. Methods 13C-octanoic acid was synthesized with Grignard method. Diabetic mouse models were established by intravenous injection of alloxan tetrahydrate. After gastric irrigation with 13C-octanoic acid to mice, expired air samples were collected at various time points (0, 5, 10, 15, 20, 25, 30, 45, 60, 75, 90, 105 and 120 min)and were examined with an infra red isotope analyzer for maximum concentration (Cmax) and half emptying time (T1/2) of 13CO2. Effect of 13C-octanoic acid dosage on gastric emptying parameters and test reproducibility were analyzed. The effect of gastroprokinetic agent intervention on gastric empting of diabetic mice was evaluated, and the abnormal toxicity of 13C-octanoic acid was observed. Results 13C-octanoic acid dosage was positively related to Cmax (r=0.954,P<0.05), while had no significant effect on T1/2. The coefficient of variation for reproducibility was (4.76±1.43)%. There was no significant difference in T1/2 between normal group and diabetes group [(28.2±6.8) min vs (28.1±4.8) min, P>0.05]. Ghrelin significantly decreased T1/2 to (19.6±4.9) min (P<0.05), while atropine significantly increased T1/2 to (40.7±7.0) min (P<0.05) in diabetic mice. No apparent toxicity was observed in 13C-octanoic acid abnormal toxicity test. Conclusion The established 13C-octanoic acid breath test system can evaluate the gastric emptying and determine the effect of gastroprokinetic agents on gastric empting in mice, with no invasion and favourable reproducibly.

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    Construction of mouse osteoinductive factor gene retroviral vector and its expression in 293T cells
    ZHENG Cui-xia, ZHANG Xiao-na, HAN Bing, et al
    2010, 30 (12):  1501. 
    doi: 10.3969/j.issn.1674-8115.2010.12.013

    Abstract ( 2074 )   PDF (5927KB) ( 1455 )  

    Objective To construct mouse osteoinductive factor (OIF) gene recombinant retroviral vector, and investigate its expression in 293T cells. Methods OIF-3FLAG gene was amplified with PCR and cloned into retroviral vector (Puro-IRES-GFP) after sequencing, and recombinant retroviral vector pMSCV PIG-OIF-3FLAG was constructed and identified by sequencing. PMSCV PIG-OIF-3FLAG retroviral vector with helper vectors of VSAG and GAG-POL were co-transfected into 293T cells by lipofectamine2000. Forty-eight hours after transfection, the expression of green fluorescent protein (GFP) was examined by fluorescence microscopy. The supernatant of 293T cells, which were tansfected with pMSCV PIG-OIF-3FLAG retroviral vector and helper vectors of VSAG and GAG-POL, was used to reinfect 293T cells. After 48 h, 293T cells stably expressing pMSCV PIG-OIF-3FLAG retroviral vector were screened by 3 μg/mL puromycin for 7 d, and the expression of OIF mRNA and protein of 293T cells was detected by Real-Time PCR and Western blotting, respectively. Results OIF gene retroviral vector pMSCV PIG-OIF-3FLAG was successfully constructed, and the cloning site and reading frame of objective gene were confirmed by enzyme digestion and sequencing. Then, 293T cells were infected by retrovirus supernatant, and 293T cells with stable expression of mouse OIF were obtained by puromycin screening. Real-Time PCR and Western blotting revealed high expression of OIF mRNA and protein in 293T cells. Conclusion Mouse OIF gene retroviral vector has been successfully constructed, and 293T cells with stable expression of OIF gene are obtained.

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    Changes of islet function of rats with lower birth weight and catch-up growth
    TIAN Jing-yan, WANG Wei-qing, LI Feng-ying, et al
    2010, 30 (12):  1505. 
    doi: 10.3969/j.issn.1674-8115.2010.12.014

    Abstract ( 1313 )   PDF (4635KB) ( 1958 )  

    Objective To investigate the effects of lower birth weight and catch-up growth on islet function of rats. Methods An animal model of lower birth weight (birth weight lower than 2 standard deviations of the average of control) and high fat diet was established (model group), and the other animal model of normal diet after normal birth was used as control (normal group). The body weight of rats aged 3, 7, 11 and 15 weeks and the weight of epididymal fat were recorded and compared in two groups. Glucose metabolism was evaluated by oral glucose tolerance test (OGTT), and insulin sensitivity was determined by hyperinsulinaemic-euglycaemic clamp test in two groups of rats aged 15 weeks. Double-label immunofluorescence assay was adopted to observe the islet architecture of two groups of rats aged 16 weeks. Results The body weight and weight of epididymal fat of rats aged 15 weeks in model group were significantly higher than those in normal group (P<0.05). Fasting blood glucose and blood glucose of 30, 60 and 120 min after glucose loading in model group were significantly higher than those in normal group (P<0.05), the steady-state blood glucose and basal insulin in model group were significantly higher than those in normal group (P<0.05), while the glucose infusion rate in model group was significantly lower than that in normal group (P<0.05). Double-label immunofluorescence assay revealed that the islet architecture of rats in model group was disrupted, and the ratio of area of insulin to total area of islet in model group (0.59±0.09) was significantly lower than that in normal group (0.73±0.08)(P<0.05). Conclusion Rats with lower birth weight and catch-up growth have impaired glucose tolerance and insulin resistance, which may be associated with the reconstruction of islet structure and reduction of β cell mass.

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    Construction and self-activation of DLX4 in yeast two-hybrid system
    QIAO Qin-qin, SUN Yun-yan, CHEN Ling-ling, et al
    2010, 30 (12):  1509. 
    doi: 10.3969/j.issn.1674-8115.2010.12.015

    Abstract ( 2050 )   PDF (4986KB) ( 1562 )  

    Objective To construct a yeast-two hybrid system of DLX4 and identify its self-activation. Methods Human placenta yeast-two cDNA bank was constructed, and was identified by PCR. DLX4 bait plasmids including pDEST32-DLX4(4-1C), pDEST32-DLX4(4-1N) and pDEST32-DLX4(4-2) were constructed and transfected into yeast MaV203 cells, and the self-activation effect was detected. Results It was verified by PCR that human placental cDNA bank was successfully constructed. pDEST32-DLX4(4-2) and pDEST32-DLX4(4-1C) had no self-activation effect, and could be used in the yeast two-hybrid system. Conclusion Yeast-two hybrid bank and DLX4 bait plasmids have been successfully constructed, and can be used to screen the protein interacting with DLX4.

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    Effects of benzo(a)pyrene on expression of cytochrome P450 1A1 in HeLa cells of uterine cervix cancer
    SHI Yu-rong, GENG Jian, WANG Hui, et al
    2010, 30 (12):  1513. 
    doi: 10.3969/j.issn.1674-8115.2010.12.016

    Abstract ( 1586 )   PDF (4475KB) ( 1362 )  

    Objective To investigate the effects of benzo(a)pyrene (BaP) on the proliferation of HeLa cells of uterine cervix cancer and expression of cytochrome P450 1A1 (CYP1A1) in HeLa cells of uterine cervix cancer. Methods HeLa cells of uterine cervix cancer were treated with different concentrations of BaP (0, 1.0, 2.5, 5.0, 7.5 and 10.0 μmol/L). The effect of BaP on the proliferation of HeLa cells was detected by MTT assay, the expression of CYP1A1 protein and mRNA was determined by Western blotting and RT-PCR, respectively, and the activity of CYP1A1 was measured by fluorescein labeling. Results MTT assay revealed that optical density (D553 nm) of groups of different concentrations of Bap was higher than that of control group (0 μmol/L BaP), with the highest value in 7.5 μmol/L BaP group (P<0.05). RT-PCR and Western blotting indicated that the expression of CYP1A1 mRNA and protein of groups of different concentrations of Bap was higher than that in control group, with the highest value in 7.5 μmol/L BaP group (P<0.05). The activity of CYP1A1 in groups of different concentrations of BaP was higher than that in control group, with the highest value in 5.0 μmol/L BaP group (P<0.05). Conclusion BaP can promote the proliferation of HeLa cells, and increase the expression and activity of CYP1A1 in HeLa cells.

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    Effects of GnRH agonist on cell cycle and expression of anti-apoptosis protein cFLIP of ovarian granulosa cells in patients with endometriosis
    LU Xiang, LI Lu, WU Yu, et al
    2010, 30 (12):  1517. 
    doi: 10.3969/j.issn.1674-8115.2010.12.017

    Abstract ( 1826 )   PDF (5658KB) ( 1397 )  

    Objective To investigate the effects of GnRH agonist (GnRH-a) on cell cycle and expression of anti-apoptosis protein cFLIP of ovarian granulosa cells in patients with endometriosis. Methods Forty-five women treated with in vitro fertilization and embryo transfer (IVF-ET) were selected, among whom 19 were patients with endometriosis and randomly assigned to GnRH-a treatment group (group A, n=11) and general long protocol group (group B, n=8). The other 26 women who were infertile due to tubal factors and treated with general long protocol were served as control group(group C). Granulosa cells were obtained from follicular fluid, cell cycle was analyzed by flow cytometry, and the expression of cFLIP protein in granulosa cells was detected by Western blotting. Results There was no significant difference in fertilization rates, clinical pregnancy rates, implantation rates and miscarriage rates among three groups (P>0.05). There was no significant difference in the percentage of G0/G1 phase cells among three groups (P>0.05). The percentage of S phase cells was significantly higher, that of G2/M phase cells was significantly lower, and that of apoptosis peak was significantly higher in group A and group B than those in group C (P<0.05). However, there was no significant difference in cell cycle between group A and goup B (P>0.05). The expression of cFLIP protein in ovarian granulosa cells in group C was significantly higher than that in group A and group B (P<0.05), while there was no significant difference between group A and group B (P>0.05). Conclusion The apoptosis of ovarian granulosa cells in patients with endometriosis increases, with abnormal cell cycle. Treatment with prolonged GnRH-a protocol may not improve the abnormal status of impaired cell cycle and lower expression of cFLIP.

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    Original article (Clinical research)
    Oxidative stress in patients with chronic kidney diseases of different clinical stages
    WANG Wen-ji, ZHANG Wei
    2010, 30 (12):  1521. 
    doi: 10.3969/j.issn.1674-8115.2010.12.018

    Abstract ( 1943 )   PDF (3994KB) ( 1389 )  

    Objective To observe and compare the oxidative stress in patients with chronic kidney diseases (CKD) of different clinical stages. Methods One hundred and sixty-three patients with CKD were enrolled, and CKD1 to CKD5 groups were divided according to estimated glomerular filtration rate (eGFR). Besides, patients in CKD5 group were subdivided into CKD5-hemodialysis group and CKD5-non-hemodialysis group according to dialysis management. Serum malonaldehyde (MDA) level and superoxide dismutase (SOD) activity were detected in each group, and statistical analysis was conducted. Results Serum MDA level and SOD activity had linear correlation with eGFR (R2=0.547,R2=0.406;P<0.05). For CKD2 group, CKD3 group and CKD4 group, serum MDA level was highest in CKD4 group and lowest in CKD2 group (P<0.05), and SOD activity was lowest in CKD4 group and highest in CKD2 group (P<0.05). However, there was no significant difference in these two parameters between CKD4 group and CKD5 group and between CKD2 group and CKD1 group (P>0.05). There were significant differences in serum MDA levels and SOD activity between CKD5-hemodialysis group and CKD5-non-hemodialysis group [(9.20±1.75)nmol/mL vs (7.01±1.40) nmol/mL and (59.24±17.82)U/mL vs (72.21±17.82)U/mL, respectively (P<0.05)]. Conclusion Oxidative stress increases with renal function decrease in patients with CKD. Compared with CKD5 patients without hemodialysis, those managed with hemodialysis have more severe oxidative stress.

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    Effect of risperidone on sensory gating P50 deficit in patients with schizophrenia
    LIU Deng-tang, ZHUO Kai-ming, SONG Zhen-hua, et al
    2010, 30 (12):  1525. 
    doi: 10.3969/j.issn.1674-8115.2010.12.019

    Abstract ( 1752 )   PDF (5354KB) ( 1632 )  

    Objective To investigate the effect of atypical antipsychotic medicine risperidone on sensory gating P50 deficit in patients with schizophrenia. Methods Twenty-six patients with first-episode schizophrenia and 25 patients with chronic schizophrenia were enrolled in the study. All patients were treated with risperidone of different doses (2 to 6 mg/d). All patients fulfilled the evaluation of sensory gating P50 with the conditioning (S1)-testing (S2) paradigm before treatment and 4 to 6 weeks after treatment. The psychotic symptoms were assessed with Positive and Negative Syndrome Scale (PANSS), and the therapeutic effects were evaluated with PANSS reduction rate. Results There was no significant difference in P50 parameters between the two groups before treatment (P>0.05), and there was no significant correlation between P50 inhibition parameters and disease course, psychotic episodes and psychiatric symptoms (PANSS total score, positive symptoms score, negative symptoms score and general psychopathology symptoms score) of schizophrenia (P>0.05). Except the group main effect for S2 amplitude (P=0.02), there was no significant change for main effect and interaction of the other P50 parameters after treatment (P>0.05). The effect of risperidone on P50 parameters was not related to the therapeutic effect. Conclusion Deficit in sensory gating inhibition exists in both first-episode schizophrenia and chronic schizophrenia, and risperidone in not effective in treating the deficit in sensory gating inhibition of schizophrenia.

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    Effects of serum of patients with maintenance hemodialysis and dialysates on apoptosis of adipocytes
    CAI Ling-ling, GUO Li-li, ZHU Xiao-juan, et al
    2010, 30 (12):  1530. 
    doi: 10.3969/j.issn.1674-8115.2010.12.020

    Abstract ( 1856 )   PDF (7067KB) ( 1259 )  

    Objective To investigate the effects of serum of patients with maintenance hemodialysis (HD), conventional dialysate (CD) and high purity dialysate (HPD) on apoptosis of adipocytes. Methods Blood samples of 20 patients with HD (ten were treated with CD and ten were treated with HPD) were collected, and serum was isolated for detection of tumor necrosis factor-α (TNF-α) by ELISA. Mature 3T3-L1 adipocytes induced in vitro were treated with hemodialysis patient serum (HDPS group), HDPS combined with CD (HDPS+CD group) and HDPS combined with HPD (HDPS+HPD group), respectively, and cells were harvested 48 h later. Morphological changes were observed by Hoechst33258 fluorescence staining, and cell apoptosis was detected by Annexin-V-FITC/PI double staining. Bacterial DNA from CD and HPD was assayed by PCR. Results It was revealed by ELISA that the serum level of TNF-α from patients with HD treated by CD was significantly higher than that from patients with HD treated by HPD (P<0.05). Typical apoptotic morphology changes were observed by fluorescence microscopy in HDPS group and HDPS+CD group, and the apoptosis rates in these two groups were significantly higher than that in HDPS+HPD group (P<0.05). PCR revealed that there were significant differences between the expression of bacterial DNA in CD and that in HPD (902.79±60.57 vs 454.87±32.22)(P<0.05). Conclusion HDPS may induce apoptosis of 3T3-L1 adipocytes, which may be related to the increased serum level of TNF-α. Compared with intervention by HDPS and CD, intervention by HDPS and HPD may yield lower apoptosis rate of adipocytes, and there may be lower expression of bacterial DNA in HPD.

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    Efficacy of hemicellulose dressing for wound healing of donor sites of burn or reconstruction
    LIU Jian, ZHENG Jie-xin, SHI Yan, et al
    2010, 30 (12):  1535. 
    doi: 10.3969/j.issn.1674-8115.2010.12.021

    Abstract ( 1779 )   PDF (5279KB) ( 1481 )  

    Objective To investigate the efficacy and safety of hemicellulose dressing Veloderm® for wound healing of donor sites of burn or reconstruction. Methods A randomized, open and controlled clinical trial was conducted. Hemicellulose dressing Veloderm® (test group, n=24) and commonly used Vaseline gauze (positive parallel control)(control group, n=24) were applied to the donor sites of burn or reconstruction, respectively. The time of wound healing, and wound healing rates, cumulative healing rates and total effective rates 7 d after operation were compared between two groups, the adverse effects were observed, and the efficacy and safety of Veloderm® for wound healing were evaluated. Results There was significant difference in the healing time of donor sites between test group and control group [(6.25±1.11) d vs (7.00±0.93)d, P<0.05]. The wound healing rate, cumulative healing rate and total effective rate 7 d after operation in test group were significantly higher than those in control group [(97.92±5.69)% vs (93.37±8.92)%, 87.50% vs 58.33%,100% vs 83.33%; P<0.05 for all]. There was no medicine-related adverse effect in both groups. Conclusion Hemicellulose dressing Veloderm® is effective and safe for wound healing of donor sites of burn or reconstruction.

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    Application of low-dose dual-source CT in diagnosis of coronary lesions in patients with Kawasaki disease
    JIN Biao
    2010, 30 (12):  1540. 
    doi: 10.3969/j.issn.1674-8115.2010.12.022

    Abstract ( 2065 )   PDF (6666KB) ( 1571 )  

    Objective To investigate the value of low-dose dual-source CT in the diagnosis of coronary lesions in patients with Kawasaki disease. Methods Thirty-eight children (29 boys and 9 girls) diagnosed as Kawasaki disease underwent coronary CT angiography using dual-source CT. ECG-gated coronary acquisition were applied in 8 patients who could hold breath, and non-ECG-gated conventional thoracic spiral CT were used in 30 patients who could not hold breath. A setting of 80 kV in voltage was implemented. The number, location and size of coronary aneurysms were recorded, and were compared with echocardiographic findings. The radiation doses of patients were calculated. Results A total of 47 coronary aneurysms were demonstrated, with mean diameter of (8.4±3.5) mm and mean length of (13.8±7.4) mm. Ten aneurysms were small, 15 were medium and the other 22 were giant in size. Among the aneurysms in left coronary artery, 8, 13, 5 and 6 were located in the main branch, proximal segment, middle segment and circumflex branch, respectively. Among the aneurysms in right coronary artery, 8, 6 and 1 were located in the proximal, middle and distal segment, respectively. Ten stenotic segments were demonstrated, with calcification in 8 aneurysms. The mean radiation dose was (0.46±0.24)mSv. Echocardiography failed to detect 5 small aneurysms located in the middle and distal segment of coronary arteries. Conclusion Dual-source CT is an useful tool in the diagnosis of coronary lesions in patients with Kawasaki disease, and is a supplement to echocardiography.

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    Original article (Public health administration)
    Investigation on job burnout of medical school teachers and its influential factors
    WANG Fang
    2010, 30 (12):  1545. 
    doi: 10.3969/j.issn.1674-8115.2010.12.023

    Abstract ( 1951 )   PDF (4893KB) ( 1407 )  

    Objective To investigate the job burnout of medical school teachers and its influential factors. Methods One hundred and sixty-four teachers from Shanghai Jiaotong University School of Medicine were investigated with Teacher Demographic Scale, Face Scale, College Teacher Stress Scale, Chinese Maslach Burnout Inventory and Eysenck Personality Questionnaire-R short scale. Results Manifestations of job burnout existed in 66.46% of teachers. The influential factors of emotional exhaustion were neuroticism, working pleasure and working load (P<0.01). The influential factors of depersonalization were job security, psychoticism and extraversion (P<0.05). The influential factors of reduced personal accomplishment were lie and teaching security (P<0.05). Teachers who were younger, bore more social roles, had junior professional rank and less educational experience were more likely to have burnout (P<0.05). Conclusion Job burnout is commonly occurred in medical school teachers. The effect of demographic characteristics on burnout of teachers is not significant, while work stress and personality are primary influential factors.

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    Review
    Relationship between microRNA-155 and malignant tumors
    SHI Lin-jun, ZHOU Zeng-tong, JIANG Wei-wen
    2010, 30 (12):  1550. 
    doi: 10.3969/j.issn.1674-8115.2010.12.024

    Abstract ( 2475 )   PDF (4426KB) ( 1739 )  

    MicroRNAs (miRNAs) are short non-coding RNAs that posttranscriptionally modulate the expression of multiple target genes and are thus implicated in a wide array of cellular and developmental processes. Recently miRNAs and their close associations with malignant tumors remain a major concern. Nowadays microRNA-155(miRNA-155) has been found to be involved in different types of malignant tumors. Here we summarize the research progress of relationship between miRNA-155 and its association with malignant tumors.

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    Clinical value of echocardiography in diagnosis of pulmonary embolism
    ZHANG Xin-yun, JI Xiao-wei, YAN Wei-li, et al
    2010, 30 (12):  1554. 
    doi: 10.3969/j.issn.1674-8115.2010.12.025

    Abstract ( 1520 )   PDF (3174KB) ( 1378 )  

    Pulmonary embolism is an emergency condition with high morbidity and mortality, and many methods can help to diagnosis pulmonary embolism. Echocardiography is of great value in the determination of clinical types of PE and evaluation of hemodynamics of PE and therapeutic effect. Transthoracic echocardiography and transesophageal echocardiography are commonly used clinically, and both possess relatively higher sensitivity and specificity. Echocardiography outperforms the other imaging examinations in the diagnosis of acute pulmonary embolism. The roles of echocardiography in the diagnosis of pulmonary embolism are reviewed in this article.

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    Research progress of DNA methylation and myelodysplastic syndrome
    ZHAO You-shan, CHANG Chun-kang
    2010, 30 (12):  1557. 
    doi: 10.3969/j.issn.1674-8115.2010.12.026

    Abstract ( 1688 )   PDF (8378KB) ( 1142 )  

    Epigenetics is defined as the investigation of heritable changes in gene expression that occurs without a change in DNA seguence. DNA methylation is one of the most important and distinct forms of epigenetic modification. Under usual conditions, there is an inverse correlation between gene expression level and DNA methylation. The gene can be silenced by DNA promoter methylation. Abnormalities of DNA methylation in patients with MDS are closely related to the occurrence, development, prognosis and treatment response of the disease. The mechanism and characteristics of DNA methylation, the abnormalities of DNA methylation in patients with MDS, the effect of DNA methylation in progress of MDS and mechanism and clinical application of demethylating agent are reviewed in this paper.

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    Research progress of osteogenic effects of osteogenic growth peptide
    CHEN Xiao, LUO Bei-er, SU Jia-can
    2010, 30 (12):  1565. 
    doi: 10.3969/j.issn.1674-8115.2010.12.027

    Abstract ( 2083 )   PDF (3927KB) ( 1383 )  

    Osteogenic growth peptide (OGP), which bears osteogenic effects, possesses a great potential in clinical use, and has been the focus of intense investigation worldwide. Both animal experiments in vivo and cellular experiments in vitro have demonstrated that OGP can increase bone density, accelerate fracture healing, promote osteogenic functions of osteoblasts, up-regulate expression of core binding factor alpha 1 and type I collagen mRNA, and stimulate proliferation and differentiation of bone marrow mesenchymal stem cells, finally leading to osteogenesis. The research progress of osteogenetic effects of OGP is reviewed in this paper.

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    Research progress of role of statins in treatment of chronic obstractive pulmonary disease
    ZHOU Jian, LING Mei-rong, DENG Xing-qi
    2010, 30 (12):  1569. 
    doi: 10.3969/j.issn.1674-8115.2010.12.028

    Abstract ( 1656 )   PDF (4533KB) ( 1435 )  

    Chronic obstructive pulmonary disease (COPD) is characterized by progressive and minimally reversible airflow limitation with systemic inflammation, which can be prevented and treated. Statins reduce cholesterol levels by inhibiting 3-hydroxy-3-methlglutaryl coenzyme A(HMG-CoA) reductase, and have an established role in the treatment of cardiovascular diseases. Recent studies have revealed that statins have profound anti-inflammatory effects by reducing induction of matrix metalloproteinase, expression of cytokines and cell adhesion molecules, antioxidant state and C-reactive protein, which decreases lung function deterioration, morbidity and mortality in COPD.

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    Brief original article
    Application of high-frequency ultrasound and three-dimensional color power angiography in diagnosis of hand joint damage of early rheumatoid arthritis
    LI Li, LI Xu-ming, WANG Lei, et al
    2010, 30 (12):  1573. 
    doi: 10.3969/j.issn.1674-8115.2010.12.029

    Abstract ( 1591 )   PDF (4941KB) ( 1543 )  

    Objective To investigate the application of high-frequency ultrasound (HFUS) and three-dimensional color power angiography(3D-CPA)in the diagnosis of hand joint damage in early rheumatoid arthritis (RA). Methods HFUS and 3D-CPA Six were performed for 600 metacarpophalangeal and proximal interphalangeal joints from 30 patients clinically diagnosed as RA. The thickness of synovial membrane and conditions of arthroedema were measured, the damage of tendon and destruction of bone were detected, and the characteristics of blood flow were observed. Besides, the imaging findings of 20 healthy volunteers were served as controls. Results HFUS revealed that there were 458 joints (76.33%) with arthroedema, 429 joints (71.50%) with thickening synovial membrane, 275 joints (45.83%) with peritendinitis of tendon and 134 joints (22.33%) with bone erosion. Vascular hypergenesis (synovium pannus) was found in 86 joints (14.33%) by 3D-CPA. Conclusion HFUS can reveal various appearance of hand joint damage in RA, which can provide a reference for diagnosis. 3D-CPA may have a volumetric display of synovium pannus formation, which is helpful in the diagnosis and treatment of RA.

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