Abstract:

Objective To investigate the effects of Salvia Miltiorrhiza injection on expression of E-cadherin/α-smooth muscle actin (α-SMA) and peroxidative injury of rat peritoneal mesothelial cells induced by high glucose. Methods Rat primary peritoneal mesothelial cells were cultured in vitro. After synchronization for 24 h, primary peritoneal mesothelial cells of the third passage were divided into normal control group, high glucose group (treated with 25 mg/mL glucose), mannitol control group (treated with 25 mg/mL mannitol) and Salvia Miltiorrhiza group (treated with 15 mg/mL Salvia Miltiorrhiza+25 mg/mL glucose). The contents of malondialdehyde (MDA) and reactive oxygen species (ROS) and activity of superoxide dismutase (SOD) in cells were determined, and the expression of E-cadherin and α-SMA mRNA and protein in cells was detected by RT-PCR and Western blotting respectively. Results Compared with normal control group, the contents of ROS and MDA in cells significantly increased, the activity of SOD in cells significantly decreased, the expression of E-cadherin mRNA and protein in cells significantly decreased, and the expression of α-SMA mRNA and protein in cells significantly increased in high glucose group (P<0.05). Compared with high glucose group, the contents of ROS and MDA in cells significantly decreased, the activity of SOD in cells significantly increased, the expression of E-cadherin mRNA and protein in cells significantly increased, and the expression of α-SMA mRNA and protein in cells significantly decreased in Salvia Miltiorrhiza group (P<0.05). Conclusion Salvia Miltiorrhiza injection can significantly reduce the peroxidative injury of peritoneal mesothelial cells induced by high glucose, and its regulation on expression of E-cadherin and α-SMA in cells may be related to the decrease of peritoneal fibrosis.

Key words: Salvia Miltiorrhiza, peritoneal mesothelial cell, peroxidative injury, E-cadherin, α-smooth muscle actin